Climatic factors control rodent seed predation in Pinus pinea L. stands in Central Spain

- Context: Pinus pinea L. presents serious problems of natural regeneration in managed forest of Central Spain. The species exhibits specific traits linked to frugivore activity. Therefore, information on plant–animal interactions may be crucial to understand regeneration failure. - Aims: Determining the spatio-temporal pattern of P. pinea seed predation by Apodemus sylvaticus L. and the factors involved. Exploring the importance of A. sylvaticus L. as a disperser of P. pinea. Identifying other frugivores and their seasonal patterns. - Methods: An intensive 24-month seed predation trial was carried out. The probability of seeds escaping predation was modelled through a zero-inflated binomial mixed model. Experiments on seed dispersal by A. sylvaticus were conducted. Cameras were set up to identify other potential frugivores. - Results: Decreasing rodent population in summer and masting enhances seed survival. Seeds were exploited more rapidly nearby parent trees and shelters. A. sylvaticus dispersal activity was found to be scarce. Corvids marginally preyed upon P. pinea seeds. - Conclusions: Survival of P. pinea seeds is climate-controlled through the timing of the dry period together with masting occurrence. Should germination not take place during the survival period, establishment may be limited. A. sylvaticus mediated dispersal does not modify the seed shadow. Seasonality of corvid activity points to a role of corvids in dispersal.

Leftovers in seed dispersal: ecological implications of partial seed consumption for oak regeneration

1. Successful seed dispersal by animals is assumed to occur when undamaged seeds arrive at a favourable microsite. Most seed removal and dispersal studies consider only two possible seed fates, predation or escape intact. Whether partial consumption of seeds has ecological implications for natural regeneration is unclear. We studied partial consumption of seeds in a rodent-dispersed oak species. 2. Fifteen percent of dispersed acorns were found partially eaten in a field experiment. Most damage affected only the basal portion of the seeds, resulting in no embryo damage. Partially eaten acorns had no differences in dispersal distance compared to intact acorns but were recovered at farther distances than completely consumed acorns. 3. Partially eaten acorns were found under shrub cover unlike intact acorns that were mostly dispersed to open microhabitats. 4. Partially eaten acorns were not found buried proportionally more often than intact acorns, leading to desiccation and exposure to biotic agents (predators, bacteria and fungi). However, partial consumption caused more rapid germination, which enables the acorns to tolerate the negative effects of exposure. 5. Re-caching and shrub cover as microhabitat of destination promote partial seed consumption. Larger acorns escaped predation more often and had higher uneaten cotyledon mass. Satiation at seed level is the most plausible explanation for partial consumption. 6. Partial consumption caused no differences in root biomass when acorns experienced only small cotyledon loss. However, root biomass was lower when acorns experienced heavy loss of tissue but, surprisingly, they produced longer roots, which allow the seeds to gain access sooner to deeper resources. 7.Synthesis. Partial consumption of acorns is an important event in the oak regeneration process, both quantitatively and qualitatively. Most acorns were damaged non-lethally, without decreasing both dispersal distances and the probability of successful establishment. Faster germination and production of longer roots allow partially eaten seeds to tolerate better the exposure disadvantages caused by the removal of the pericarp and the non-buried deposition. Consequently, partially consumed seeds can contribute significantly to natural regeneration and must be considered in future seed dispersal studies.

Disentangling factors controlling fruit and seed removal by rodents in temperate forests

