Effect of row orientation on soil water content and vine water status on a Cabernet franc vineyard in Madrid, spain

The aim of this study was to evaluate the effects of row orien¬tation on vine and soil water status in an irrigated vineyard. The trial was developed during 2006, 2007 and 2008, in the South East region of Madrid (Spain) on 5-year old Cabernet franc grapevines (Vitis vinifera L.) grafted onto 140Ru. Plant spacing was 2.5 m x 1.5 m and vines were trained to a VSP. Four orientations were stu¬died: North-South (N-S), East-West (E-W), Northeast-Southwest (N+45) and North-South +20o (N+20). Irrigation (0.4•ET0) started when shoot growth stopped. Soil water availability was measured using a TDR technique with forty buried probes. Row orientation did not have any effect on water consumption in the vineyard. At maturity, leaf water potential was measured at predawn, early mor¬ning, midday and 14:00 solar time, on both canopy sides - sun and shade – ; the early morning measurement was the one that better differentiated treatments. Leaf water potential was a good indica¬tor of plant water status. Differences between (N-S and E-W) and (N+20 and N+45) treatments were obtained both on sun and shade canopy sides, N+20 and N+45 having lower leaf water potentials then drier leaves. The water stress integral shows that N-S and E-W reach the end of maturation with a greater level of hydration than N+45 and N+20. As a whole, N+45 and N+20 orientations, without affecting too much the soil available water content, induce regularly more water stress to the vine at some periods, probably due to an higher sunlight interception in early morning which makes water limitation for the vine more early and thus more severe during the day.

Characterization of vineyard's canopy through fuzzy clustering and svm over color images

In this work we propose an image acquisition and processing methodology (framework) developed for performance in-field grapes and leaves detection and quantification, based on a six step methodology: 1) image segmentation through Fuzzy C-Means with Gustafson Kessel (FCM-GK) clustering; 2) obtaining of FCM-GK outputs (centroids) for acting as seeding for K-Means clustering; 3) Identification of the clusters generated by K-Means using a Support Vector Machine (SVM) classifier. 4) Performance of morphological operations over the grapes and leaves clusters in order to fill holes and to eliminate small pixels clusters; 5)Creation of a mosaic image by Scale-Invariant Feature Transform (SIFT) in order to avoid overlapping between images; 6) Calculation of the areas of leaves and grapes and finding of the centroids in the grape bunches. Image data are collected using a colour camera fixed to a mobile platform. This platform was developed to give a stabilized surface to guarantee that the images were acquired parallel to de vineyard rows. In this way, the platform avoids the distortion of the images that lead to poor estimation of the areas. Our preliminary results are promissory, although they still have shown that it is necessary to implement a camera stabilization system to avoid undesired camera movements, and also a parallel processing procedure in order to speed up the mosaicking process.

Importance of canopy porosity into vineyard and the relationship with the grape maturity. Digital estimation method

In warm and dry climates, the use of porous systems should be required in order to allow a better leaf distribution inside the plant, causing more space in the clusters area and enhancing determined physiological processes so in the leaf (photosynthesis, v entilation, transpiration) as in berry (growth and maturation). Plant geometry indexes, yield and must composition have been studied in three different systems: sprawl with 12 shoots/m (S1); sprawl system with 18 shoots/m (S2) and vertical positioned syste m or VSP with 12 shoots/m (VSP1). Total leaf area increases as the crop load does, whoever surface area depends on to two factors: crop load and the training system (VSP vs. sprawl), which can provide differences in leaf exposure efficiencies. The main objective of this study was to validate digital photography measurements used to compare porosity differences among treatments and, as they affect plant microclimate and, therefore, yield and berry quality. Also, all previous studied indexes (LAI, SA, SFEr) tended to overestimate the relationship between exposed leaf surface and porosity of each treatment, but the use of digital method proved to be an effective tool in order to assess canopy porosity. Results showed that not positioned and free systems (sprawl) scored between 25- 50% more porosity in the clusters area than the fixed vertical system (VSP), which resulted in a better plant microclimate for test conditions, mainly by improving the exposure of internal clusters and internal canopy ventilation. On the other hand, higher crop load treatment (S2) showed a real increase in yield (16%) without any relevant change into must composition, even improving total anthocyanin content into berry during ripening

