51 resultados para Losses in harvest

em Universidad Politécnica de Madrid


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The approach developed by Fuhrer in 1995 to estimate wheat yield losses induced by ozone and modulated by the soil water content (SWC) was applied to the data on Catalonian wheat yields. The aim of our work was to apply this approach and adjust it to Mediterranean environmental conditions by means of the necessary corrections. The main objective pursued was to prove the importance of soil water availability in the estimation of relative wheat yield losses as a factor that modifies the effects of tropospheric ozone on wheat, and to develop the algorithms required for the estimation of relative yield losses, adapted to the Mediterranean environmental conditions. The results show that this is an easy way to estimate relative yield losses just using meteorological data, without using ozone fluxes, which are much more difficult to calculate. Soil water availability is very important as a modulating factor of the effects of ozone on wheat; when soil water availability decreases, almost twice the amount of accumulated exposure to ozone is required to induce the same percentage of yield loss as in years when soil water availability is high.

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Field data of soiling energy losses on PV plants are scarce. Furthermore, since dirt type and accumulation vary with the location characteristics (climate, surroundings, etc.), the available data on optical losses are, necessarily, site dependent. This paper presents field measurements of dirt energy losses (dust) and irradiance incidence angle losses along 2005 on a solar-tracking PV plant located south of Navarre (Spain). The paper proposes a method to calculate these losses based on the difference between irradiance measured by calibrated cells on several trackers of the PV plant and irradiance calculated from measurements by two pyranometers (one of them incorporating a shadow ring) regularly cleaned. The equivalent optical energy losses of an installation incorporating fixed horizontal modules at the same location have been calculated as well. The effect of dirt on both types of installations will accordingly be compared.

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The propagation losses (PL) of lithium niobate optical planar waveguides fabricated by swift heavy-ion irradiation (SHI), an alternative to conventional ion implantation, have been investigated and optimized. For waveguide fabrication, congruently melting LiNbO3 substrates were irradiated with F ions at 20 MeV or 30 MeV and fluences in the range 1013–1014 cm−2. The influence of the temperature and time of post-irradiation annealing treatments has been systematically studied. Optimum propagation losses lower than 0.5 dB/cm have been obtained for both TE and TM modes, after a two-stage annealing treatment at 350 and 375∘C. Possible loss mechanisms are discussed.

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This work addresses heat losses in a CVD reactor for polysilicon production. Contributions to the energy consumption of the so-called Siemens process are evaluated, and a comprehensive model for heat loss is presented. A previously-developed model for radiative heat loss is combined with conductive heat loss theory and a new model for convective heat loss. Theoretical calculations are developed and theoretical energy consumption of the polysilicon deposition process is obtained. The model is validated by comparison with experimental results obtained using a laboratory-scale CVD reactor. Finally, the model is used to calculate heat consumption in a 36-rod industrial reactor; the energy consumption due to convective heat loss per kilogram of polysilicon produced is calculated to be 22-30 kWh/kg along a deposition process.

