Tools in Spanish Universities to facilitate the transmission from High School to University and their assessment.

Incoming students in the University have education deficiencies, so universities studies require a sound basis of scientific knowledge. In this project are analysed instruments to reinforcing knowledge in those areas related to the studies that students are about to embark on public Spanish universities. There are important differences among universities and, in each university there are great differences among titles. Initial courses (cursos cero) are widespread (in 50% of universities) that selfevaluation instruments (14 % of universities). It is necessary to improve diffusion of those instruments because it is not possible to evaluate them. So are proposed the next actuations: to make regular standard surveys for professors and students; to publish results of surveys; public universities should institutionalize their basic training offer and improve the dissemination of this offer especially through the web. This paper presents a questionnaire to assess student opinion about these tools. To analyze the effectiveness, and make an initial estimate of the evaluation of these tools, we conducted a pilot test of the questionnaire with 68 students at the University of Extremadura. The results of preliminary statistical analysis conducted on the pilot test indicate that the survey results are reliable. A global evaluation of both tools, with a scale of 1 to 5, gave an average score of 3.29 for initial courses and 3.41 for selfevaluation. The 72.9% of the students consider the "self assessment" more effective than the "initial course"

Oscillatory genetic circuits: new designs and mathematical models

Introduction and motivation: A wide variety of organisms have developed in-ternal biomolecular clocks in order to adapt to cyclic changes of the environment. Clock operation involves genetic networks. These genetic networks have to be mod¬eled in order to understand the underlying mechanism of oscillations and to design new synthetic cellular clocks. This doctoral thesis has resulted in two contributions to the fields of genetic clocks and systems and synthetic biology, generally. The first contribution is a new genetic circuit model that exhibits an oscillatory behav¬ior through catalytic RNA molecules. The second and major contribution is a new genetic circuit model demonstrating that a repressor molecule acting on the positive feedback of a self-activating gene produces reliable oscillations. First contribution: A new model of a synthetic genetic oscillator based on a typical two-gene motif with one positive and one negative feedback loop is pre¬sented. The originality is that the repressor is a catalytic RNA molecule rather than a protein or a non-catalytic RNA molecule. This catalytic RNA is a ribozyme that acts post-transcriptionally by binding to and cleaving target mRNA molecules. This genetic clock involves just two genes, a mRNA and an activator protein, apart from the ribozyme. Parameter values that produce a circadian period in both determin¬istic and stochastic simulations have been chosen as an example of clock operation. The effects of the stochastic fluctuations are quantified by a period histogram and autocorrelation function. The conclusion is that catalytic RNA molecules can act as repressor proteins and simplify the design of genetic oscillators. Second and major contribution: It is demonstrated that a self-activating gene in conjunction with a simple negative interaction can easily produce robust matically validated. This model is comprised of two clearly distinct parts. The first is a positive feedback created by a protein that binds to the promoter of its own gene and activates the transcription. The second is a negative interaction in which a repressor molecule prevents this protein from binding to its promoter. A stochastic study shows that the system is robust to noise. A deterministic study identifies that the oscillator dynamics are mainly driven by two types of biomolecules: the protein, and the complex formed by the repressor and this protein. The main conclusion of this study is that a simple and usual negative interaction, such as degradation, se¬questration or inhibition, acting on the positive transcriptional feedback of a single gene is a sufficient condition to produce reliable oscillations. One gene is enough and the positive transcriptional feedback signal does not need to activate a second repressor gene. At the genetic level, this means that an explicit negative feedback loop is not necessary. Unlike many genetic oscillators, this model needs neither cooperative binding reactions nor the formation of protein multimers. Applications and future research directions: Recently, RNA molecules have been found to play many new catalytic roles. The first oscillatory genetic model proposed in this thesis uses ribozymes as repressor molecules. This could provide new synthetic biology design principles and a better understanding of cel¬lular clocks regulated by RNA molecules. The second genetic model proposed here involves only a repression acting on a self-activating gene and produces robust oscil¬lations. Unlike current two-gene oscillators, this model surprisingly does not require a second repressor gene. This result could help