La casa vital

RESUMEN. Se trata de una investigación sobre algunas relaciones entre la idea y el ejercicio de la arquitectura y el concepto y la praxis de la herida, tanto física como espiritual: sobre la arquitectura como objeto, destino y origen del trauma. Propongo una definición de estas arquitecturas mediante el estudio de obras que se han visto en algún momento de su vida (concebida como la de un humano) - sometidas a procesos de heridas de varios tipos e intensidades, de forma que estos momentos destructivos las han hecho conocer la agonía y, en ocasiones, las han conducido a la extinción. THIS IS AN INVESTIGATION OF SOME RELATIONSHIPS BETWEEN THE IDEA AND THE PRACTICE OF ARCHITECTURE AND THE CONCEPT AND PRACTICE OF INJURY, PHYSICAL AND SPIRITUAL: ABOUT THE ARCHITECTURE LIKE AN OBJECT, DESTINY AND ORIGIN OF THE TRAUMA. I PROPOSE A DEFINITION OF THESE ARCHITECTURES BY STUDYING WORKS THAT HAVE BEEN AT SOME POINT IN THEIR LIFE (CONCEIVED AS A HUMAN) - UNDER TO PROCESSES OF WOUNDS OF VARIOUS TYPES AND INTENSITIES, SO THAT THESE DESTRUCTIVE TIMES HAVE MADE THEM KNOW THE AGONY AND, ON OCCASION, BEEN DRIVEN TO EXTINCTION.

Evaluation of the bioaccumulation kinetics of gold nanorods in vital mammalian organs by mean of TXRF spectrometry

This work presents the first application of total-reflection X-ray fluorescence (TXRF) spectrometry, a new and powerful alternative analytical method, to evaluation of the bioaccumulation kinetics of gold nanorods (GNRs) in various tissues upon intravenous administration in mice. The analytical parameters for developed methodology by TXRF were evaluated by means of the parallel analysis of bovine liver certified reference material samples (BCR-185R) doped with 10 μg/g gold. The average values (n = 5) achieved for gold measurements in lyophilized tissue weight were as follows: recovery 99.7%, expanded uncertainty (k = 2) 7%, repeatability 1.7%, detection limit 112 ng/g, and quantification limit 370 ng/g. The GNR bioaccumulation kinetics was analyzed in several vital mammalian organs such as liver, spleen, brain, and lung at different times. Additionally, urine samples were analyzed to study the kinetics of elimination of the GNRs by this excretion route. The main achievement was clearly differentiating two kinds of behaviors. GNRs were quickly bioaccumulated by highly vascular filtration organs such as liver and spleen, while GNRs do not show a bioaccumulation rates in brain and lung for the period of time investigated. In parallel, urine also shows a lack of GNR accumulation. TXRF has proven to be a powerful, versatile, and precise analytical technique for the evaluation of GNRs content in biological systems and, in a more general way, for any kind of metallic nanoparticles.