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This thesis contributes to the analysis and design of printed reflectarray antennas. The main part of the work is focused on the analysis of dual offset antennas comprising two reflectarray surfaces, one of them acts as sub-reflector and the second one acts as mainreflector. These configurations introduce additional complexity in several aspects respect to conventional dual offset reflectors, however they present a lot of degrees of freedom that can be used to improve the electrical performance of the antenna. The thesis is organized in four parts: the development of an analysis technique for dualreflectarray antennas, a preliminary validation of such methodology using equivalent reflector systems as reference antennas, a more rigorous validation of the software tool by manufacturing and testing a dual-reflectarray antenna demonstrator and the practical design of dual-reflectarray systems for some applications that show the potential of these kind of configurations to scan the beam and to generate contoured beams. In the first part, a general tool has been implemented to analyze high gain antennas which are constructed of two flat reflectarray structures. The classic reflectarray analysis based on MoM under local periodicity assumption is used for both sub and main reflectarrays, taking into account the incident angle on each reflectarray element. The incident field on the main reflectarray is computed taking into account the field radiated by all the elements on the sub-reflectarray.. Two approaches have been developed, one which employs a simple approximation to reduce the computer run time, and the other which does not, but offers in many cases, improved accuracy. The approximation is based on computing the reflected field on each element on the main reflectarray only once for all the fields radiated by the sub-reflectarray elements, assuming that the response will be the same because the only difference is a small variation on the angle of incidence. This approximation is very accurate when the reflectarray elements on the main reflectarray show a relatively small sensitivity to the angle of incidence. An extension of the analysis technique has been implemented to study dual-reflectarray antennas comprising a main reflectarray printed on a parabolic surface, or in general in a curved surface. In many applications of dual-reflectarray configurations, the reflectarray elements are in the near field of the feed-horn. To consider the near field radiated by the horn, the incident field on each reflectarray element is computed using a spherical mode expansion. In this region, the angles of incidence are moderately wide, and they are considered in the analysis of the reflectarray to better calculate the actual incident field on the sub-reflectarray elements. This technique increases the accuracy for the prediction of co- and cross-polar patterns and antenna gain respect to the case of using ideal feed models. In the second part, as a preliminary validation, the proposed analysis method has been used to design a dual-reflectarray antenna that emulates previous dual-reflector antennas in Ku and W-bands including a reflectarray as subreflector. The results for the dualreflectarray antenna compare very well with those of the parabolic reflector and reflectarray subreflector; radiation patterns, antenna gain and efficiency are practically the same when the main parabolic reflector is substituted by a flat reflectarray. The results show that the gain is only reduced by a few tenths of a dB as a result of the ohmic losses in the reflectarray. The phase adjustment on two surfaces provided by the dual-reflectarray configuration can be used to improve the antenna performance in some applications requiring multiple beams, beam scanning or shaped beams. Third, a very challenging dual-reflectarray antenna demonstrator has been designed, manufactured and tested for a more rigorous validation of the analysis technique presented. The proposed antenna configuration has the feed, the sub-reflectarray and the main-reflectarray in the near field one to each other, so that the conventional far field approximations are not suitable for the analysis of such antenna. This geometry is used as benchmarking for the proposed analysis tool in very stringent conditions. Some aspects of the proposed analysis technique that allow improving the accuracy of the analysis are also discussed. These improvements include a novel method to reduce the inherent cross polarization which is introduced mainly from grounded patch arrays. It has been checked that cross polarization in offset reflectarrays can be significantly reduced by properly adjusting the patch dimensions in the reflectarray in order to produce an overall cancellation of the cross-polarization. The dimensions of the patches are adjusted in order not only to provide the required phase-distribution to shape the beam, but also to exploit the crosses by zero of the cross-polarization components. The last part of the thesis deals with direct applications of the technique described. The technique presented is directly applicable to the design of contoured beam antennas for DBS applications, where the requirements of cross-polarisation are very stringent. The beam shaping is achieved by synthesithing the phase distribution on the main reflectarray while the sub-reflectarray emulates an equivalent hyperbolic subreflector. Dual-reflectarray antennas present also the ability to scan the beam over small angles about boresight. Two possible architectures for a Ku-band antenna are also described based on a dual planar reflectarray configuration that provides electronic beam scanning in a limited angular range. In the first architecture, the beam scanning is achieved by introducing a phase-control in the elements of the sub-reflectarray and the mainreflectarray is passive. A second alternative is also studied, in which the beam scanning is produced using 1-bit control on the main reflectarray, while a passive subreflectarray is designed to provide a large focal distance within a compact configuration. The system aims to develop a solution for bi-directional satellite links for emergency communications. In both proposed architectures, the objective is to provide a compact optics and simplicity to be folded and deployed.