Importance of canopy porosity into vineyard and the relationship with the grape maturity. Digital estimation method

In warm and dry climates, the use of porous systems should be required in order to allow a better leaf distribution inside the plant, causing more space in the clusters area and enhancing determined physiological processes so in the leaf (photosynthesis, ventilation, transpiration) as in berry (growth and maturation). Plant geometry indexes, yield and must composition have been studied in three different systems: sprawl with 12 shoots/m (S1); sprawl system with 18 shoots/m (S2) and vertical positioned system or VSP with 12 shoots/m (VSP1). Total leaf area increases as the crop load does, whoever surface area depends on to two factors: crop load and the training system (VSP vs . sprawl), which can provide differences in leaf exposure efficiencies. The main objective of this study was to validate digital photography measurements used to compare porosity differences among treatments and, as they affect plant microclimate and, therefore, yield and berry quality. Also, all previous studied indexes (LAI, SA, SFEr) tended to overestimate the relationship between exposed leaf surface and porosity of each treatment, but the use of digital method proved to be an effective tool in order to assess canopy porosity. Results showed that not positioned and free systems (sprawl) scored between 25 - 50% more porosity in the clusters area than the fixed vertical system (VSP), which resulted in a better plant microclimate for test conditions, mainly by improving the exposure of internal clusters and internal canopy ventilation. On the other hand, higher crop load treatment (S2) showed a real increase in yield (16%) without any relevant change into must composition, even improving total anthocyanin content into berry during ripening

Morphological Functions to Quantify Three-Dimensional Tomograms of Macropore Structure in a Vineyard Soil with Two Different Management Regimes

Soil tomography and morphological functions built over Minkowski functionals were used to describe the impact on pore structure of two soil management practices in a Mediterranean vineyard. Soil structure controls important physical and biological processes in soil–plant–microbial systems. Those processes are dominated by the geometry of soil pore structure, and a correct model of this geometry is critical for understanding them. Soil tomography has been shown to provide rich three-dimensional digital information on soil pore geometry. Recently, mathematical morphological techniques have been proposed as powerful tools to analyze and quantify the geometrical features of porous media. Minkowski functionals and morphological functions built over Minkowski functionals provide computationally efficient means to measure four fundamental geometrical features of three-dimensional geometrical objects, that is, volume, boundary surface, mean boundary surface curvature, and connectivity. We used the threshold and the dilation and erosion of three-dimensional images to generate morphological functions and explore the evolution of Minkowski functionals as the threshold and as the degree of dilation and erosion changes. We analyzed the three-dimensional geometry of soil pore space with X-ray computed tomography (CT) of intact soil columns from a Spanish Mediterranean vineyard by using two different management practices (conventional tillage versus permanent cover crop of resident vegetation). Our results suggested that morphological functions built over Minkowski functionals provide promising tools to characterize soil macropore structure and that the evolution of morphological features with dilation and erosion is more informative as an indicator of structure than moving threshold for both soil managements studied.

Parallel sets and morphological measurements of CT images of soil pore structure in a vineyard

Important physical and biological processes in soil-plant-microbial systems are dominated by the geometry of soil pore space, and a correct model of this geometry is critical for understanding them. We analyze the geometry of soil pore space with the X-ray computed tomography (CT) of intact soil columns. We present here some preliminary results of our investigation on Minkowski functionals of parallel sets to characterize soil structure. We also show how the evolution of Minkowski morphological measurements of parallel sets may help to characterize the influence of conventional tillage and permanent cover crop of resident vegetation on soil structure in a Spanish Mediterranean vineyard.

