A new ultrasonic signal amplification method for detection bacteria

A new method is presented that increases the sensitivity of ultrasound-based techniques for detection of bacteria. The technique was developed for the detection of catalase-positive microorganisms. It uses a bubble trapping medium containing hydrogen peroxide that is mixed with the sample for microbiological evaluation. The enzyme catalase is present in catalase-positive bacteria, which induces a rapid hydrolysis of hydrogen peroxide, forming bubbles which remain in the medium. This reaction results in the amplification of the mechanical changes that the microorganisms produce in the medium. The effect can be detected by means of ultrasonic wave amplitude continuous measurement since the bubbles increase the ultrasonic attenuation significantly. It is shown that microorganism concentrations of the order of 105 cells ml−1 can be detected using this method. This allows an improvement of three orders of magnitude in the ultrasonic detection threshold of microorganisms in conventional culture media, and is competitive with modern rapid microbiological methods. It can also be used for the characterization of the enzymatic activity.

Dissecting Arabidopsis Gß signal transduction on the protein surface.

The heterotrimeric G-protein complex provides signal amplification and target specificity. The Arabidopsis (Arabidopsis thaliana) G?-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins. This specificity resides in the structure of the interface between AGB1 and its targets. Important surface residues of AGB1, which were deduced from a comparative evolutionary approach, were mutated to dissect AGB1-dependent physiological functions. Analysis of the capacity of these mutants to complement well-established phenotypes of G?-null mutants revealed AGB1 residues critical for specific AGB1-mediated biological processes, including growth architecture, pathogen resistance, stomata-mediated leaf-air gas exchange, and possibly photosynthesis. These findings provide promising new avenues to direct the finely tuned engineering of crop yield and traits.

Amplificador de señal analógico basado en conceptos biomiméticos. Desarrollo de modelos computacionales y validación experimental

La presente tesis doctoral estudia las características de un transductor acústico bioinspirado en la estructura del maxilar inferior de un Zifio de Couvier (Ziphius cavirostris). El mecanismo de funcionamiento del sensor se basa en las características de un sistema acoplado formado por los distintos componentes acústicos identificados en el maxilar. Para analizar las características del sensor se propone un modelo simplificado 2D que consta de una cavidad cerrada con forma de bocina acoplada a una lengüeta. Una parte de la lengüeta se encuentra dentro de la cavidad y otra en el exterior. Dicha lengüeta detecta los cambios de presión acústica y las vibraciones generadas por el sonido y las transmite con ondas de flexión al interior de la cavidad. La excitación prolongada sobre la placa puede provocar la activación de los modos propios del sistema acoplado. Dichos modos se caracterizan porque presentan un máximo de presión en el cono de la bocina la cual a su vez actúa como un amplificador acústico. Mediante el Método de los elementos Finitos se analizan las características acústicas del sensor y se construye un prototipo experimental para validar los resultados evaluados en el modelo numérico. Se propone una metodología numérica que permite desarrollar y validar un elemento tetraédrico para caracterizar el comportamiento isotrópico de los medios porosos. La metodología permite construir elementos de línea y bidimensionales. A partir de esta metodología se desarrolla un elemento plano ortotrópico. Se realiza un modelo de la cavidad en el que una de las paredes de la bocina está constituida por material poroso y se une la parte exterior de la lengüeta a dicho material para que constituya una viga sobre un apoyo elástico. Se calcula la respuesta modal y se discuten los efectos del material poroso en la eficiencia del transductor y las posibles mejoras a introducir en el mismo. SUMMARY This Thesis studies the characteristics of an acoustic transducer bioinspired by the structure of the lower maxilla of an odontoceto. In this case a Cuvier’s beaked whale (Ziphius cavirostris). The transducer working mechanism is based in a coupled system, with components identified in the maxilla. To analyze the transducer a simplified 2D model composed by a horn shaped closed cavity is modeled. The cavity is coupled with a flat belt. The belt has one part inside the cavity and the other part outside of it. The belt traverses the cavity wall and it is in charge to pick the vibrations from outside and introduce it inside the cavity. The transmission is obtained through the belt bending. A sustained external load with the right frequency contents will allow the system to reach a stationary pressure intensity distribution inside the cavity. Frequencies with modal shapes that show an important intensity increase at the horn tip are of special interest because of the signal amplification. A finite element model is constructed to study the transducer coupled modes and a prototype is constructed to validate the numerical results. A numerical methodology to construct and validate a tetrahedral finite element for isotropic porous materials is presented. The methodology allows constructing linear and 2D elements. It is extended to model orthotropic porous materials behavior. At the end, one of the horn walls is made of an orthotropic material and the external belt is glued to it in order to configure a belt over an elastic foundation. Modal response is evaluated and the porous material effects in the transducer efficiency and further improvements are discussed.