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Recently, broadcasted 3D video content has reached households with the first generation of 3DTV. However, few studies have been done to analyze the Quality of Experience (QoE) perceived by the end-users in this scenario. This paper studies the impact of trans- mission errors in 3DTV, considering that the video is delivered in side-by-side format over a conventional packet-based network. For this purpose, a novel evaluation methodology based on standard sin- gle stimulus methods and with the aim of keeping as close as pos- sible the home environment viewing conditions has been proposed. The effects of packet losses in monoscopic and stereoscopic videos are compared from the results of subjective assessment tests. Other aspects were also measured concerning 3D content as naturalness, sense of presence and visual fatigue. The results show that although the final perceived QoE is acceptable, some errors cause important binocular rivalry, and therefore, substantial visual discomfort.

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An EMI filter for a three-phase buck-type medium power pulse-width modulation rectifier is designed. This filter considers differential mode noise and complies with MIL-STD-461E for the frequency range of 10kHz to 10MHz. In industrial applications, the frequency range of the standard starts at 150kHz and the designer typically uses a switching frequency of 28kHz because the fifth harmonic is out of the range. This approach is not valid for aircraft applications. In order to design the switching frequency in aircraft applications, the power losses in the semiconductors and the weight of the reactive components should be considered. The proposed design is based on a harmonic analysis of the rectifier input current and an analytical study of the input filter. The classical industrial design does not consider the inductive effect in the filter design because the grid frequency is 50/60Hz. However, in the aircraft applications, the grid frequency is 400Hz and the inductance cannot be neglected. The proposed design considers the inductance and the capacitance effect of the filter in order to obtain unitary power factor at full power. In the optimization process, several filters are designed for different switching frequencies of the converter. In addition, designs from single to five stages are considered. The power losses of the converter plus the EMI filter are estimated at these switching frequencies. Considering overall losses and minimal filter volume, the optimal switching frequency is selected.

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The effect of soiling in flat PV modules has been already studied, causing a reduction of the electrical output of 4% on average. For CPV's, as far as soiling produces light scattering at the optical collector surface, the scattered rays should be definitively lost because they cannot be focused onto the receivers again. While the theoretical study becomes difficult because soiling is variable at different sites, it becomes easier to begin the monitoring of the real field performance of concentrators and then raise the following question: how much does the soiling affect to PV concentrators in comparison with flat panels?? The answers allow to predict the PV concentrator electrical performance and to establish a pattern of cleaning frequency. Some experiments have been conducted at the IES-UPM and CSES-ANU sites, consisting in linear reflective concentration systems, a point focus refractive concentrator and a flat module. All the systems have been measured when soiled and then after cleaning, achieving different increases of ISC. In general, results show that CPV systems are more sensitive to soiling than flat panels, accumulating losses in ISC of about 14% on average in three different tests conducted at IESUPM and CSES-ANU test sites in Madrid (Spain) and Canberra (Australia). Some concentrators can reach losses up to 26% when the system is soiled for 4 months of exposure.

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This work studies the most beneficial way of allocating water in an irrigation community in water shortage situations. Therefore, it proposes that the irrigation surface area be divided into homogeneous zones, each with a beneficial relationship with respect to the water applied. The mathematical formula that enables one to obtain the optimal quota for the users or irrigation community as a whole has been found for individual relations of a quadratic or power type, and these have yielded different and complementary characteristics. Dimensionless variables have been used to display the results, and to compare with other alternative allocation rules such as the proportional rule, referencing the situation without water restrictions. As a result, for each water shortage situation, the water that is allocated to each user is obtained, together with the losses in individual income and the losses for the community as a whole. Furthermore, a proposal is put forth for establishing the marginal benefit from the water available, which could be of interest in enabling each community to analyze whether it is in its best interest to invest in increasing the resource, or to sell the resource to other users. Finally, an example is given to demonstrate how the method works and to show that, when the differences between the production schemes are considered, the differences in benefit reduction between the proportional allocation and the optimal allocation are also sizeable. Read More: http://ascelibrary.org/doi/abs/10.1061/(ASCE)IR.1943-4774.0000667