Effects of soil management in vineyard on soil physical and chemical characteristics

Cover crops in Mediterranean vineyards are scarcely used due to water competition between the cover crop and the grapevine; however, bare soil management through tillage or herbicides tends to have negative effects on the soil over time (organic matter decrease, soil structure and soil fertility degradation, compaction, etc). The objective of this study was to understand how soil management affects soil fertility, compaction and infiltration over time. To this end, two bare soil techniques were compared, tillage (TT) and total herbicide (HT) with two cover crops; annual cereal (CT) and annual grass (AGT), established for 8 years. CT treatment showed the highest organic matter content, having the biggest amount of biomass incorporated into the soil. The annual adventitious vegetation in TT treatment (568 kg dry matter ha-1) that was incorporated into the soil, kept the organic matter content higher than HT levels and close to AGT level, in spite of the greater aboveground annual biomass production of this treatment (3632 kg dry matter ha-1) whereas only its roots were incorporated into the soil. TT presented the highest bulk density under the tractor track lines and a greatest resistance to penetration (at 0.2 m depth). AGT presented bulk density values (upper 0.4 m) lower than TT and penetration resistance in CT lower (at 0.20 m depth) than TT too. Effects of soil management in vineyard on soil physical and chemical characteristics - ResearchGate. Available from: http://www.researchgate.net/publication/268520480_Effects_of_soil_management_in_vineyard_on_soil_physical_and_chemical_characteristics [accessed May 20, 2015].

Agronomic performance of Graciano, Petit verdot and Tempranillo grapevine cultivars (Vitis vinífera L.) under two water regimes.

Fort the last decades Spanish vineyard has experience a deep transformation by allowing irrigation, changing the traditional bush into a possible full mechanized VSP and cultivar changing map was also took into account. Tempranillo has led the change to replace the white grapevines, such as in La Mancha; however results in these warm areas were not as expect. Under this conditions, low yield, low acidity and uncouple sacharimetric and phenol maturity usually is obtained. Within this frame an experimental trial was set up with the goal to know other cultivars that could better perform than Tempranillo in warm areas in a sustainable viticulture.

Estudio del empleo de cubiertas vegetales temporales para la regulación del régimen hídrico, crecimiento y manejo sostenible del viñedo

