Influence of road noise disturbances on black vulture nesting area: Case of "Cabeza de Hierro" state in Spanish Mediterranean Central Mountain

The real and potential road influence on soundscape is considered a relevant management aspect that can assess the negative effects of massive visitants on such sensitive species -that have been living for centuries in the area-. As a first approach to the study of human disturbances sound impact, acoustic engineering tools allow us to model noise pollution caused by the main road that crosses the state ?Cabeza de Hierro? (M-604). For these preliminary results we use the French method XPS 31-133, recommend at EU level. Noise emission levels in black vulture nesting area are analyzed to understand the influence of human activities on rural areas and road management on biodiversity conservation. This approach develops a useful tool to make compatible the public enjoyment of forest services such as recreation or landscape scenary, the conservation of biodiversity as well as a suitable social and economic activity level ?timber and firewood harvesting, industry?- at the region.

Germination ecology of the perennial Centaurium somedanum, a specialist species of mountain springs

To improve understanding of how a rare endemic species of Centaurium adapts to a specialized ecological niche, we studied the germination ecology of the mountain spring specialist, C. somedanum, a perennial species restricted to an unusual habitat for this genus. We conducted laboratory experiments with fresh seeds collected from two populations for three consecutive years, to investigate: (1) the effect of temperature and light ongermination; (2) the existence of seed dormancy; and (3) inter-population and inter-annual variation in germinability. Germination occurred only in the light and at relatively low temperatures (15?228C) with no differences between constant and alternating regimes, and a significant decrease at high temperatures (258C and 308C). We found non-deep simple morphophysiological dormancy and variation in seed germinability depending on the year of seed collection. C. somedanum diverged from the common germination characteristics of the genus in: (1) its germination at lower temperatures, which contrasts with what is generally expected in wetland species but could be adaptive in the spring habitat; and (2) its morphophysiological dormancy, which we report here for the first time in the genus and which could be an adaptation to its mountain habitat.

Forest Biodiversity Assessment in Peruvian Andean Montane Cloud Forest

Cloud forests are unusual and fragile habitats, being one of the least studied and least understood ecosystems. The tropical Andean dominion is considered one of the most significant places in the world as rega rds biological diversity, with a very high level of endemism. The biodiversity was analysed in an isolated remnant area of a tropical montane cloud forest known as the ?Bosque de Neblina de Cuyas?, in the North of the Peruvian Andean range. Composition, structure and dead wood were measured or estimated. The values obtained were compared with other cloud forests. The study revealed a high level of forest biodiversity, although the level of biodiversity differs from one area to another: in the inner areas, where human pressure is almost inexistent, the biodiversity values increase. The high species richness and the low dominance among species bear testimony to this montane cloud forest as a real enclave of biodiversity.

Urbanismo y sistemas agrarios periurbanos / Urbanism and periurban agrarian ecosystems

Publicación de los resultados de la primera fase del proyecto “Integración de los espacios agrarios periurbanos en la planificación urbana y territorial desde el enfoque de los servicios de los ecosistemas - PAEc-SP” (financiado por el Plan Nacional de Investigación I+D+d 2008-2012), que se presentaron en el Seminario internacional celebrado en Madrid en noviembre de 2012. Esta segunda edición, de septiembre de 2013, incorpora las modificaciones realizadas a partir de los comentarios y recomendaciones de los expertos invitados, y de los agentes territoriales a los que se presentaron los primeros resultados de la investigación.

Nitrogen deposition alters nitrogen cycling and reduces soil carbon content in low-productivity semiarid Mediterranean ecosystems.

Anthropogenic N deposition poses a threat to European Mediterranean ecosystems. We combined data from an extant N deposition gradient (4.3–7.3 kg N ha−1 yr−1) from semiarid areas of Spain and a field experiment in central Spain to evaluate N deposition effects on soil fertility, function and cyanobacteria community. Soil organic N did not increase along the extant gradient. Nitrogen fixation decreased along existing and experimental N deposition gradients, a result possibly related to compositional shifts in soil cyanobacteria community. Net ammonification and nitrification (which dominated N-mineralization) were reduced and increased, respectively, by N fertilization, suggesting alterations in the N cycle. Soil organic C content, C:N ratios and the activity of β-glucosidase decreased along the extant gradient in most locations. Our results suggest that semiarid soils in low-productivity sites are unable to store additional N inputs, and that are also unable to mitigate increasing C emissions when experiencing increased N deposition.