Fleshy fruits fall on to the ground together with cleaned seeds previously ingested by primary dispersers, offering a wide range of fruits and seeds to the ground foragers. Although nutritional properties strongly differ between fruits and seeds, this different seed presentation (cleaned seeds versus seeds within the pulp) has not been addressed in seed removal studies. This study reports on the removal of fruits versus their seeds in five fleshy-fruited species in a temperate forest. We found that rodents removed most of the seeds and partially consumed most of the fruits, preferring seeds to fruits. Rodents bit the fruits to extract the seeds, leaving most of the pulp. We found a preference ranking for the seeds (Sorbus aucuparia>Ilex aquifolium>Sorbus aria>Rosa canina>Crataegus monogyna) but no preferences were found for the fruits, probably due to their similarities in pulp constituents. Seed and fruit choice were affected by chemical and physical properties and not by their size. The presence of alternative and preferred seeds (nuts) delayed the encounter of the fruits and seeds and diminished their removal rates. We found that higher rodent abundance is not necessarily associated with higher removal rates of fleshy fruits. Rodent abundance, fruit size and seed size are minor factors in the removal of fleshy fruits and their seeds. This study underlines that scatter-hoarding rodents are important removers of fleshy fruits and their seeds, producing a differential seed removal depending on the seed presentation (with or without pulp), the nutritional properties of the seeds (but not of the fruits) and the presence of alternative food

Moonlight and shelter cause differential seed selection and removal by rodents

Various environmental factors may influence the foraging behaviour of seed dispersers which could ultimately affect the seed dispersal process. We examined whether moonlight levels and the presence or absence of rodentshelter affect rodentseedremoval (rate, handling time and time of removal) and seedselection (size and species) among seven oak species. The presence or absence of safe microhabitats was found to be more important than moonlight levels in the removal of seeds. Bright moonlight caused a different temporal distribution of seedremoval throughout the night but only affected the overall removal rates in open microhabitats. Seeds were removed more rapidly in open microhabitat (regardless of the moon phase), decreasing the time allocated to seed discrimination and translocation. Only in open microhabitats did increasing levels of moonlight decrease the time allocated to selection and removal of seeds. As a result, a more precise seedselection was made under shelter, owing to lower levels of predation risk. Rodent ranking preference for species was identical between full/new moon in shelter but not in open microhabitats. For all treatments, species selection by rodents was much stronger than size selection. Nevertheless, heavy seeds, which require more energy and time to be transported, were preferentially removed under shelter, where there is no time restriction to move the seeds. Our findings reveal that seedselection is safety dependent and, therefore, microhabitats in which seeds are located (sheltered versus exposed) and moonlight levels in open areas should be taken into account in rodent food selection studies.

Colocalization of α-actinin and Synaptopodin in the Pyramidal Cell Axon Initial Segment

The cisternal organelle that resides in the axon initial segment (AIS) of neocortical and hippocampal pyramidal cells is thought to be involved in regulating the Ca(2+) available to maintain AIS scaffolding proteins, thereby preserving normal AIS structure and function. Through immunocytochemistry and correlative light and electron microscopy, we show here that the actin-binding protein ?-actinin is present in the typical cistenal organelle of rodent pyramidal neurons as well as in a large structure in the AIS of a subpopulation of layer V pyramidal cells that we have called the "giant saccular organelle." Indeed, this localization of ?-actinin in the AIS is dependent on the integrity of the actin cytoskeleton. Moreover, in the cisternal organelle of cultured hippocampal neurons, ?-actinin colocalizes extensively with synaptopodin, a protein that interacts with both actin and ?-actinin, and they appear concomitantly during the development of these neurons. Together, these results indicate that ?-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS.

Identificación de los riesgos ambientales y sanitarios de la producción de gas mediante fracturación hidráulica y bases para una propuesta metodológica de vulnerabilidad de las aguas subterráneas