RESUMEN El ensayo se llevo a acabo en un viñedo de Syrah durante 8 años y en un viñedo de Merlot durante 3 años. Ambos viñedos regados y situados en Colmenar de Oreja (Madrid) (40º 8’N, 3º 23’W) con clima típicamente Mediterráneo. Siete tratamientos con cubiertas vegetales se han comparado con dos tratamientos con suelo desnudo usados como control. Las cubiertas vegetales fueron seis tratamientos de cereales (Centeno) y un tratamiento de enyerbado autosembrado (Bromus spp) y los tratamientos de suelo desnudo fueron uno manejado con laboreo y otro manejado con herbicida. Los seis tratamientos de centeno se han manejado de seis formas distintas. La primera sembrada todos los años y eliminada en brotación mediante herbicida de post-emergencia. La segunda sembrada todos los años y eliminada un mes después de la brotación mediante siega. La tercera sembrada todos los años y eliminada en floración mediante siega. La cuarta sembrada todos los años y eliminada en brotación mediante herbicida de post-emergencia. La quinta sembrada todos los años y eliminada un mes después de la brotación mediante siega. La sexta sembrada todos los años y eliminada en floración mediante siega. La utilización de cubiertas vegetales ha tenido efectos beneficiosos sobre el contenido en materia orgánica, la compactación y la infiltración del suelo, mejorando las condiciones para el desarrollo de las raíces. Estas mejoras y la escasa competencia de la competencia durante el crecimiento del sistema radical de la vid han producido un incremento del sistema radical en las plantas mantenidos con cubierta vegetal. La competencia de las cubiertas vegetales ha reducido la disponibilidad hídrica de la vid, incrementándose la absorción en zonas con mayor disponibilidad hídrica (como la línea) antes de floración. El mayor desarrollo radical de las vides con cubierta autosembrada ha permitido agotar más intensamente las reservas de agua en el suelo. La competencia de las cubiertas ha reducido en mayor medida el desarrollo vegetativo que el productivo. Lo que ha disminuido, en algunas cubiertas vegetales, el consumo hídrico de la vid, aumentando el potencial hídrico foliar y la fotosíntesis durante la maduración. Sin embargo, el incremento en la fotosíntesis no ha compensado el mayor desarrollo foliar de los tratamientos con suelo desnudo, lo que ha provocado que estos tratamientos presenten la producción de materia seca más elevada. El empleo de cubiertas vegetales ha reducido la producción principalmente limitando el número de bayas por racimo, ya que el aporte de riego ha minimizado los efectos del manejo del suelo sobre el tamaño de baya. La utilización de cubiertas vegetales temporales ha mejorado la iluminación de los racimos, lo que ha producido un aumento de la síntesis de antocianos durante las primeras fases de la maduración, pero un incremento de la degradación de los mismos al final de la maduración. Esto ha provocado que durante la vendimia los tratamientos de suelo desnudo presenten un mayor contenido de antocianos por baya que los tratamientos mantenidos con cubierta temporal. Estos resultados muestran que el efecto del manejo del suelo depende en gran medida de las condiciones del medio, y que sus efectos en climas calidos y secos son muy distintos a los observados en climas frescos y húmedos. ABSTRACT The trial was conducted over a period of 8 years in a Syrah vineyard and over a period of 3 years in a Merlot vineyard. Both vineyards were irrigated and situated near Colmenar de Oreja (Madrid) (40º 8’N, 3º 23’W) a typical Mediterranean climate. Seven Annual cover crops treatments were compared to two bare soil treatments, used as control. Cover crops were six cereals treatments (Rye) and one auto-sowing treatment (Bromus spp) and the treatments of bare soil were one tilled management treatment and another with herbicide treatment. The six Cereal treatments were managed in different manners. First sowing every year and were eliminated in bud breaking with post-emergency herbicide. The second sowing annually and were eliminated one month after bud breaking through harvesting. The third sowing annually and were eliminated in flowering by mowing. The fourth sowing annually and were eliminated with post-emergency herbicide in bud breaking. The fifth sowing annually and were eliminated by mowing one month after bud breaking. . The third sowing annually was eliminated by mowing in flowering. The use of annual cover crop have improved soil organic matter, soil infiltration rate and soil solidity, resulting in a more favourable environment for roots growth. These improvements and low competitive ability during root growing have increases grapevine root density in plant management with cover crop. The Cover crop ability reduced plant available water, increasing root water uptake in the soil with more available water (such us line) before flowering. More growth of grapevine root density with auto-sowed cover crops has allowed using the water under soil more rapidly. The cover crop ability has reduced vegetative growth more than yield. What has been reduced in some vegetative cover crop has been the consumption of water, and increasing the leaf water potential and foliar and photosynthesis during growth activity. Moreover, the increased in photosynthesis activity could not “Compensate” higher leaf growth of treatment of bare soil, where these treatments had resulted in the greatest amount of dry material. The use of cover crops has reduced the crop mainly reducing the fruit set, because the irrigation had reduced the cover crop effect in the berry growth. The use of temporary cover crop increased berry sunlight exposure and skin anthocyanin synthesis during early rippenig, but excessively high temperature increased anthocyanin degradation during last part of ripenning. So, at the vineyard harvest period the treatments with bare soil plant had a more anthocyanin content per grape than the temporary cover crop plant treatments. These results suggest that the effects of soil handling mainly depends on the environmental condition, and their effects in hot and dry climate are so different from the effects in cold and moist climates.

