Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana

Singlet oxygen is a prominent form of reactive oxygen species in higher plants. It is easily formed from molecular oxygen by triplet–triplet interchange with excited porphyrin species. Evidence has been obtained from studies on the flu mutant of Arabidopsis thaliana of a genetically determined cell death pathway that involves differential changes at the transcriptome level. Here we report on a different cell death pathway that can be deduced from the analysis of oep16 mutants of A. thaliana. Pure lines of four independent OEP16-deficient mutants with different cell death properties were isolated. Two of the mutants overproduced free protochlorophyllide (Pchlide) in the dark because of defects in import of NADPH:Pchlide oxidoreductase A (pPORA) and died after illumination. The other two mutants avoided excess Pchlide accumulation. Using pulse labeling and polysome profiling studies we show that translation is a major site of cell death regulation in flu and oep16 plants. flu plants respond to photooxidative stress triggered by singlet oxygen by reprogramming their translation toward synthesis of key enzymes involved in jasmonic acid synthesis and stress proteins. In contrast, those oep16 mutants that were prone to photooxidative damage were unable to respond in this way. Together, our results show that translation is differentially affected in the flu and oep16 mutants in response to singlet oxygen.

Water stress thresholds for regulated deficit irrigation in pistachio trees

The response of "Kerman" pistachio trees budded on three different rootstocks (Pistacia terebinthus, Pista-cia atlantica and Pistacia integerrima) to regulated deficit irrigation (RDI) in shallow soils was studied for3 years. The trees were either fully irrigated (C treatment) or subjected to deficit irrigation during Stage IIof fruit growth with two water stress thresholds (T1 and T2). The irrigation scheduling for fully-irrigatedtrees and water-stressed trees was managed by means of midday stem water potential (?stem) measure-ments. The use of direct measurements of the water status allowed estimating accurately the irrigationrequirements for pistachio trees, with water reductions ranging from 46 to 205 mm in fully-irrigatedtrees. The combination of the ?stemuse and the RDI regime saved 43?70% in T1 and 48?73% in T2 ofwater compared to the calculated crop evapotranspiration (ETc) for fully irrigated treatment (C).Deficit irrigation during Stage II significantly reduced the vegetative growth of the trees. Yield and fruitquality were not affected by any irrigation regime, except during the first year of the study. Thus, theresults indicate that full irrigation scheduling and RDI can be achieved successfully using ?stemtool onpistachio trees growing in shallow soils. A ?stemthreshold of ?1.5 MPa during stage II (T1) was suggestedfor RDI scheduling, as it did not reduce the yield or the production value. However a ?stemthresholdof ?2.0 MPa (T2) resulted in a significant reduction and an extensive delay in the recovery of stomatalconductance (gl),with negative effects on long-term pistachio production.P. integerrima showed a weaker capacity of adaptation to the study conditions compared to P. atlanticaand P. terebinthus, having a tendency to get more stressed and to produce a lower quality crop.