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Pseudomonas syringae pv tomato DC3000 (Pto) is the causal agent of the bacterial speck of tomato, which leads to significant economic losses in this crop. Pto inhabits the tomato phyllosphere, where the pathogen is highly exposed to light, among other environmental factors. Light represents a stressful condition and acts as a source of information associated with different plant defence levels. Here, we analysed the presence of both blue and red light photoreceptors in a group of Pseudomonas. In addition, we studied the effect of white, blue and red light on Pto features related to epiphytic fitness. While white and blue light inhibit motility, bacterial attachment to plant leaves is promoted. Moreover, these phenotypes are altered in a blue-light receptor mutant. These light-controlled changes during the epiphytic stage cause a reduction in virulence, highlighting the relevance of motility during the entry process to the plant apoplast. This study demonstrated the key role of light perception in the Pto phenotype switching and its effect on virulence.

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In this paper, implementation and testing of non- commercial GaN HEMT in a simple buck converter for envelope amplifier in ET and EER transmission techn iques has been done. Comparing to the prototypes with commercially available EPC1014 and 1015 GaN HEMTs, experimentally demonstrated power supply provided better thermal management and increased the switching frequency up to 25MHz. 64QAM signal with 1MHz of large signal bandw idth and 10.5dB of Peak to Average Power Ratio was gener ated, using the switching frequency of 20MHz. The obtaine defficiency was 38% including the driving circuit an d the total losses breakdown showed that switching power losses in the HEMT are the dominant ones. In addition to this, some basic physical modeling has been done, in order to provide an insight on the correlation between the electrical characteristics of the GaN HEMT and physical design parameters. This is the first step in the optimization of the HEMT design for this particular application.

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Considering a scalable video quality monitoring architecture to detect transmission errors at households, we propose a technique to detect packet losses in IPTV and Side-by-Side 3DTV and evaluate their impact on the perceived quality.