Evapotranspiration of grapevine trained to a gable trellis system under netting and black plastic mulching

The evapotranspiration (ET c) of a table grape vineyard (Vitis vinifera, cv. Red Globe) trained to a gable trellis under netting and black plastic mulching was determined under semiarid conditions in the central Ebro River Valley during 2007 and 2008. The netting was made of high-density polyethylene (pores of 12 mm2) and was placed just above the ground canopy about 2.2 m above soil surface. Black plastic mulching was used to minimize soil evaporation. The surface renewal method was used to obtain values of sensible heat flux (H) from high-frequency temperature readings. Later, latent heat flux (LE) values were obtained by solving the energy balance equation. For the May–October period, seasonal ET c was about 843 mm in 2007 and 787 mm in 2008. The experimental weekly crop coefficients (K cexp) fluctuated between 0.64 and 1.2. These values represent crop coefficients adjusted to take into account the reduction in ET c caused by the netting and the black plastic mulching. Average K cexp values during mid- and end-season stages were 0.79 and 0.98, respectively. End-season K cexp was higher due to combination of factors related to the precipitation and low ET o conditions that are typical in this region during fall. Estimated crop coefficients using the Allen et al. (1998) approach adjusting for the effects of the netting and black plastic mulching (K cFAO) showed a good agreement with the experimental K cexp values.

Grapevine Yield and Leaf Area Estimation Using Supervised Classification Methodology on RGB Images Taken under Field Conditions

The aim of this research was to implement a methodology through the generation of a supervised classifier based on the Mahalanobis distance to characterize the grapevine canopy and assess leaf area and yield using RGB images. The method automatically processes sets of images, and calculates the areas (number of pixels) corresponding to seven different classes (Grapes, Wood, Background, and four classes of Leaf, of increasing leaf age). Each one is initialized by the user, who selects a set of representative pixels for every class in order to induce the clustering around them. The proposed methodology was evaluated with 70 grapevine (V. vinifera L. cv. Tempranillo) images, acquired in a commercial vineyard located in La Rioja (Spain), after several defoliation and de-fruiting events on 10 vines, with a conventional RGB camera and no artificial illumination. The segmentation results showed a performance of 92% for leaves and 98% for clusters, and allowed to assess the grapevine’s leaf area and yield with R2 values of 0.81 (p < 0.001) and 0.73 (p = 0.002), respectively. This methodology, which operates with a simple image acquisition setup and guarantees the right number and kind of pixel classes, has shown to be suitable and robust enough to provide valuable information for vineyard management.

Sum Insured Determination for Cereal, Citrus and Vineyards in the Spanish Agricultural Insurance System.

In general, insurance is a form of risk management used to hedge against a contingent loss. The conventional definition is the equitable transfer of a risk of loss from one entity to another in exchange for a premium or a guaranteed and quantifiable small loss to prevent a large and possibly devastating loss being agricultural insurance a special line of property insurance. Agriculture insurance, as actually are designed in the Spanish scenario, were established in 1978. At the macroeconomic insurance studies scale, it is necessary to know a basic element for the insurance actuarial components: sum insured. When a new risk assessment has to be evaluated in the insurance framework, it is essential to determinate venture capital in the total Spanish agriculture. In this study, three different crops (cereal, citrus and vineyards) cases are showed to determinate sum insured as they are representative of the cases found in the Spanish agriculture. Crop sum insured is calculated by the product of crop surface, unit surface production and crop price insured. In the cereal case, winter as spring cereal sowing, represents the highest Spanish crop surface, above to 6 millions of hectares (ha). Meanwhile, the four citrus species (oranges, mandarins, lemons and grapefruits) occupied an extension just over 275.000 ha. On the other hand, vineyard target to wine process shows almost one million of ha in Spain.