Hasta ahora, la gran mayoría de los recursos explotados de gas natural procedían de acumulaciones convencionales de gas aislado y de gas asociado y disuelto en el petróleo. Sin embargo, el gas natural se encuentra también en yacimientos que, debido a su baja porosidad y permeabilidad, tienen unas características que hacen que hasta muy recientemente no hayan sido económicamente rentables y que sólo puedan ser explotados mediante técnicas no convencionales, dando lugar al denominado gas no convencional. Las técnicas utilizadas para su extracción son la fracturación hidráulica o “fracking” y la perforación horizontal. Entre los diversos tipos de gas no convencional, es de prever que el gas de pizarra sea el que sufra mayor desarrollo a medio plazo en nuestro país, por lo que se están generando grandes debates, debido al riesgo de contaminación de las aguas superficiales y subterráneas del entorno, provocados por la elevada cantidad de agua utilizada, los aditivos empleados, los fluidos de retorno, por la alteración del medio físico, así como por la dificultad de monitorización de estos procesos. En este proyecto se identifican los riesgos ambientales y sanitarios asociados a la extracción de gas no convencional. El trabajo se basa principalmente en experiencias ocurridas en países donde el fracking se ha convertido en una práctica habitual. Se trata además de establecer las bases necesarias para la estimación de la vulnerabilidad de los acuíferos frente a la contaminación inducida por la fracturación hidráulica. Abstract Until now, most of the natural gas resources exploited were from isolated conventional gas accumulations and associated and dissolved gas in oil. However, the natural gas is also in reservoirs that, due to their low porosity and permeability, have characteristics that make until recently not been economically profitable and can be exploited only by unconventional techniques, leading to the so called unconventional gas. The techniques used for extraction are hydraulic fracturing or "fracking" and horizontal drilling. Among the various types of unconventional gas, it is expected that shale gas is the suffering greater medium-term development in our country, so it is generating much debate, due to the risks of contamination in surface waters and subterranean environment, caused by the high amount of water used, the additives used, the return fluid, by altering the physical environment, and the difficulty of monitoring these processes. In this project identifies the environmental and health risks associated with unconventional gas extraction. The work is mainly based on experiences that occurred in countries where fracking has become a common practice. This is for establish the necessary basis for estimating the vulnerability of aquifers from contamination induced by hydraulic fracturing.

Aplicación de la regresión logística en la predicción empírica de fenómenos complejos en obras subterráneas : squeezing y rotura de pilares de carbón