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Este trabajo es una contribución a los sistemas fotovoltaicos (FV) con seguimiento distribuido del punto de máxima potencia (DMPPT), una topología que se caracteriza porque lleva a cabo el MPPT a nivel de módulo, al contrario de las topologías más tradicionales que llevan a cabo el MPPT para un número más elevado de módulos, pudiendo ser hasta cientos de módulos. Las dos tecnologías DMPPT que existen en el mercado son conocidos como microinversores y optimizadores de potencia, y ofrecen ciertas ventajas sobre sistemas de MPPT central como: mayor producción en situaciones de mismatch, monitorización individual de cada módulo, flexibilidad de diseño, mayor seguridad del sistema, etc. Aunque los sistemas DMPPT no están limitados a los entornos urbanos, se ha enfatizado en el título ya que es su mercado natural, siendo difícil una justificación de su sobrecoste en grandes huertas solares en suelo. Desde el año 2010 el mercado de estos sistemas ha incrementado notablemente y sigue creciendo de una forma continuada. Sin embargo, todavía falta un conocimiento profundo de cómo funcionan estos sistemas, especialmente en el caso de los optimizadores de potencia, de las ganancias energéticas esperables en condiciones de mismatch y de las posibilidades avanzadas de diagnóstico de fallos. El principal objetivo de esta tesis es presentar un estudio completo de cómo funcionan los sistemas DMPPT, sus límites y sus ventajas, así como experimentos varios que verifican la teoría y el desarrollo de herramientas para valorar las ventajas de utilizar DMPPT en cada instalación. Las ecuaciones que modelan el funcionamiento de los sistemas FVs con optimizadores de potencia se han desarrollado y utilizado para resaltar los límites de los mismos a la hora de resolver ciertas situaciones de mismatch. Se presenta un estudio profundo sobre el efecto de las sombras en los sistemas FVs: en la curva I-V y en los algoritmos MPPT. Se han llevado a cabo experimentos sobre el funcionamiento de los algoritmos MPPT en situaciones de sombreado, señalando su ineficiencia en estas situaciones. Un análisis de la ventaja del uso de DMPPT frente a los puntos calientes es presentado y verificado. También se presenta un análisis sobre las posibles ganancias en potencia y energía con el uso de DMPPT en condiciones de sombreado y este también es verificado experimentalmente, así como un breve estudio de su viabilidad económica. Para ayudar a llevar a cabo todos los análisis y experimentos descritos previamente se han desarrollado una serie de herramientas software. Una siendo un programa en LabView para controlar un simulador solar y almacenar las medidas. También se ha desarrollado un programa que simula curvas I-V de módulos y generador FVs afectados por sombras y este se ha verificado experimentalmente. Este mismo programa se ha utilizado para desarrollar un programa todavía más completo que estima las pérdidas anuales y las ganancias obtenidas con DMPPT en instalaciones FVs afectadas por sombras. Finalmente, se han desarrollado y verificado unos algoritmos para diagnosticar fallos en sistemas FVs con DMPPT. Esta herramienta puede diagnosticar los siguientes fallos: sombras debido a objetos fijos (con estimación de la distancia al objeto), suciedad localizada, suciedad general, posible punto caliente, degradación de módulos y pérdidas en el cableado de DC. Además, alerta al usuario de las pérdidas producidas por cada fallo y no requiere del uso de sensores de irradiancia y temperatura. ABSTRACT This work is a contribution to photovoltaic (PV) systems with distributed maximum power point tracking (DMPPT), a system topology characterized by performing the MPPT at module level, instead of the more traditional topologies which perform MPPT for a larger number of modules. The two DMPPT technologies available at the moment are known as microinverters and power optimizers, also known as module level power electronics (MLPE), and they provide certain advantages over central MPPT systems like: higher energy production in mismatch situations, monitoring of each individual module, system design flexibility, higher system safety, etc. Although DMPPT is not limited to urban environments, it has been emphasized in the title as it is their natural market, since in large ground-mounted PV plants the extra cost is difficult to justify. Since 2010 MLPE have increased their market share steadily and continuing to grow steadily. However, there still lacks a profound understanding of how they work, especially in the case of power optimizers, the achievable energy gains with their use and the possibilities in failure diagnosis. The main objective of this thesis is to provide a complete understanding of DMPPT technologies: how they function, their limitations and their advantages. A series of equations used to model PV arrays with power optimizers have been derived and used to point out limitations in solving certain mismatch situation. Because one of the most emphasized benefits of DMPPT is their ability to mitigate shading losses, an extensive study on the effects of shadows on PV systems is presented; both on the I-V curve and on MPPT algorithms. Experimental tests have been performed on the MPPT algorithms of central inverters and MLPE, highlighting their inefficiency in I-V curves with local maxima. An analysis of the possible mitigation of hot-spots with DMPPT is discussed and experimentally verified. And a theoretical analysis of the possible power and energy gains is presented as well as experiments in real PV systems. A short economic analysis of the benefits of DMPPT has also been performed. In order to aide in the previous task, a program which simulates I-V curves under shaded conditions has been developed and experimentally verified. This same program has been used to develop a software tool especially designed for PV systems affected by shading, which estimates the losses due to shading and the energy gains obtained with DMPPT. Finally, a set of algorithms for diagnosing system faults in PV systems with DMPPT has been developed and experimentally verified. The tool can diagnose the following failures: fixed object shading (with distance estimation), localized dirt, generalized dirt, possible hot-spots, module degradation and excessive losses in DC cables. In addition, it alerts the user of the power losses produced by each failure and classifies the failures by their severity and it does not require the use of irradiance or temperature sensors.