Análisis de las diferencias en el crecimiento vegetativo, en los componentes del rendimiento y en la composición de las uvas y vinos del cv. cabernet sauvignon (vitis vinifera L.), producidos por el déficit hídrico durante pre-envero y post-envero

La presente Tesis Doctoral se realizó con el fin de estimar conjuntamente la respuesta agronómica y fisiológica de la vid (Vitis vinifera L.), así como los efectos sobre la evolución de la maduración, composición y la calidad de la uva y del vino, bajo la aplicación de diferentes déficit hídricos en pre-envero y post-envero, dentro de un marco de referencia de cambio climático. La variación climática que prevén los estudios sobre el cambio climático, resulta un factor decisivo en la eficiencia del uso del agua en la vid. En zonas cálidas, las estrategias de cultivo del viñedo frente al cambio climático deben de ir dirigidas a atenuar sus efectos sobre el crecimiento y el desarrollo de la vid, haciéndose imprescindible el estudio pormenorizado del déficit hídrico como factor decisivo en la obtención de las uvas adecuadas, ya que son la clave indispensable para el éxito en la elaboración del vino, y de forma muy especial en los vinos enfocados a un sector de alta calidad. El ensayo se llevó a cabo en un viñedo comercial de Bodegas Licinia, en la Comunidad de Madrid, durante los años 2010 y 2011. La variedad estudiada fue Cabernet sauvignon / 41 B, plantada a un marco de plantación de 3 m x 1 m, con un guiado vertical de la vegetación. El dispositivo experimental fue totalmente al azar, y se establecieron 4 tratamientos experimentales con 4 grados de disponibilidad hídrica, déficit moderado continuo (T0,45-0,6), déficit severo continuo (T0-0,3), déficit severo después de envero (T0,45-0,3) y déficit severo antes de envero (T0-0,6). En cada tratamiento se distribuyeron 3 repeticiones. El año 2010 fue el más lluvioso de los años de ensayo, con 478 mm de precipitaciones anuales, lo que supuso 146 mm más que en el año 2011. Su distribución a lo largo del ciclo fue más homogénea en el año 2010, mientras que en 2011 las precipitaciones contabilizadas en el período de maduración de la uva fueron nulas. La temperatura media subió 0,9ºC en 2011, respecto a 2010 y en cuanto a la integral térmica eficaz, en 2011 se acumularon, desde el 1 de abril hasta el final de ciclo, 217 grados•día más que en 2010. El déficit hídrico en pre-envero, modificó notablemente el crecimiento vegetativo y la producción de cosecha de la parcela de ensayo, no así la fertilidad de las yemas. El tratamiento con mayor disponibilidad hídrica (T0,45-0,6) obtuvo el mayor peso de baya, y los tratamientos con menor déficit hídrico en pre-envero (T0,45-0,6 y T0,45-0,3) registraron los mayores rendimientos de cosecha, mientras que las menores tasas de cuajado correspondieron al tratamiento con un déficit severo continuo (T0-0,3). La parcela de ensayo se caracterizó por un exceso de vigor y un alto crecimiento vegetativo. El pH del mosto se vio afectado por el déficit hídrico, disminuyendo su valor en el tratamiento de déficit hídrico severo antes de envero (T0-0,6). Organolépticamente, no se percibieron diferencias significativas en los vinos elaborados en función del déficit hídrico, y respecto a su composición físico-química, solo existieron diferencias en la concentración de ácido L-Málico, con mayores concentraciones en los tratamientos sin déficit hídrico en pre-envero, T0,45-0,6 y T0,45-0,3. El déficit hídrico modificó notablemente el color del vino, aumentando los valores de las coordenadas CIELAB a* y b*, la luminosidad (L*), croma (C*) y tonalidad (H*), para los tratamientos con un déficit severo en pre-envero (T0-0,3 y T0-0,6) y disminuyendo estas en el tratamiento con mayor disponibilidad hídrica (T0,45-0,6). Del mismo modo, mediante el análisis de color por métodos tradicionales, IPT e IC de los vinos, aumentó en los tratamientos con mayor déficit hídrico en pre-envero (T0-0,3 y T0-0,6), respecto a los tratamientos de mayor disponibilidad (T0,45-0,6 y T0,45-0,3). La concentración de taninos de la baya en vendimia, no se vio afectada por el déficit hídrico, aunque sí estuvo relacionada positivamente con el tamaño de las bayas. Organolépticamente, los hollejos del año 2011 resultaron con menor frescura, acidez, afrutado, sensación herbácea e intensidad tánica, aunque con mayor astringencia respecto a 2010. Las pepitas fueron más astringentes y aromáticas pero menos crujientes, sin llegar a los niveles de madurez del año 2010. El catador relacionó los taninos con la calidad del vino, asociándolos con un mayor