Habitat management for conservation of pollinators in agro-ecosystems of Central Spain

La gestión de hábitat orientada a la conservación de polinizadores en los agro-ecosistemas requiere una selección de especies vegetales atendiendo fundamentalmente a dos criterios: i) el potencial atractivo de sus flores a los polinizadores; y ii) la simplicidad en su manejo agronómico. Además de estas premisas, es necesario considerar la capacidad invasora de estas especies vegetales, debido a que algunas de las más atractivas pueden resultar invasoras en determinados agro-ecosistemas. Por lo tanto, es preciso determinar qué especies vegetales son las más indicadas para ser implementadas en cada agro-ecosistema. En la presente tesis doctoral se plantea la búsqueda de las especies vegetales adecuadas para atraer polinizadores en los agro-ecosistemas del centro de España. En una primera aproximación, se ha evaluado la atracción y expansión espacial (potencial invasivo) de seis plantas perennes de la familia Lamiaceae (aromáticas), elegidas por ser nativas de la región mediterránea. La elección de las especies vegetales se ha llevado a cabo con el fin de crear márgenes funcionales basados en la mezcla de especies vegetales con distintos periodos de floración, de modo que prolonguen la disponibilidad de recursos florales en el tiempo. Tras un primer año dedicado al establecimiento de las especies aromáticas, en los dos años siguientes se ha estudiado la atracción individual y combinada de las especies vegetales sobre los polinizadores, y como ésta se ve afectada por la densidad y la morfología floral, utilizando para ello un diseño experimental en bloques al azar. Los resultados de este estudio han puesto de manifiesto que la morfología floral no tuvo influencia sobre la atracción de las especies vegetales, pero si la densidad floral, puesto que las especies vegetales con mayor densidad de flores (Nepeta tuberosa e Hyssopus officinalis) han mostrado mayor atracción a polinizadores. Cabe destacar que de las seis especies consideradas, dos especies de verano (Melissa officinalis y Thymbra capitata) no han contribuido de forma efectiva a la atracción de la mezcla hacia los polinizadores, mostrando una reducción significativa de este parámetro respecto a las otras especies aromáticas a lo largo del verano. Se ha observado que ninguna de las especies aromáticas evaluadas ha mostrado tendencia invasora a lo largo del estudio. En base a estos resultados, se puede concluir que entre las especies aromáticas estudiadas, N. tuberosa, H. officinalis y Salvia verbenaca son las que ofrecen mayor potencial para ser utilizadas en la conservación de polinizadores. De forma similar al caso de las plantas aromáticas, se ha llevado a cabo una segunda experimentación que incluía doce plantas anuales con floración de primavera, en la que se evaluó la atracción a polinizadores y su comportamiento agronómico. Este estudio con especies herbáceas se ha prolongado durante dos años, utilizando un diseño experimental de bloques aleatorios. Las variables analizadas fueron: el atractivo de las distintas especies vegetales a los polinizadores, su eficiencia de atracción (calculada como una combinación de la duración de la floración y las visitas de insectos), su respuesta a dos tipos de manejo agronómico (cultivo en mezcla frente a monocultivo; laboreo frente a no-laboreo) y su potencial invasivo. Los resultados de esta segunda experimentación han mostrado que las flores de Borago officinalis, Echium plantagineum, Phacelia tanacetifolia y Diplotaxis tenuifolia son atractivas a las abejas, mientras que las flores de Calendula arvensis, Coriandrum sativum, D. tenuifolia y Lobularia maritima son atractivas a los sírfidos. Con independencia del tipo de polinizadores atraídos por cada especie vegetal, se ha observado una mayor eficiencia de atracción en parcelas con monocultivo de D. tenuifolia respecto a las parcelas donde se cultivó una mezcla de especies herbáceas, si bien en estas últimas se observó mayor eficiencia de atracción que en la mayoría de parcelas mono-específicas. Respecto al potencial invasivo de las especies herbáceas, a pesar de que algunas de las más atractivas a polinizadores (P. tanacetifolia and C. arvensis) mostraron tendencia a un comportamiento invasor, su capacidad de auto-reproducción se vio reducida con el laboreo. En resumen, D. tenuifolia es la única especie que presentó una alta eficiencia de atracción a distintos tipos de polinizadores, conjuntamente con una alta capacidad de auto-reproducción pero sin mostrar carácter invasor. Comparando el atractivo de las especies vegetales utilizadas en este estudio sobre los polinizadores, D. tenuifolia es la especie más recomendable para su cultivo orientado a la atracción de polinizadores en agro-ecosistemas en el centro de España. Esta especie herbácea, conocida como rúcula, tiene la ventaja añadida de ser una especie comercializada para el consumo humano. Además de su atractivo a polinizadores, deben considerarse otros aspectos relacionados con la fisiología y el comportamiento de esta especie vegetal en los agro-ecosistemas antes de recomendar su cultivo. Dado que el cultivo en un campo agrícola de una nueva especie vegetal implica unos costes de producción, por ejemplo debidos a la utilización de agua de riego, es necesario evaluar el incremento en dichos costes en función de demanda hídrica específica de esa especie vegetal. Esta variable es especialmente importante en zonas dónde se presentan sequías recurrentes como es el caso del centro y sur-este de la