to clarify the design principles of cellular clocks and constitute a new efficient tool for engineering synthetic genetic oscillators. Possible follow-on research directions are: validate models in vivo and in vitro, research the potential of second model as a genetic memory, investigate new genetic oscillators regulated by non-coding RNAs and design a biosensor of positive feedbacks in genetic networks based on the operation of the second model Resumen Introduccion y motivacion: Una amplia variedad de organismos han desarro-llado relojes biomoleculares internos con el fin de adaptarse a los cambios ciclicos del entorno. El funcionamiento de estos relojes involucra redes geneticas. El mo delado de estas redes geneticas es esencial tanto para entender los mecanismos que producen las oscilaciones como para diseiiar nuevos circuitos sinteticos en celulas. Esta tesis doctoral ha dado lugar a dos contribuciones dentro de los campos de los circuitos geneticos en particular, y biologia de sistemas y sintetica en general. La primera contribucion es un nuevo modelo de circuito genetico que muestra un comportamiento oscilatorio usando moleculas de ARN cataliticas. La segunda y principal contribucion es un nuevo modelo de circuito genetico que demuestra que una molecula represora actuando sobre el lazo de un gen auto-activado produce oscilaciones robustas. Primera contribucion: Es un nuevo modelo de oscilador genetico sintetico basado en una tipica red genetica compuesta por dos genes con dos lazos de retroa-limentacion, uno positivo y otro negativo. La novedad de este modelo es que el represor es una molecula de ARN catalftica, en lugar de una protefna o una molecula de ARN no-catalitica. Este ARN catalitico es una ribozima que actua despues de la transcription genetica uniendose y cortando moleculas de ARN mensajero (ARNm). Este reloj genetico involucra solo dos genes, un ARNm y una proteina activadora, aparte de la ribozima. Como ejemplo de funcionamiento, se han escogido valores de los parametros que producen oscilaciones con periodo circadiano (24 horas) tanto en simulaciones deterministas como estocasticas. El efecto de las fluctuaciones es-tocasticas ha sido cuantificado mediante un histograma del periodo y la función de auto-correlacion. La conclusion es que las moleculas de ARN con propiedades cataliticas pueden jugar el misnio papel que las protemas represoras, y por lo tanto, simplificar el diseno de los osciladores geneticos. Segunda y principal contribucion: Es un nuevo modelo de oscilador genetico que demuestra que un gen auto-activado junto con una simple interaction negativa puede producir oscilaciones robustas. Este modelo ha sido estudiado y validado matematicamente. El modelo esta compuesto de dos partes bien diferenciadas. La primera parte es un lazo de retroalimentacion positiva creado por una proteina que se une al promotor de su propio gen activando la transcription. La segunda parte es una interaction negativa en la que una molecula represora evita la union de la proteina con el promotor. Un estudio estocastico muestra que el sistema es robusto al ruido. Un estudio determinista muestra que la dinamica del sistema es debida principalmente a dos tipos de biomoleculas: la proteina, y el complejo formado por el represor y esta proteina. La conclusion principal de este estudio es que una simple y usual interaction negativa, tal como una degradation, un secuestro o una inhibition, actuando sobre el lazo de retroalimentacion positiva de un solo gen es una condition suficiente para producir oscilaciones robustas. Un gen es suficiente y el lazo de retroalimentacion positiva no necesita activar a un segundo gen represor, tal y como ocurre en los relojes actuales con dos genes. Esto significa que a nivel genetico un lazo de retroalimentacion negativa no es necesario de forma explicita. Ademas, este modelo no necesita reacciones cooperativas ni la formation de multimeros proteicos, al contrario que en muchos osciladores geneticos. Aplicaciones y futuras lineas de investigacion: En los liltimos anos, se han descubierto muchas moleculas de ARN con capacidad catalitica. El primer modelo de oscilador genetico propuesto en esta tesis usa ribozimas como moleculas repre¬soras. Esto podria proporcionar nuevos principios de diseno en biologia sintetica y una mejor comprension de los relojes celulares regulados por moleculas de ARN. El segundo modelo de oscilador genetico propuesto aqui involucra solo una represion actuando sobre un gen auto-activado y produce oscilaciones robustas. Sorprendente-mente, un segundo gen represor no es necesario al contrario que en los bien conocidos osciladores con dos genes. Este resultado podria ayudar a clarificar los principios de diseno de los relojes celulares naturales y constituir una nueva y eficiente he-rramienta para crear osciladores geneticos sinteticos. Algunas de las futuras lineas de investigation abiertas tras esta tesis son: (1) la validation in vivo e in vitro de ambos modelos, (2) el estudio del potential del segundo modelo como circuito base para la construction de una memoria genetica, (3) el estudio de nuevos osciladores geneticos regulados por ARN no codificante y, por ultimo, (4) el rediseno del se¬gundo modelo de oscilador genetico para su uso como biosensor capaz de detectar genes auto-activados en redes geneticas.