cuerpo, acidez, intensidad, equilibrio gustativo, amargor y menor astringencia en la fase gustativa. La concentración de taninos en los vinos se vio favorecida con el déficit hídrico en pre-envero y post-envero. Los tratamientos con mayor déficit hídrico en pre-envero, T0-0,6 y T0-0,3, obtuvieron las menores concentraciones de potasio en mostos y vinos. Las relaciones entre la concentración de potasio, ácido L-Málico y el porcentaje de color rojo puro (dA(%)) resultaron altamente significativas, de modo que las mayores tasas de potasio en el vino se asociaron a los valores más bajos de color rojo y a los mayores de ácido L-Málico. ABSTRACT The present Doctoral Thesis has been done in order to estimate the grapevine (Vitis vinifera L.) agronomic and physiologic performance or response as well as the impact in the grape and wine maturity, composition and quality evolution, with different water deficits. The variation in climate that the global warming studies for seen is a key factor for the grapevine water use efficiency. In warm areas the farming vineyards strategy to face the climatic change, should be focused on diminish the effects on the grapevine growth and development, so that the water deficit detailed analysis becomes decisive to obtain the appropriate grapes, that are the main subject for a successful wine production and especially for top quality wines. The trial was carried out in a commercial vineyard in Chinchón (Madrid), Licinia winery, during the 2010 and 2011 seasons. The grape variety studied was Cabernet Sauvignon grafted onto 41B with a vine spacing 3m x 1m trained as VSP. Experimental design consisted on 4 irrigation treatments with 3 replications totally randomized. Irrigation treatments were: moderate regulated deficit (T0,45-0,6), severe continuous deficit (T0-0,3), severe post-veraison deficit (T0,45-0,3) and severe pre veraison deficit (T0-0,6). The 2010 was rainier year than the 2011; Total annual rain in 2010 was 478 mm, which resulted in 146 mm more than in 2011. The distribution along the vine cycle was more homogeneous in the 2010, whereas precipitations in 2011 along the grape maturity period were nonexistent. The average temperature in 2011 was 0,9ºC higher than that of the 2010 and regarding to the thermal integral, in the 2011 from 1st April to the end of the growing cycle, was 217 degrees•day higher than that in 2010. Water deficit significantly modified the vegetative growth and yield but, it did not modified bud fertility. The treatment with the highest water availability (T0,45-0,6) got the highest berry size, the lowest berry set rates were found in the severe continuous deficit treatment (T0-0,3). The plot studied in this trial was characterized by both excessive vigour and vegetative growth. Water deficit modified the pH must by, reducing it in the severe water deficit during pre-veraison (T0-0,6). There were not differences in wine tasting between the water deficits treatments. Regarding to the physical-chemical composition, it only existed differences in the L-malic acid concentration, resulting higher concentrations in the water deficit pre-veraison treatments: T0,45-0,6 y T0,45-0,3. Water deficit significantly modified wine colour by, increasing the CIELAB coordinates a* and b*, the brightness (L*), croma (C*) and tonality (H*), in the lower water availability pre-veraison treatments (T0-0,3 y T0-0,6), and reducing them in the in the moderate continuous water deficit ones (T0,45-0,6). By means of traditional wine colour parameters analyses, red colour percentage, TPI, they became higher in the lower water availability pre-veraison treatments (T0-0,3 y T0-0,6), than in those with higher availability (T0,45-0,6 y T0,45-0,3). At harvest, berry tannins concentrations was not affected by the water deficit although it did in a positive way, in the berry size. Berry tasting in 2011, resulted in a lower freshness, acidity, fruity, herbaceous flavour and tannic intensity, but with higher astringency respect to the 2010 season. Seeds, in 2011, were more astringent and aromatic as in the 2010, but less crunchy, without getting to the point of maturity. The taster linked the tannins to wine quality, associating them with a higher bodiest wine, acidity, intensity, taste balance, bitterness and with a lower astringency in the tasting stage. Treatments with a higher water deficit up to veraison T0-0,6 y T0-0,3 got less musts and wines potassium concentration. The relation between L-malic acid and the full red color percentage (dA(%)), were highly related, resulting the higher potassium content the lower wine quality.