península Ibérica. Este razonamiento ha motivado un estudio sobre los efectos del estrés hídrico por sequía y el estrés por déficit moderado y severo de riego sobre el crecimiento y floración de la especie D. tenuifolia, así como sobre la atracción a polinizadores. Los resultados muestran que tanto el crecimiento y floración de D. tenuifolia como su atracción a polinizadores no se ven afectados si la falta de riego se produce durante un máximo de 4 días. Sin embargo, si la falta de riego se extiende a lo largo de 8 días o más, se observa una reducción significativa en el crecimiento vegetativo, el número de flores abiertas, el área total y el diámetro de dichas flores, así como en el diámetro y longitud del tubo de la corola. Por otro lado, el estudio pone de manifiesto que un déficit hídrico regulado permite una gestión eficiente del agua, la cual, dependiendo del objetivo final del cultivo de D. tenuifolia (para consumo o solo para atracción de polinizadores), puede reducir su consumo entre un 40 y un 70% sin afectar al crecimiento vegetativo y desarrollo floral, y sin reducir significativamente el atractivo a los polinizadores. Finalmente, esta tesis aborda un estudio para determinar cómo afecta el manejo de hábitat a la producción de los cultivos. En concreto, se ha planteado una experimentación que incluye márgenes mono-específicos y márgenes con una mezcla de especies atractivas a polinizadores, con el fin de determinar su efecto sobre la producción del cultivo de cilantro (C. sativum). La elección del cultivo de cilantro se debe a que requiere la polinización de insectos para su reproducción (aunque, en menor medida, puede polinizarse también por el viento), además de la facilidad para estimar su producción en condiciones semi-controladas de campo. El diseño experimental consistía en la siembra de márgenes mono-específicos de D. tenuifolia y márgenes con mezcla de seis especies anuales situados junto al cultivo de cilantro. Estos cultivos con márgenes florales fueron comparados con controles sin margen floral. Además, un segundo grupo de plantas de cilantro situadas junto a todos los tratamientos, cuyas flores fueron cubiertas para evitar su polinización, sirvió como control para evaluar la influencia de los polinizadores en la producción del cultivo. Los resultados muestran que la presencia de cualquiera de los dos tipos de margen floral mejora el peso y el porcentaje de germinación de las semillas de cilantro frente al control sin margen. Si se comparan los dos tipos de margen, se ha observado un mayor número de semillas de cilantro junto al margen con mezcla de especies florales respecto al margen mono-específico, probablemente debido al mayor número visitas de polinizadores. Puesto que el experimento se realizó en condiciones de campo semi-controladas, esto sugiere que las visitas de polinizadores fueron el factor determinante en los resultados. Por otro lado, los resultados apuntan a que la presencia de un margen floral (ya sea mono-especifico o de mezcla) en cultivos de pequeña escala puede aumentar la producción de cilantro en más de un 200%, al tiempo que contribuyen a la conservación de los polinizadores. ABSTRACT Habitat management, aimed to conserve pollinators in agro-ecosystems, requires selection of the most suitable plant species in terms of their attractiveness to pollinators and simplicity of agronomic management. However, since all flowers are not equally attractive to pollinators and many plant species can be weedy or invasive in the particular habitat, it is important to test which plant species are the most appropriate to be implemented in specific agro-ecosystems. For that reason, this PhD dissertation has been focused on determination of the most appropriate aromatic and herbaceous plants for conservation of pollinators in agro-ecosystems of Central Spain. Therefore, in a first approximation, spatial expansion (i.e. potential weediness) and attractiveness to pollinators of six aromatic perennial plants from the Lamiaceae family, native and frequent in the Mediterranean region, were evaluated. Preliminary plant selection was based on designing a functional mixed margins consisting of plants attractive to pollinators and with different blooming periods, in order to extend the availability of floral resources in the field. After a year of vegetative growth, the next two years the plant species were studied in a randomized block design experiment in order to estimate their attractiveness to pollinators in Central Spain and to investigate whether floral morphology and density affect attractiveness to pollinators. The final aim of the study was to evaluate how their phenology and attractiveness to pollinators can affect the functionality of a flowering mixture of these plants. In addition, the spatial expansion, i.e. potential weediness, of the selected plant species was estimated under field conditions, as the final purpose of the studied plants is to be implemented within agro-ecosystems. The results of the experiment showed that floral morphology did not affect the attractiveness of plants to pollinators, but floral density did, as plant species with higher floral density (i.e. Nepeta tuberosa and Hyssopus officinalis) showed significantly higher attractiveness to pollinators. In addition, of six plant species, two summer species (Melissa officinalis and Thymbra capitata) did not efficiently contribute to the attractiveness of the mixture to pollinators, which reduced its attractiveness during the summer period. Finally, as none of the plants showed weedy