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Este trabajo es una contribución a los sistemas fotovoltaicos (FV) con seguimiento distribuido del punto de máxima potencia (DMPPT), una topología que se caracteriza porque lleva a cabo el MPPT a nivel de módulo, al contrario de las topologías más tradicionales que llevan a cabo el MPPT para un número más elevado de módulos, pudiendo ser hasta cientos de módulos. Las dos tecnologías DMPPT que existen en el mercado son conocidos como microinversores y optimizadores de potencia, y ofrecen ciertas ventajas sobre sistemas de MPPT central como: mayor producción en situaciones de mismatch, monitorización individual de cada módulo, flexibilidad de diseño, mayor seguridad del sistema, etc. Aunque los sistemas DMPPT no están limitados a los entornos urbanos, se ha enfatizado en el título ya que es su mercado natural, siendo difícil una justificación de su sobrecoste en grandes huertas solares en suelo. Desde el año 2010 el mercado de estos sistemas ha incrementado notablemente y sigue creciendo de una forma continuada. Sin embargo, todavía falta un conocimiento profundo de cómo funcionan estos sistemas, especialmente en el caso de los optimizadores de potencia, de las ganancias energéticas esperables en condiciones de mismatch y de las posibilidades avanzadas de diagnóstico de fallos. El principal objetivo de esta tesis es presentar un estudio completo de cómo funcionan los sistemas DMPPT, sus límites y sus ventajas, así como experimentos varios que verifican la teoría y el desarrollo de herramientas para valorar las ventajas de utilizar DMPPT en cada instalación. Las ecuaciones que modelan el funcionamiento de los sistemas FVs con optimizadores de potencia se han desarrollado y utilizado para resaltar los límites de los mismos a la hora de resolver ciertas situaciones de mismatch. Se presenta un estudio profundo sobre el efecto de las sombras en los sistemas FVs: en la curva I-V y en los algoritmos MPPT. Se han llevado a cabo experimentos sobre el funcionamiento de los algoritmos MPPT en situaciones de sombreado, señalando su ineficiencia en estas situaciones. Un análisis de la ventaja del uso de DMPPT frente a los puntos calientes es presentado y verificado. También se presenta un análisis sobre las posibles ganancias en potencia y energía con el uso de DMPPT en condiciones de sombreado y este también es verificado experimentalmente, así como un breve estudio de su viabilidad económica. Para ayudar a llevar a cabo todos los análisis y experimentos descritos previamente se han desarrollado una serie de herramientas software. Una siendo un programa en LabView para controlar un simulador solar y almacenar las medidas. También se ha desarrollado un programa que simula curvas I-V de módulos y generador FVs afectados por sombras y este se ha verificado experimentalmente. Este mismo programa se ha utilizado para desarrollar un programa todavía más completo que estima las pérdidas anuales y las ganancias obtenidas con DMPPT en instalaciones FVs afectadas por sombras. Finalmente, se han desarrollado y verificado unos algoritmos para diagnosticar fallos en sistemas FVs con DMPPT. Esta herramienta puede diagnosticar los siguientes fallos: sombras debido a objetos fijos (con estimación de la distancia al objeto), suciedad localizada, suciedad general, posible punto caliente, degradación de módulos y pérdidas en el cableado de DC. Además, alerta al usuario de las pérdidas producidas por cada fallo y no requiere del uso de sensores de irradiancia y temperatura. ABSTRACT This work is a contribution to photovoltaic (PV) systems with distributed maximum power point tracking (DMPPT), a system topology characterized by performing the MPPT at module level, instead of the more traditional topologies which perform MPPT for a larger number of modules. The two DMPPT technologies available at the moment are known as microinverters and power optimizers, also known as module level power electronics (MLPE), and they provide certain advantages over central MPPT systems like: higher energy production in mismatch situations, monitoring of each individual module, system design flexibility, higher system safety, etc. Although DMPPT is not limited to urban environments, it has been emphasized in the title as it is their natural market, since in large ground-mounted PV plants the extra cost is difficult to justify. Since 2010 MLPE have increased their market share steadily and continuing to grow steadily. However, there still lacks a profound understanding of how they work, especially in the case of power optimizers, the achievable energy gains with their use and the possibilities in failure diagnosis. The main objective of this thesis is to provide a complete understanding of DMPPT technologies: how they function, their limitations and their advantages. A series of equations used to model PV arrays with power optimizers have been derived and used to point out limitations in solving certain mismatch situation. Because one of the most emphasized benefits of DMPPT is their ability to mitigate shading losses, an extensive study on the effects of shadows on PV systems is presented; both on the I-V curve and on MPPT algorithms. Experimental tests have been performed on the MPPT algorithms of central inverters and MLPE, highlighting their inefficiency in I-V curves with local maxima. An analysis of the possible mitigation of hot-spots with DMPPT is discussed and experimentally verified. And a theoretical analysis of the possible power and energy gains is presented as well as experiments in real PV systems. A short economic analysis of the benefits of DMPPT has also been performed. In order to aide in the previous task, a program which simulates I-V curves under shaded conditions has been developed and experimentally verified. This same program has been used to develop a software tool especially designed for PV systems affected by shading, which estimates the losses due to shading and the energy gains obtained with DMPPT. Finally, a set of algorithms for diagnosing system faults in PV systems with DMPPT has been developed and experimentally verified. The tool can diagnose the following failures: fixed object shading (with distance estimation), localized dirt, generalized dirt, possible hot-spots, module degradation and excessive losses in DC cables. In addition, it alerts the user of the power losses produced by each failure and classifies the failures by their severity and it does not require the use of irradiance or temperature sensors.