Effects of irrigation and time of application on the yield and quality of cv. Cabernet sauvignon in a warm climate

Mediterranean climate is characterized by hot summer, high evapotranspiration rates, and scarce precipitations (400 mm per year) during grapevine cycle. These extremely dry conditions affect vineyard productivity and sustainability. Supplementary irrigation is a needed practice in order to maintain yield and quality. Almost all Spanish grape growing regions are characterized by these within this context, especially in the center region, where this study was performed. The main objective of this work was to study the influence of irrigation on yield and quality. For this aim, we applied different levels of irrigation (mm of water applied) during different stages of growth and berry maturity. Four experimental treatments were applied considering the amount of water and the moment of the application: T1: Water irrigation (420 mm) applied from bloom to maturity. T2: Corresponded to the traditional irrigation scheduling, from preveraison to maturity (154 mm). T3: Water irrigation from bloom to preveraison, and water deficit from veraison to maturity (312 mm). T4: Irrigation applied from preveraison to maturity (230 mm) Experimental vineyard, cv. Cabernet Sauvignon, was located in a commercial vineyard (Bodegas Licinia S.L.) in the hot region of Morata de Tajuña (Madrid). The trial was performed during 2010 and 2011 seasons. Our results showed that yield increased from 2010 to 2011 in the treatments with a higher amount of water appli ed, T1 and T3 (24 and 10 % of yield increase respectively). This was mainly due to an increase in bud fertility (nº of bunches per shoot). Furthermore, sugar content was higher in T3 (27.3 ºBrix), followed by T2 (27 ºBrix). By contrast, T4 (irrigation from veraison) presented the lowest solid soluble concentration and the highest acidity. These results suggest that grapevine has an intrinsic capacity to adapt to its environment. However, this adaptation capacity should be evaluated considering the sensibility of quality parameters during the maturity period (acidity, pH, aroma, color...) and its impact on yield. Here, we demonstrated that a higher amount of water irrigation applied was no linked to a negative effect on quality.

Comparison of different pre-treatments to improve accuracy of total soluble solids.

The applicability of a portable NIR spectrometer for estimating the °Brix content of grapes by non-destructive measurement has been analysed in field. The NIR spectrometer AOTF-NIR Luminar 5030, from Brimrose, was used. The spectrometer worked with a spectral range from 1100 to 2300 nm. A total of 600 samples of Cabernet Sauvignon grapes, belonging to two vintages, were measured in a non-destructive way. The specific objective of this research is to analyse the influence of the statistical treatment of the spectra information in the development of °Brix estimation models. Different data pretreatments have been tested before applying multivariate analysis techniques to generate estimation models. The calibration using PLS regression applied to spectra data pretreated with the MSC method (multiplicative scatter correction) has been the procedure with better results. Considering the models developed with data corresponding to the first campaign, errors near to 1.35 °Brix for calibration (SEC = 1.36) and, about 1.50 °Brix for validation (SECV = 1.52) were obtained. The coefficients of determination were R2 = 0.78 for the calibration, and R2 = 0.77 for the validation. In addition, the great variability in the data of the °Brix content for the tested plots was analysed. The variation of °Brix on the plots was up to 4 °Brix, for all varieties. This deviation was always superior to the calculated errors in the generated models. Therefore, the generated models can be considered to be valid for its application in field. Models were validated with data corresponding to the second campaign. In this sense, the validation results were worse than those obtained in the first campaign. It is possible to conclude in the need to realize an adjustment of the spectrometer for each season, and to develop specific predictive models for every vineyard.