behaviour under field conditions, the attractive plant species, i.e. N. tuberosa, H. officinalis and the early spring flowering Salvia verbenaca, showed good potential to conserve the pollinators. Similarly, in a second approximation, the attractiveness to pollinators and agronomic behaviour of twelve herbaceous plants blooming in spring were studied. This experiment was conducted over two years in a randomized block design in order to evaluate attractiveness of preselected plant species to pollinators, as well as their attractiveness efficiency (a combination of duration of flowering and insect visitation), their response to two different agronomic management practices (growing in mixed vs. mono-specific plots; tillage vs. no-tillage), and their potential weediness. The results of this experiment showed that the flowers of Borago officinalis, Echium plantagineum, Phacelia tanacetifolia and Diplotaxis tenuifolia were attractive to bees, while Calendula arvensis, Coriandrum sativum, D. tenuifolia and Lobularia maritima were attractive to hoverflies. In addition, floral mixture resulted in lower attractiveness efficiency to pollinators than mono-specific D. tenuifolia, but higher than most of the mono-specific stands. On the other hand, although some of the most attractive plant species (e.g. P. tanacetifolia and C. arvensis) showed potential weediness, their self-seeding was reduced by tillage. After comparing attractiveness efficiency of various herbaceous species to pollinators and their potential weediness, the results indicated that D. tenuifolia showed the highest attractiveness efficiency to pollinators and efficient self-reproduction, making it highly recommended to attract bees and hoverflies in agro-ecosystems of Central Spain. In addition, this plant, commonly known as wild rocket, has a supplementary economic value as a commercialized crop. The implementation of a new floral margin in agro-ecosystems means increased production costs, especially in regions with frequent and long droughts (as it is Central and South-East area of Iberian Peninsula), where the principal agricultural cost is irrigation. Therefore, before recommending D. tenuifolia for sustainable habitat management within agro-ecosystems, it is necessary to study the effect of drought stress and moderate and severe deficit irrigation on its growth, flower development and attractiveness to pollinators. The results of this experiment showed that in greenhouse conditions, potted D. tenuifolia could be without irrigation for 4 days without affecting its growth, flowering and attractiveness to pollinators. However, lack of irrigation for 8 days or longer significantly reduced the vegetative growth, number of open flowers, total floral area, flower diameter, corolla tube diameter and corolla tube length of D. tenuifolia. This study showed that regulated deficit irrigation can improve water use efficiency, and depending on the purpose of growing D. tenuifolia, as a crop or as a beneficial plant to attract pollinators, it can reduce water consumption by 40% to 70% without affecting its vegetative and floral development and without reducing its attractiveness to pollinators. Finally, the following experiment was developed in order to understand how habitat management can influence on the agricultural production. For this purpose, it was evaluated if the vicinity of mixed and mono-specific field margins, preselected to conserve pollinators within agro-ecosystems, can improve seed production in coriander (C. sativum). The selection of this plant species for the experiment was based on its necessity for insect pollination for production of seeds (even though some pollen can be transmitted from one flower to another by wind) and the fact that under semi-controlled field conditions established in the field it is possible to estimate its total seed production. Since D. tenuifolia is attractive for both bees and hoverflies in Central Spain, the main objective of this experiment was to estimate the impact of two different types of field margins, i.e. mono-specific margin with D. tenuifolia and mixed margin with six herbaceous species, on the seed production of potted coriander. For that reason, it was tested: i) if open pollination (control without proximate field margin and treatments with nearby mono-specific and mixed margin) increases the seed production of coriander when compared with no-pollination (covered inflorescences of coriander) under field conditions; ii) if frequency of pollinator visitation to the flowers of coriander was higher in the presence of field margins than in the control without field margin; and iii) if seed production was higher in the presence of field margins than in control plants of coriander without field margin. The results showed that the proximity of both types of floral margins (mixed and mono-specific) improved the seed quality of coriander plants, as seed weight and germination rate were higher than in control plants without field margin. Furthermore, the number of seeds produced was significantly higher in coriander plants grown near mixed margins than near mono-specific margin, probably due to an increase in pollinator visits. Since the experiment was conducted under semi-controlled field conditions, it can be concluded that pollinator visits was the main factor that biased the results, and that presence of both mixed or mono-specific (D. tenuifolia) margins can improve the production of coriander for more than 200% in small-scale gardens and, in addition, conserve the local pollinators.