La heterogeneidad del medio geológico introduce en el proyecto de obra subterránea un alto grado de incertidumbre que debe ser debidamente gestionado a fin de reducir los riesgos asociados, que son fundamentalmente de tipo geotécnico. Entre los principales problemas a los que se enfrenta la Mecánica de Rocas moderna en el ámbito de la construcción subterránea, se encuentran la fluencia de roca en túneles (squeezing) y la rotura de pilares de carbón. Es ampliamente conocido que su aparición causa importantes perjuicios en el coste y la seguridad de los proyectos por lo que su estudio, ha estado tradicionalmente vinculado a la predicción de su ocurrencia. Entre las soluciones existentes para la determinación de estos problemas se encuentran las que se basan en métodos analíticos y numéricos. Estas metodologías son capaces de proporcionar un alto nivel de representatividad respecto del comportamiento geotécnico real, sin embargo, su utilización solo es posible cuando se dispone de una suficiente caracterización geotécnica y por tanto de una detallada definición de los parámetros que alimentan los complejos modelos constitutivos y criterios de rotura que los fenómenos estudiados requieren. Como es lógico, este nivel de definición solo es posible cuando se alcanzan etapas avanzadas de proyecto, incluso durante la propia construcción, a fin de calibrar adecuadamente los parámetros introducidos en los modelos, lo que supone una limitación de uso en etapas iniciales, cuando su predicción tiene verdadero sentido. Por su parte, los métodos empíricos permiten proporcionar soluciones a estos complejos problemas de un modo sencillo, con una baja parametrización y, dado su eminente enfoque observacional, de gran fiabilidad cuando se implementan sobre condiciones de contorno similares a las originales. La sencillez y escasez de los parámetros utilizados permiten a estas metodologías ser utilizadas desde las fases preliminares del proyecto, ya que estos constituyen en general, información habitual de fácil y económica adquisición. Este aspecto permite por tanto incorporar la predicción desde el principio del proceso de diseño, anticipando el riesgo en origen. En esta tesis doctoral, se presenta una nueva metodología empírica que sirve para proporcionar predicciones para la ocurrencia de squeezing y el fallo de pilares de carbón basada en una extensa recopilación de información de casos reales de túneles y minas en las que ambos fenómenos fueron evaluados. Esta información, recogida de referencias bibliográficas de prestigio, ha permitido recopilar una de las más extensas bases de datos existentes hasta la fecha relativa a estos fenómenos, lo que supone en sí mismo una importante contribución sobre el estado del arte. Con toda esta información, y con la ayuda de la teoría de clasificadores estadísticos, se ha implementado sobre las bases de datos un clasificador lineal de tipo regresión logística que permite hacer predicciones sobre la ocurrencia de ambos fenómenos en términos de probabilidad, y por tanto ponderar la incertidumbre asociada a la heterogeneidad incorporada por el medio geológico. Este aspecto del desarrollo es el verdadero valor añadido proporcionado por la tesis y la principal ventaja de la solución propuesta respecto de otras metodologías empíricas. Esta capacidad de ponderación probabilística permite al clasificador constituir una solución muy interesante como metodología para la evaluación de riesgo geotécnico y la toma de decisiones. De hecho, y como ejercicio de validación práctica, se ha implementado la solución desarrollada en un modelo coste-beneficio asociado a la optimización del diseño de pilares involucrados en una de mina “virtual” explotada por tajos largos. La capacidad del clasificador para cuantificar la probabilidad de fallo del diseño, junto con una adecuada cuantificación de las consecuencias de ese fallo, ha permitido definir una ley de riesgo que se ha incorporado al balance de costes y beneficios, que es capaz, a partir del redimensionamiento iterativo del sistema de pilares y de la propia configuración de la mina, maximizar el resultado económico del proyecto minero bajo unas condiciones de seguridad aceptables, fijadas de antemano. Geological media variability introduces to the subterranean project a high grade of uncertainty that should be properly managed with the aim to reduce the associated risks, which are mainly geotechnical. Among the major problems facing the modern Rock Mechanics in the field of underground construction are both, the rock squeezing while tunneling and the failure of coal pillars. Given their harmfulness to the cost and safety of the projects, their study has been traditionally linked to the determination of its occurrence. Among the existing solutions for the determination of these problems are those that are based on analytical and numerical methods. Those methodologies allow providing a high level of reliability of the geotechnical behavior, and therefore a detailed definition of the parameters that feed the complex constitutive models and failure criteria that require the studied phenomena. Obviously, this level of definition is only possible when advanced stages of the project are achieved and even during construction in order to properly calibrate the parameters entered in the models, which suppose a limited use in early stages, when the prediction has true sense. Meanwhile, empirical methods provide solutions to these complex problems in a simple way, with low parameterization and, given his observational scope, with highly reliability when implemented on similar conditions to the original context. The simplicity and scarcity of the parameters used allow these methodologies be applied in the early stages of the project, since that information should be commonly easy and cheaply to get. This aspect can therefore incorporate the prediction from the beginning of the design process, anticipating the risk beforehand. This thesis, based on the extensive data collection of case histories of tunnels and underground mines, presents a novel empirical approach used to provide predictions for the occurrence of both, squeezing and coal pillars failures. The information has been collected from prestigious references, providing one of the largest databases to date concerning phenomena, a fact which provides an important contribution to the state of the art. With all this information, and with the aid of the theory of statistical classifiers, it has been implemented on both databases, a type linear logistic regression classifier that allows predictions about the occurrence of these phenomena in terms of probability, and therefore weighting the uncertainty associated with geological variability. This aspect of the development is the real added value provided by the thesis and the main advantage of the proposed solution over other empirical methodologies. This probabilistic weighting capacity, allows being the classifier a very interesting methodology for the evaluation of geotechnical risk and decision making. In fact, in order to provide a practical validation, we have implemented the developed solution within a cost-benefit analysis associated with the optimization of the design of coal pillar systems involved in a "virtual" longwall mine. The ability of the classifier to quantify the probability of failure of the design along with proper quantification of the consequences of that failure, has allowed defining a risk law which is introduced into the cost-benefits model, which is able, from iterative resizing of the pillar system and the configuration of the mine, maximize the economic performance of the mining project under acceptable safety conditions established beforehand.