Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana

Singlet oxygen is a prominent form of reactive oxygen species in higher plants. It is easily formed from molecular oxygen by triplet–triplet interchange with excited porphyrin species. Evidence has been obtained from studies on the flu mutant of Arabidopsis thaliana of a genetically determined cell death pathway that involves differential changes at the transcriptome level. Here we report on a different cell death pathway that can be deduced from the analysis of oep16 mutants of A. thaliana. Pure lines of four independent OEP16-deficient mutants with different cell death properties were isolated. Two of the mutants overproduced free protochlorophyllide (Pchlide) in the dark because of defects in import of NADPH:Pchlide oxidoreductase A (pPORA) and died after illumination. The other two mutants avoided excess Pchlide accumulation. Using pulse labeling and polysome profiling studies we show that translation is a major site of cell death regulation in flu and oep16 plants. flu plants respond to photooxidative stress triggered by singlet oxygen by reprogramming their translation toward synthesis of key enzymes involved in jasmonic acid synthesis and stress proteins. In contrast, those oep16 mutants that were prone to photooxidative damage were unable to respond in this way. Together, our results show that translation is differentially affected in the flu and oep16 mutants in response to singlet oxygen.

Los programas de regularización urbana en Brasil y su adecuación a los nuevos paradigmas urbanos tras el "Estatuto da Cidade": un estudio de casos en la ciudad de Porto Alegre, Rio Grande do Sul

En América Latina, y en Brasil en particular, las ocupaciones informales de la tierra urbana se tornaran un fenómeno generalizado en todas las ciudades, hecho que evidencio una serie de problemas urbanos y de ineficiencia en el proveimiento de los derechos básicos de los ciudadanos, principalmente el derecho a la morada digna, con eso, trajo la necesidad de priorización de política publicas curativas, como los programas de regularización urbana, cuyo objetivo es la inserción de las ocupaciones informales en la ciudad formal, con todos los impactos que eso genera: urbanísticos, legales, sociales y económicos. La ley federal intitulado Estatuto da Cidade (EC), reglamentada en 2001, es entendida como un avanzo jurídico por justamente intentar contrabalancear ese contexto, trayendo una serie de principios e instrumentos que buscan garantizar la función social de la propiedad y de la ciudad. Esa nueva lógica, en la teoría, tiene que ser la base de todas las políticas urbanas del país. Con eso, esa tesis tiene como objetivo evaluar si, realmente, los programas de regularización urbana desarrollados en Brasil cumplen con los dictámenes de dicha legislación. Para eso, fue elegido la metodología del estudio de caso, que fue desarrollado en la ciudad de Porto Alegre, capital del Rio Grande do Sul. Primero fue analizado el Estatuto da Cidade, para la definición de los principios de evaluación, luego, fue propuesto un sistema de evaluación, que fue aplicado en los dos casos estudiados: un anterior a la promulgación del EC, el Condominio dos Anjos, que serbio como parámetro referencial, y otro desarrollado tras la promulgación de dicha legislación, el Programa Integrado Entrada da Cidade (PIEC). Tras los análisis, se puede concluir que la legislación federal efectivamente no ha tenido el reflejo necesario, como conclusiones principales se puede citar: que la legislación municipal de Porto Alegre desde la década 90 ya tenía avances considerables, incluso algunos sirvieron de ejemplo en la elaboración del EC, luego, eso puede explicar el bajo impacto percibido; y que el principal fiscalizador y delineador de la política urbana es el financiador del programa, luego, muchas estrategias y dibujos proyectuales dependen de la línea de dicha financiación. ABSTRACT In Latin America, and Brazil in particular, informal urban land occupations pervasive be turned into all cities, a fact evidenced a series of urban problems and inefficiency to provide the basic rights of citizens, mainly the right to a decent housing, with that, brought the need for prioritization of public policy, such as urban regularization programs, aimed at the inclusion of informal occupations in the formal city, with all the impacts that generates: urban, legal, social and economic. Federal law entitled Estatuto da Cidade (EC), regulated in 2001, is understood as a legal advanced for just try to counterbalance this context, bringing a number of principles and instruments that seek to guarantee the social function of property and the city. This new logic, in theory, has to be the basis of all urban policies of the country. With that, this thesis aims to assess whether urban regularization programs developed in Brazil, actually, comply with the dictates of that legislation. For that, it was elected the methodology of the case study, which was developed in the city of Porto Alegre, capital of Rio Grande do Sul, Brazil. It was first analyzed the EC, for defining the principles for evaluation, then, was proposed an evaluation system, which was applied in two case studies: one before the promulgation of the EC, the Condominio dos Anjos, which used as a reference parameter, and another developed following the enactment of this legislation, the Program Integrate Entrada da Cidade (PIEC). After the analysis, it can be concluded that the federal legislation has not actually had the reflection necessary, main conclusions can be cited: the municipal legislation in Porto Alegre, since the early 90s, had considerable progress, including some served as an example in developing the EC, then, that may explain the low perceived impact; the principal auditor and eyeliner urban policy is the founder of the program, of course, many strategies and project drawings depend on the line of financing.

Water stress thresholds for regulated deficit irrigation in pistachio trees

The response of "Kerman" pistachio trees budded on three different rootstocks (Pistacia terebinthus, Pista-cia atlantica and Pistacia integerrima) to regulated deficit irrigation (RDI) in shallow soils was studied for3 years. The trees were either fully irrigated (C treatment) or subjected to deficit irrigation during Stage IIof fruit growth with two water stress thresholds (T1 and T2). The irrigation scheduling for fully-irrigatedtrees and water-stressed trees was managed by means of midday stem water potential (?stem) measure-ments. The use of direct measurements of the water status allowed estimating accurately the irrigationrequirements for pistachio trees, with water reductions ranging from 46 to 205 mm in fully-irrigatedtrees. The combination of the ?stemuse and the RDI regime saved 43?70% in T1 and 48?73% in T2 ofwater compared to the calculated crop evapotranspiration (ETc) for fully irrigated treatment (C).Deficit irrigation during Stage II significantly reduced the vegetative growth of the trees. Yield and fruitquality were not affected by any irrigation regime, except during the first year of the study. Thus, theresults indicate that full irrigation scheduling and RDI can be achieved successfully using ?stemtool onpistachio trees growing in shallow soils. A ?stemthreshold of ?1.5 MPa during stage II (T1) was suggestedfor RDI scheduling, as it did not reduce the yield or the production value. However a ?stemthresholdof ?2.0 MPa (T2) resulted in a significant reduction and an extensive delay in the recovery of stomatalconductance (gl),with negative effects on long-term pistachio production.P. integerrima showed a weaker capacity of adaptation to the study conditions compared to P. atlanticaand P. terebinthus, having a tendency to get more stressed and to produce a lower quality crop.