On the importance of shrub encroachment by sprouters, climate, species richness and anthropic factors for ecosystem multifunctionality in semi-arid Mediterranean ecosystems

One of the most important changes taking place in drylands worldwide is the increase of the cover and dominance of shrubs in areas formerly devoid of them (shrub encroachment). A large body of research has evaluated the causes and consequences of shrub encroachment for both ecosystem structure and functioning. However, there are virtually no studies evaluating how shrub encroachment affects the ability of ecosystems to maintain multiple functions and services simultaneously (multifunctionality). We aimed to do so by gathering data from ten ecosystem functions linked to the maintenance of primary production and nutrient cycling and storage (organic C, activity of β-glucosidase, pentoses, hexoses, total N, total available N, amino acids, proteins, available inorganic P, and phosphatase activity), and summarizing them in a multifunctionality index (M). We assessed how climate, species richness, anthropic factors (distance to the nearest town, sandy and asphalted road, and human population in the nearest town at several historical periods) and encroachment by sprouting shrubs impacted both the functions in isolation and M along a regional (ca. 350 km) gradient in Mediterranean grasslands and shrublands dominated by a non-sprouting shrub. Values of M were higher in those grasslands and shrublands containing sprouting shrubs (43 and 62%, respectively). A similar response was found when analyzing the different functions in isolation, as encroachment by sprouting shrubs increased functions by 2–80% compared to unencroached areas. Encroachment was the main driver of changes in M along the regional gradient evaluated, followed by anthropic factors and species richness. Climate had little effects on M in comparison to the other factors studied. Similar responses were observed when evaluating the functions in isolation. Overall, our results showed that M was higher at sites with higher sprouting shrub cover, longer distance to roads and higher perennial plant species richness. Our study is the first documenting that ecosystem multifunctionality in shrublands is enhanced by encroaching shrubs differing in size and leaf attributes. Our findings reinforce the idea that encroachment effects on ecosystem functioning cannot be generalized, and that are largely dependent on the traits of the encroaching shrub relative to those of the species being replaced.

Coleópteros Saproxílicos de los Bosques de Montaña en el Norte de la Comunidad de Madrid