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Actualmente, la gestión de sistemas de Manejo Integrado de Plagas (MIP) en cultivos hortícolas tiene por objetivo priorizar los métodos de control no químicos en detrimento del consumo de plaguicidas, según recoge la directiva europea 2009/128/CE ‘Uso Sostenible de Plaguicidas’ (OJEC, 2009). El uso de agentes de biocontrol como alternativa a la aplicación de insecticidas es un elemento clave de los sistemas MIP por sus innegables ventajas ambientales que se utiliza ampliamente en nuestro país (Jacas y Urbaneja, 2008). En la región de Almería, donde se concentra el 65% de cultivo en invernadero de nuestro país (47.367 ha), MIP es la principal estrategia en pimiento (MAGRAMA, 2014), y comienza a serlo en otros cultivos como tomate o pepino. El cultivo de pepino, con 8.902 ha (MAGRAMA, 2013), tiene un protocolo semejante al pimiento (Robledo et al., 2009), donde la única especie de pulgón importante es Aphis gossypii Glover. Sin embargo, pese al continuo incremento de la superficie de cultivo agrícola bajo sistemas MIP, los daños originados por virosis siguen siendo notables. Algunos de los insectos presentes en los cultivos de hortícolas son importantes vectores de virus, como los pulgones, las moscas blancas o los trips, cuyo control resulta problemático debido a su elevada capacidad para transmitir virus vegetales incluso a una baja densidad de plaga (Holt et al., 2008; Jacas y Urbaneja, 2008). Las relaciones que se establecen entre los distintos agentes de un ecosistema son complejas y muy específicas. Se ha comprobado que, pese a que los enemigos naturales reducen de manera beneficiosa los niveles de plaga, su incorporación en los sistemas planta-insecto-virus puede desencadenar complicadas interacciones con efectos no deseables (Dicke y van Loon, 2000; Jeger et al., 2011). Así, los agentes de biocontrol también pueden inducir a que los insectos vectores modifiquen su comportamiento como respuesta al ataque y, con ello, el grado de dispersión y los patrones de distribución de las virosis que transmiten (Bailey et al., 1995; Weber et al., 1996; Hodge y Powell, 2008a; Hodge et al., 2011). Además, en ocasiones el control biológico por sí solo no es suficiente para controlar determinadas plagas (Medina et al., 2008). Entre los métodos que se pueden aplicar bajo sistemas MIP están las barreras físicas que limitan la entrada de plagas al interior de los invernaderos o interfieren con su movimiento, como pueden ser las mallas anti-insecto (Álvarez et al., 2014), las mallas fotoselectivas (Raviv y Antignus, 2004; Weintraub y Berlinger, 2004; Díaz y Fereres, 2007) y las mallas impregnadas en insecticida (Licciardi et al., 2008; Martin et al., 2014). Las mallas fotoselectivas reducen o bloquean casi por completo la transmisión de radiación UV, lo que interfiere con la visión de los insectos y dificulta o impide la localización del cultivo y su establecimiento en el mismo (Raviv y Antignus, 2004; Weintraub, 2009). Se ha comprobado cómo su uso puede controlar los