Balanced scorecard to improve strategic management control at School of Mines and Energy, Universidad Politécnica de Madrid

We studied the situation in Spanish public universities regarding the use of the Balanced Scorecard (BSC), as an instrument of control and strategic management. Also, we studied its application to the School of Mines and Energy at Universidad Politécnica de Madrid. The main advantage of the BSC is that improves the organizational structure of the workplace and the achievement of the objectives that ensure long-term success. First we review the strategy for success used in the Spanish educational system and specifically in the Spanish public universities. Then using the BSC and applying the main strategic lines for the successful management of the School of Mines and Energy at Universidad Politécnica de Madrid. The strategic lines affect all the college groups and the success of the BSC tool is to increase communication between the faculties, personal auxiliary, students and society in general that make up the university. First we performed a SWOT analysis (DAFO in Spanish) there are proposed different perspectives that focus the long-term strategic objectives. The BSC is designed based on the strategic objectives that set the direction through using indicators and initiatives, the goals are achieved up to the programmed schedule. In the perspective of teaching, objectives are set to update facilities and increase partnerships with other universities and businesses, encouraging ongoing training of staff and improved coordination and internal communication. The internal process perspective aims at improving the marketing, the promotion of the international dimension of the school through strategic alliances, better mobility for students and professors and improved teaching and research quality results. It continues with improving the image of the school between customer?s perspective, the quality perceived by students and the loyalty of the teaching staff by retaining talent. Finally, the financial perspective which should contain costs without harming the quality, improving the employability of students and achieve relevant jobs at teaching and research through international measurement standards.

Emission inventories and modeling requirements for the development of air quality plans. Application to Madrid (Spain)

Modeling is an essential tool for the development of atmospheric emission abatement measures and air quality plans. Most often these plans are related to urban environments with high emission density and population exposure. However, air quality modeling in urban areas is a rather challenging task. As environmental standards become more stringent (e.g. European Directive 2008/50/EC), more reliable and sophisticated modeling tools are needed to simulate measures and plans that may effectively tackle air quality exceedances, common in large urban areas across Europe, particularly for NO2. This also implies that emission inventories must satisfy a number of conditions such as consistency across the spatial scales involved in the analysis, consistency with the emission inventories used for regulatory purposes and versatility to match the requirements of different air quality and emission projection models. This study reports the modeling activities carried out in Madrid (Spain) highlighting the atmospheric emission inventory development and preparation as an illustrative example of the combination of models and data needed to develop a consistent air quality plan at urban level. These included a series of source apportionment studies to define contributions from the international, national, regional and local sources in order to understand to what extent local authorities can enforce meaningful abatement measures. Moreover, source apportionment studies were conducted in order to define contributions from different sectors and to understand the maximum feasible air quality improvement that can be achieved by reducing emissions from those sectors, thus targeting emission reduction policies to the most relevant activities. Finally, an emission scenario reflecting the effect of such policies was developed and the associated air quality was modeled.

La planta Miralles: representación y pensamiento en la arquitectura de Enric Miralles