La fauna saproxílica ha cobrado mucha relevancia en los últimos años. Por una parte, debido a los múltiples papeles que juega en la ecología de los bosques y por otra, por encontrarse muchas especies de ese grupo amenazadas como consecuencia de la intensificación de las actividades forestales. Se supone que los bosques de Europa meridional albergan una fauna saproxílica rica y variada. Sin embargo apenas se han realizado estudios que permitan conocer la composición de las biocenosis saproxílicas, así como el estatus y grado de amenaza a que está sometida cada especie. En esta tesis se han muestreado de forma sistemática las comunidades de coleópteros saproxílicos de cuatro montes del norte de la Comunidad de Madrid, muy diferentes a pesar de su cercanía: Dehesa Bonita de Somosierra, Hayedo de Montejo, Dehesa de Madarcos y Pinar de La Maleza. Para llevar a cabo la recogida de muestras se definió una estación de muestreo tipo, compuesta por cuatro trampas aéreas con cebo oloroso, dos trampas de ventana y una trampa de embudos. En los dos primeros montes mencionados se desplegaron seis estaciones de muestreo, por sólo tres en los otros dos. El primer objetivo de esta tesis fue conocer las especies de coleópteros que constituyen la fauna de los cuatro montes estudiados. Los muestreos sistemáticos reportaron la presencia de un total de 357 especies de coleópteros saproxílicos, siendo el Hayedo de Montejo el bosque con la diversidad más alta, 220 especies; le siguen la Dehesa de Madarcos con 116; el pinar de La Maleza con 115; y la Dehesa de Somosierra con 109, si bien la fauna de este ultimo bosque podría ser mucho más variada dado que la interferencia del ganado con algunos dispositivos de captura hizo que se perdiera parte del material allí recolectado. Se han encontrado nueve especies nuevas para la fauna de la Península Ibérica, y otras muchas desconocidas previamente en el centro peninsular. Un total de 50 especies se encuentran incluidas en la Lista Roja Europea de coleópteros saproxílicos. El segundo objetivo fue estimar la riqueza de fauna de coleópteros saproxílicos en cada bosque. Partiendo de los datos de los respectivo muestreos se calcularon diferentes estimadores, paramétricos y no paramétricos, y se elaboraron las curvas de rarefacción para cada bosque y para el conjunto. El bosque con más biodiversidad ha resultado ser el Hayedo de Montejo, que albergaría entre 254 y 332 especies. En el Pinar de la Maleza se encontrarían de 132 a 223; de 128 a 205 en la Dehesa de Somosierra; y entre 134 y 188 en la Dehesa de Madarcos. Para el conjunto del área se estimó la presencia de entre 411 y 512 especies. El tercer objetivo fue evaluar la influencia de algunos factores como la especie arbórea dominante y la cantidad de madera muerta en la riqueza y diversidad de coleópteros saproxílicos. El estudio se realizó en el Hayedo de Montejo, encontrando una alta correlación positiva entre cantidad y calidad de madera muerta, y diversidad y riqueza de especies de coleópteros saproxílicos. El cuarto objetivo fue evaluar la eficacia y complementariedad de los diferentes tipos de dispositivos de captura empleados en los muestreos. El más eficaz resultó ser la trampa de ventana, seguido por la trampa aérea con cebo oloroso, y finalmente la trampa de embudos. La mayor complementariedad se encontró entre trampas de ventana y aéreas con cebo oloroso. No obstante, si se quiere optimizar la exhaustividad del inventario no se debe prescindir de ninguno de los sistemas. En cualquier caso, puede afirmarse que la efectividad de los tres tipos de dispositivos de captura utilizados en los muestreos fue baja, pues para la gran mayoría de especies presentes se capturó un número de ejemplares realmente bajo. El bajo rendimiento de captura implica un bajo impacto sobre las poblaciones de las especies muestreadas, y esto supone una importante ventaja desde el punto de vista de la conservación. Finalmente, se dejan algunas recomendaciones de manejo a aplicar en cada uno de los montes con el fin de preservar o mejorar los hábitats utilizables por la fauna saproxílica que garanticen el mantenimiento y mejora de dichas comunidades. ABSTRACT The saproxylic fauna has become increasingly important in recent years. It has been due, on the one hand, to the multiple roles they play in the forest ecosystems and, on the other, because of the large proportion of endangered saproxylic species as a result of the intensification of forestry. It is generally assumed that southern Europe forests are home to a rich and diverse saproxylic fauna. However, there are hardly any studies leading to reveal the composition of saproxylic biocenosis, or the stage and extent of the threat each species is suffering. For the purpose of this thesis the communities of saproxylic beetles of four mountain forests in northern Comunidad de Madrid have been systematically sampled: Dehesa Bonita de Somosierra, Hayedo de Montejo, Dehesa de Madarcos and Pinar de La Maleza. They are very different from each other in spite of not being too far apart. In order to carry out sample collection, a standard sampling station was defined as follows: four smelly bait aerial traps, two window traps and one funnel trap. Six sampling stations were deployed in each of the first two forests mentioned above; put only three in each of the other two. The first aim of this thesis was to determine the composition of saproxylic beetles fauna inhabiting each of the four forests studied. Systematic sampling reported the presence of a total of 357 species of saproxylic beetles. Hayedo de Montejo, with 220 species, is the forest with the highest diversity, followed by Dehesa de Madarcos, 116; Pinar de La Maleza, 115, and Dehesa de Somosierra, 109. The fauna of the latter forest, however, could be much more varied, since cattle interference with some capture devices caused the loss of part of the material collected there. Nine new species in the fauna of the Iberian Peninsula were found, and many others previously unknown in the center of the Peninsula. A total of 41 of those species are included in the European Red List of saproxylic beetles. The second aim was to estimate the richness of saproxylic (beetle) fauna in each forest. From the data of the respective sampling, different parametric and nonparametric estimators were calculated, and rarefaction curves for each forest, as well as for the four of them together, were drawn. The most biodiverse forest turned out to be Hayedo de Montejo, which houses between 254 and 332 species. In Pinar de La Maleza, between 132 and 223 species were found; between 128 and 205 in Dehesa de Somosierra, and between 134 and 188 in Dehesa de Madarcos. The estimated diversity of species for the whole area ranges from 411 to 512. The third aim was to evaluate the influence of such factors as the dominant tree species and the amount of dead wood in the richness and diversity of saproxylic beetles. The study was conducted at Hayedo de Montejo, finding a high positive correlation between quantity and quality of coarse woody debris and diversity and richness of saproxylic beetle species. The fourth aim was to evaluate the effectiveness and complementarity of the different sampling methods used in this research work. The most effective proved to be the window trap, followed by the smelly bait aerial trap and the funnel trap, in that order. The greater complementarity was found between window and aerial traps. However, in order to optimize the completeness of the inventory, neither of the systems should be discarded. Nevertheless, the effectiveness of the three types of capture devices used in this piece of research was on the whole rather low, since for the vast majority of species, a significant low number of specimens were captured. Poor trapping performance implies a low impact on the populations of the sampled species, and this is an important advantage in terms of conservation. Finally, this thesis gives some recommendations with regard to the management of each of those four forests, leading to preserve and improve the habitats of the saproxylic wildlife and so ensure the maintenance and growth of their communities.

