Heterologous Expression of a Plant Small Heat-Shock Protein Enhances Escherichia Coli Viability under Heat And Cold Stress Ref.: 1

A small heat-shock protein (sHSP) that shows molecular chaperone activity in vitro was recently purified from mature chestnut (Castanea sativa) cotyledons. This protein, renamed here as CsHSP17.5, belongs to cytosolic class I, as revealed by cDNA sequencing and immunoelectron microscopy. Recombinant CsHSP17.5 was overexpressed in Escherichia coli to study its possible function under stress conditions. Upon transfer from 37°C to 50°C, a temperature known to cause cell autolysis, those cells that accumulated CsHSP17.5 showed improved viability compared with control cultures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of cell lysates suggested that such a protective effect in vivo is due to the ability of recombinant sHSP to maintain soluble cytosolic proteins in their native conformation, with little substrate specificity. To test the recent hypothesis that sHSPs may be involved in protection against cold stress, we also studied the viability of recombinant cells at 4°C. Unlike the major heat-induced chaperone, GroEL/ES, the chestnut sHSP significantly enhanced cell survivability at this temperature. CsHSP17.5 thus represents an example of a HSP capable of protecting cells against both thermal extremes. Consistent with these findings, high-level induction of homologous transcripts was observed in vegetative tissues of chestnut plantlets exposed to either type of thermal stress but not salt stress

Distributed www programming using (Ciao-) prolog and the pillow library

We discuss from a practical point of view a number of ssues involved in writing distributed Internet and WWW applications using LP/CLP systems. We describe PiLLoW, a publicdomain Internet and WWW programming library for LP/CLP systems that we have designed in order to simplify the process of writing such applications. PiLLoW provides facilities for accessing documents and code on the WWW; parsing, manipulating and generating HTML and XML structured documents and data; producing HTML forms; writing form handlers and CGI-scripts; and processing HTML/XML templates. An important contribution of PÍ'LLOW is to model HTML/XML code (and, thus, the content of WWW pages) as terms. The PÍ'LLOW library has been developed in the context of the Ciao Prolog system, but it has been adapted to a number of popular LP/CLP systems, supporting most of its functionality. We also describe the use of concurrency and a highlevel model of client-server interaction, Ciao Prolog's active modules, in the context of WWW programming. We propose a solution for client-side downloading and execution of Prolog code, using generic browsers. Finally, we also provide an overview of related work on the topic.

Pedro Muguruza Otaño (1893-1952): Aproximación histórica a su obra arquitectónica

El arquitecto Pedro Muguruza (1893-1952) fue protagonista de la cultura arquitectónica española de la primera mitad del siglo XX. El estudio de su obra ha sido una carencia dentro de la historiografía de la materia, por lo que la tesis constituye una primera investigación en conjunto de su trayectoria profesional. Muguruza realizó una obra abundante y diversa, pero además fue catedrático de la Escuela de Arquitectura de Madrid, académico de San Fernando y primer director general de arquitectura; datos que corroboran la necesidad de haber desarrollado una monografía con un enfoque global. La tesis ha ordenado, clasificado y analizado sus trabajos, tanto desde un punto de vista histórico como desde el punto de vista arquitectónico. En la investigación se ha recurrido a fuentes primarias y secundarias; y una de las aportaciones de la tesis es el riguroso soporte documental sobre el que se asienta. El trabajo tiene un desarrollo cronológico, dividido en tres capítulos identificados como las etapas generales de su carrera: 1. Años de formación y trayectorias iniciales (1909- 1923), 2. Desarrollo profesional (1923-1938), 3. Las tareas en la administración estatal y último periodo (1938-1952). Asimismo, se ha llevado a cabo el catálogo de su obra —también ordenado cronológicamente—, herramienta que posibilita recorrer de principio a fin el listado y las referencias de su arquitectura. En este apartado se han reproducido más de 1.200 imágenes de 294 proyectos. La tesis ha revisado y descubierto la obra de un arquitecto de mayor recorrido y versatilidad del conocido hasta ahora; personaje complejo de abordar, asociado al franquismo, —cuando en realidad desarrolló una gran parte de sus proyectos en la década de los años veinte y treinta—, difícil de abarcar en todas sus dimensiones, arquitectónica, artística e institucional. La investigación ha permitido indagar y exponer sus proyectos iniciales hasta los de la etapa de madurez, gracias a lo que se han determinado los géneros y los procesos de su trabajo, las constantes de su trayectoria y las referencias e influencias que recibió, tanto de corrientes nacionales como de extranjeras. También se ha revisado su pensamiento arquitectónico a través de sus escritos. El trabajo ofrece un panorama más completo del que entonces se tenía del polifacético Muguruza; arquitecto que se puede presentar como un prototipo reflejo de nuestro pasado reciente, y que al conocer su obra se conoce una parte significativa y destacada de la historia de la arquitectura española contemporánea. ABSTRACT Architect Pedro Muguruza (1893-1952) was a pivotal figure in the Spanish architectural culture of the first half of the 20th century. However, his work as a whole has been insufficiently approached by architectural historiography. This thesis is indeed the first comprehensive research of his professional career. Muguruza’s work is abundant and diverse. He was also a professor at the School of Architecture in Madrid, a luminary scholar at the Royal Academy of Fine Arts of San Fernando and the first General Director of Architecture in Spain. All these facts compel a profound monographic exploration of his far-reaching working path. Muguruza’s projects have been organized, classified and analyzed in this thesis both from historical and architectural points of view. Primary and secondary sources have been scrutinized throughout this research and one of the assets of this project is the rigorous documentation on which it is firmly grounded. The results of this research have been chronologically organized and structured into three chapters identified with the general stages of his prolific career: 1. Training years and his initial paths (1909-1923). 2. Professional development (1923-1938). 3. His role in the state administration and his last period (1938-1952). A chronological catalogue of all his architectural work has also been carried out as part of this project. This catalogue offers the possibility to track his works and all references to them under a historical perspective. More than 1,200 images of his projects have been included in this catalogue. The thesis has gone through and shown up the work of an architect whose magnitude and versatility is much more than what previously known. Muguruza represents a complicated figure to approach ideologically speaking because of his later relationship to Franco’s regime – when in fact Muguruza developed a big part of his projects in the 20’s and 30’s before the dictatorship –. He is also a difficult figure to research on because of his exhaustive working activity in different dimensions: architectural, artistic and institutional. This research allows any incoming scholars to approach Muguruza’s projects under an accurate perspective. From his initial works to his maturity period, his projects have been analyzed and classified by establishing different stages. Additionally, some constant guidelines within his long career and the influences he received both in Spain and abroad have also been identified and properly considered. His architectural line of thought has been explored through his writings too. This thesis provides a complete overview of a multifaceted Muguruza, an architect who can be introduced as an archetype of our recent cultural heritage. By exploring his work a quintessential part of the Spanish contemporary architecture is definitively revealed and acknowledged.