Resulta imposible disociar la evolución de la arquitectura de Enric Miralles de lo que fue el desarrollo de un sistema de representación propio. Partiendo de una posición heredada de su formación en la Escuela de Arquitectura de Barcelona y de su práctica en el estudio Viaplana-Piñón, donde adquiere el gusto por la precisión en el dibujo técnico, la delineación sobre papel vegetal o el grafismo constituido exclusivamente a base de líneas del mismo grosor, Miralles pronto evoluciona hacia un método caracterizado por un personal uso del sistema diédrico, vinculado a una concepción fragmentaria de la planta de arquitectura y del espacio mismo. Miralles proyectará por fragmentos de planta, asignándoles una geometría característica para diferenciarlos entre sí y desarrollar su espacialidad y sección con cierta autonomía, a través de planos y maquetas independientes. Gran parte de la arquitectura que elabora con Carme Pinós, en solitario o con Benedetta Tagliabue, estará compuesta por colecciones de piezas heterogéneas herederas de los fragmentos de la planta original, que encajan entre sí no en base a esquemas clásicos de integración subordinada o jerárquica, sino a través de posiciones relativas de yuxtaposición o superposición, caracterizadas por una ausencia de compacidad en la solución de conjunto. Este sistema de representación se apoya por tanto en la geometría como mecanismo de diferenciación por piezas, se basa en la fragmentación del diédrico desde la fragmentación de la planta, y en la falta de compacidad como soporte de pensamiento separativo. Un sistema que se define como “planta Miralles”, término que incluye todas las técnicas de representación empleadas por el arquitecto, desde planos a maquetas, pero que enfatiza la importancia estratégica de la planta como origen y guía del proyecto de arquitectura. La tesis se estructura en los tres primeros capítulos como un corolario de las categorías enunciadas, explicando, en orden cronológico a través de los proyectos, la evolución de la geometría, la utilización del diédrico, y el impacto de la falta de compacidad en la obra construida. Mientras que estos capítulos son globales, se refieren a la trayectoria de este método en su totalidad, el cuarto y último es un estudio de detalle de su aplicación en un proyecto particular, el Ayuntamiento de Utrecht, a través de los dibujos originales de Miralles. Tanto en la explicación global como en el estudio de detalle de este sistema de representación, la tesis pone de manifiesto su instrumentalidad en el pensamiento de esta arquitectura, argumentando que ésta no podría haber sido desarrollada sin la existencia del mismo. La relación entre representación y pensamiento es por tanto un tema capital para explicar esta obra. No obstante, hasta la fecha, las referencias al mismo en la bibliografía disponible no han pasado de ser una colección de opiniones dispersas, incapaces de construir por sí mismas un cuerpo estructurado y coherente de conocimiento. Se ha insistido sobremanera en el análisis y contextualización de los proyectos individuales, y poco en el estudio de la técnica proyectual utilizada para pensarlos y llevarlos a cabo. En definitiva, se han priorizado los resultados frente a los procesos creativos, existiendo por tanto un inexplicable vacío teórico respecto a un tema de gran importancia. Este vacío es el marco donde se inserta la necesidad de esta tesis doctoral. La investigación que aquí se presenta explica el origen y evolución del sistema de representación de Enric Miralles, desde su etapa como estudiante en la Escuela de Arquitectura de Barcelona hasta los últimos proyectos que elabora con Benedetta Tagliabue, así como el estudio de sus consecuencias en la obra construida. Termina concluyendo que su desarrollo es paralelo al de la arquitectura de Miralles, poniendo de manifiesto su vinculación y mutua interdependencia. ABSTRACT It is impossible to dissociate the evolution of the architecture of Enric Miralles from the development of his own system of representation. Starting from a position inherited from his training at the Barcelona School of Architecture and his practice at the office of Viaplana-Piñón, where he acquires a liking for precision in drafting and a graphic style based exclusively on lines of the same thickness, Miralles soon moves into a method defined by a customized use of the dihedral system, connected to a fragmented conception of the floorplan and space itself. Breaking up the floorplan into multiple fragments, Miralles will design an architecture where each of them has a unique shape and geometry, developing their sections and spatial qualities with a certain degree of autonomy within the whole, through separate plans and models. Many of the projects he designs with Carme Pinós, individually or with Benedetta Tagliabue, will consist of collections of heterogeneous pieces, heirs of the original floorplan fragments, which do not fit together according to classical principles of subordinate or hierarchical integration, but based on relative positions of juxtaposition or superposition that lead to a lack of compactness in the overall scheme. This system of representation is thus based on the use of geometry as a way of differentiating architectural pieces, on the fragmentation of the dihedral system from the fragmentation of the floorplan, and on a lack of compactness as a device of separative thinking. This system is defined as “Miralles plan”, a term that includes all techniques of representation used by the architect, from plans to models, and that emphasizes the particular importance of the floorplan as the guiding force of the design process. The first three chapters of the thesis have been structured as a corollary of these categories, explaining, in chronological order through Miralles’ projects, the evolution of geometry, the customization of the dihedral system, and the impact of the lack of compactness on the built work. While these three chapters are global, for they refer to the overall evolution of this system, the fourth and last one is a case study of its application to a particular project, the Utrecht Town Hall, through Miralles’ original drawings. Both in the global and particular explanations of this system of representation, the thesis highlights its instrumentality in the process of thinking this architecture, arguing that it could not have been designed without its parallel development. The relationship between thinking and representation is therefore a key issue to explain this architecture. However, to date, existing references to it in the available literature have not evolved from a collection of scattered opinions, unable to build for themselves a structured and coherent body of knowledge. Great emphasis has been put on the critical contextualization of this architecture through the analysis of the projects themselves, but little on the study of the design technique used to think and carry them out. Results have been prioritized over creative processes, existing therefore an inexplicable theoretical void on an issue of great importance. This void is the conceptual framework where the need for this thesis is inserted. This research explains the origin and evolution of Enric Miralles’ system of representation, from his time as student at the Barcelona School of Architecture to the last projects he designed with Benedetta Tagliabue, as well as the study of its impact on the built work. It concludes that the development of this system runs parallel to that of the architecture it is used for, making it explicit its indissolubility and mutual interdependence.

Energy and Lighting Performance of Buildings in the Neighbourhood Ciudad de los Angeles, Before and After Refurbishing.

The Spanish Ministry of Economy and Competitiveness is funding the SHERIF Research Project, which falls under the INNPACTO pr ogram. This project aims to increase the rate of the existing building refurbishment fro m the energy efficiency point of view by designing a facade system that must be an economica l, flexible and integrated solution 1 . Under this project has been performing several task s regarding the constructive characterization and energy evaluation of the therm al behaviour of facades on existing buildings . In order to perform the latter task, in which this article will focus, has been developing a survey of various buildings in the nei ghbourhood Ciudad de los Angeles, which has as main objective the comparison between the ac tual energy and light behaviour of different buildings, prior and posterior to any ref urbishment works have been undertaken. The evaluation of the actual performance of buildin gs before and after being refurbished is aimed to determine the impact of the work developed as well as learn from the work performed for future interventions.