Global research alliance modelling platform (GRAMP): an open web platform for modelling greenhouse gas emissions from agro-ecosystems

Carbon (C) and nitrogen (N) process-based models are important tools for estimating and reporting greenhouse gas emissions and changes in soil C stocks. There is a need for continuous evaluation, development and adaptation of these models to improve scientific understanding, national inventories and assessment of mitigation options across the world. To date, much of the information needed to describe different processes like transpiration, photosynthesis, plant growth and maintenance, above and below ground carbon dynamics, decomposition and nitrogen mineralization. In ecosystem models remains inaccessible to the wider community, being stored within model computer source code, or held internally by modelling teams. Here we describe the Global Research Alliance Modelling Platform (GRAMP), a web-based modelling platform to link researchers with appropriate datasets, models and training material. It will provide access to model source code and an interactive platform for researchers to form a consensus on existing methods, and to synthesize new ideas, which will help to advance progress in this area. The platform will eventually support a variety of models, but to trial the platform and test the architecture and functionality, it was piloted with variants of the DNDC model. The intention is to form a worldwide collaborative network (a virtual laboratory) via an interactive website with access to models and best practice guidelines; appropriate datasets for testing, calibrating and evaluating models; on-line tutorials and links to modelling and data provider research groups, and their associated publications. A graphical user interface has been designed to view the model development tree and access all of the above functions.

Biogeochemical indicators of elevated nitrogen deposition in semiarid Mediterranean ecosystems

Nitrogen (N) deposition has doubled the natural N inputs received by ecosystems through biological N fixation and is currently a global problem that is affecting the Mediterranean regions. We evaluated the existing relationships between increased atmospheric N deposition and biogeochemical indicators related to soil chemical factors and cryptogam species across semiarid central, southern, and eastern Spain. The cryptogam species studied were the biocrust-forming species Pleurochaete squarrosa (moss) and Cladonia foliacea (lichen). Sampling sites were chosen in Quercus coccifera (kermes oak) shrublands and Pinus halepensis (Aleppo pine) forests to cover a range of inorganic N deposition representative of the levels found in the Iberian Peninsula (between 4.4 and 8.1 kg N ha(-1) year(-1)). We extended the ambient N deposition gradient by including experimental plots to which N had been added for 3 years at rates of 10, 20, and 50 kg N ha(-1) year(-1). Overall, N deposition (extant plus simulated) increased soil inorganic N availability and caused soil acidification. Nitrogen deposition increased phosphomonoesterase (PME) enzyme activity and PME/nitrate reductase (NR) ratio in both species, whereas the NR activity was reduced only in the moss. Responses of PME and NR activities were attributed to an induced N to phosphorus imbalance and to N saturation, respectively. When only considering the ambient N deposition, soil organic C and N contents were positively related to N deposition, a response driven by pine forests. The PME/NR ratios of the moss were better predictors of N deposition rates than PME or NR activities alone in shrublands, whereas no correlation between N deposition and the lichen physiology was observed. We conclude that integrative physiological measurements, such as PME/NR ratios, measured on sensitive species such as P. squarrosa, can provide useful data for national-scale biomonitoring programs, whereas soil acidification and soil C and N storage could be useful as additional corroborating ecosystem indicators of chronic N pollution.

Pathways towards the integration of periurban agrarian ecosystems into the spatial planning system

The potential of periurban agrarian ecosystems is recognized as one of the cornerstones for improving urban sustainability; however, this potential has been disregarded in spatial planning and decision making. The main goal of the PAEc-SP project was to assess the feasibility of integrating periurban agricultural areas into spatial planning by developing a viable ecosystem services-based methodology

Unravelling past flash flood activity in a forested mountain catchment of the Spanish Central System

Flash floods represent one of the most common natural hazards in mountain catchments, and are frequent in Mediterranean environments. As a result of the widespread lack of reliable data on past events, the understanding of their spatio-temporal occurrence and their climatic triggers remains rather limited. Here, we present a dendrogeomorphic reconstruction of past flash flood activity in the Arroyo de los Puentes stream (Sierra de Guadarrama, Spanish Central System). We analyze a total of 287 increment cores from 178 disturbed Scots pine trees (Pinus sylvestris L.) which yielded indications on 212 growth disturbances related to past flash flood impact. In combination with local archives, meteorological data, annual forest management records and highly-resolved terrestrial data (i.e., LiDAR data and aerial imagery), the dendrogeomorphic time series allowed dating 25 flash floods over the last three centuries, with a major event leaving an intense geomorphic footprint throughout the catchment in 1936. The analysis of meteorological records suggests that the rainfall thresholds of flash floods vary with the seasonality of events. Dated flash floods in the 20th century were primarily related with synoptic troughs owing to the arrival of air masses from north and west on the Iberian Peninsula during negative indices of the North Atlantic Oscillation. The results of this study contribute considerably to a better understanding of hazards related with hydrogeomorphic processes in central Spain in general and in the Sierra de Guadarrama National Park in particular.

Plant β-diversity i in human-altered forest ecosystems: the importance of the structural, spatial, and topographical characteristics of stands in patterning plant species assemblages

An understanding of spatial patterns of plant species diversity and the factors that drive those patterns is critical for the development of appropriate biodiversity management in forest ecosystems. We studied the spatial organization of plants species in human- modified and managed oak forests (primarily, Quercus faginea) in the Central Pre- Pyrenees, Spain. To test whether plant community assemblages varied non-randomly across the spatial scales, we used multiplicative diversity partitioning based on a nested hierarchical design of three increasingly coarser spatial scales (transect, stand, region). To quantify the importance of the structural, spatial, and topographical characteristics of stands in patterning plant species assemblages and identify the determinants of plant diversity patterns, we used canonical ordination. We observed a high contribution of ˟-diversity to total -diversity and found ˟-diversity to be higher and ˞-diversity to be lower than expected by random distributions of individuals at different spatial scales. Results, however, partly depended on the weighting of rare and abundant species. Variables expressing the historical management intensities of the stand such as mean stand age, the abundance of the dominant tree species (Q. faginea), age structure of the stand, and stand size were the main factors that explained the compositional variation in plant communities. The results indicate that (1) the structural, spatial, and topographical characteristics of the forest stands have the greatest effect on diversity patterns, (2) forests in landscapes that have different land use histories are environmentally heterogeneous and, therefore, can experience high levels of compositional differentiation, even at local scales (e.g., within the same stand). Maintaining habitat heterogeneity at multiple spatial scales should be considered in the development of management plans for enhancing plant diversity and related functions in human-altered forests

Towards workflow ecosystems through standard representations

Workflows are increasingly used to manage and share scientific computations and methods. Workflow tools can be used to design, validate, execute and visualize scientific workflows and their execution results. Other tools manage workflow libraries or mine their contents. There has been a lot of recent work on workflow system integration as well as common workflow interlinguas, but the interoperability among workflow systems remains a challenge. Ideally, these tools would form a workflow ecosystem such that it should be possible to create a workflow with a tool, execute it with another, visualize it with another, and use yet another tool to mine a repository of such workflows or their executions. In this paper, we describe our approach to create a workflow ecosystem through the use of standard models for provenance (OPM and W3C PROV) and extensions (P-PLAN and OPMW) to represent workflows. The ecosystem integrates different workflow tools with diverse functions (workflow generation, execution, browsing, mining, and visualization) created by a variety of research groups. This is, to our knowledge, the first time that such a variety of workflow systems and functions are integrated.

Laser remote sensing applications (LiDAR) in forest hydrology and the management of river ecosystems.

La utilización de sensores láser desde plataformas aéreas (LiDAR) ofrece nuevas posibilidades en el cartografiado de sistemas fluviales, tanto en áreas densamente cubiertas por vegetación, como en aquellas que presentan una escasa cubierta. La información topográfica de alta resolución que se obtiene a partir de las medidas láser puede ser utilizada en el análisis y estimación de diversas variables hidrológicas, y en el estudio de diferentes componentes del medio fluvial. Entre éstas, cabe citar la vegetación riparia, la morfología fluvial, el régimen hidrológico o el grado de alteración de los ecosistemas debido a las presiones de origen antrópico. La gestión del medio fluvial puede ser mejorada en gran medida gracias a la precisión y fiabilidad de esta información. En muchas ocasiones, el escaso relieve de los valles fluviales y la densa cubierta vegetal que existe en ellos han dificultado la aplicación de otras técnicas de teledetección. Sin embargo, los datos obtenidos mediante altimetría láser son especialmente aconsejables para estos trabajos, mediante análisis numéricos o a través de la simple interpretación de las imágenes obtenidas. Este artículo muestra las posibilidades de uso de los datos LiDAR en hidrología forestal y en la gestión de zonas húmedas, a lo largo de tramos con condiciones climáticas bien diferenciadas. En todas ellas, se comparan los resultados obtenidos mediante la aplicación de distinto software, con el fin de mostrar la mejor metodología de tratamiento de la información láser. Asimismo, se muestra la diferencia con otras técnicas de teledetección, y se muestra la fácil integración de los datos LiDAR con otras herramientas y metodologías de estudio de las variables citadas.