pulgones y las virosis en cultivo de lechuga (Díaz et al., 2006; Legarrea et al., 2012a), así como la mosca blanca, los trips y los ácaros, y los virus que estos transmiten en otros cultivos (Costa y Robb, 1999; Antignus et al., 2001; Kumar y Poehling, 2006; Doukas y Payne, 2007a; Legarrea et al., 2010). Sin embargo, no se conoce perfectamente el modo de acción de estas barreras, puesto que existe un efecto directo sobre la plaga y otro indirecto mediado por la planta, cuya fisiología cambia al desarrollarse en ambientes con falta de radiación UV, y que podría afectar al ciclo biológico de los insectos fitófagos (Vänninen et al., 2010; Johansen et al., 2011). Del mismo modo, es necesario estudiar la compatibilidad de esta estrategia con los enemigos naturales de las plagas. Hasta la fecha, los estudios han evidenciado que los agentes de biocontrol pueden realizar su actividad bajo ambientes pobres en radiación UV (Chyzik et al., 2003; Chiel et al., 2006; Doukas y Payne, 2007b; Legarrea et al., 2012c). Otro método basado en barreras físicas son las mallas impregnadas con insecticidas, que se han usado tradicionalmente en la prevención de enfermedades humanas transmitidas por mosquitos (Martin et al., 2006). Su aplicación se ha ensayado en agricultura en ciertos cultivos al aire libre (Martin et al., 2010; Díaz et al., 2004), pero su utilidad en cultivos protegidos para prevenir la entrada de insectos vectores en invernadero todavía no ha sido investigada. Los aditivos se incorporan al tejido durante el proceso de extrusión de la fibra y se liberan lentamente actuando por contacto en el momento en que el insecto aterriza sobre la malla, con lo cual el riesgo medioambiental y para la salud humana es muy limitado. Los plaguicidas que se emplean habitualmente suelen ser piretroides (deltametrina o bifentrín), aunque también se ha ensayado dicofol (Martin et al., 2010) y alfa-cipermetrina (Martin et al., 2014). Un factor que resulta de vital importancia en este tipo de mallas es el tamaño del poro para facilitar una buena ventilación del cultivo, al tiempo que se evita la entrada de insectos de pequeño tamaño como las moscas blancas (Bethke y Paine, 1991; Muñoz et al., 1999). Asimismo, se plantea la necesidad de estudiar la compatibilidad de estas mallas con los enemigos naturales. Es por ello que en esta Tesis Doctoral se plantea la necesidad de evaluar nuevas mallas impregnadas que impidan el paso de insectos de pequeño tamaño al interior de los invernaderos, pero que a su vez mantengan un buen intercambio y circulación de aire a través del poro de la malla. Así, en la presente Tesis Doctoral, se han planteado los siguientes objetivos generales a desarrollar: 1. Estudiar el impacto de la presencia de parasitoides sobre el grado de dispersión y los patrones de distribución de pulgones y las virosis que éstos transmiten. 2. Conocer el efecto directo de ambientes pobres en radiación UV sobre el comportamiento de vuelo de plagas clave de hortícolas y sus enemigos naturales. 3. Evaluar el efecto directo de la radiación UV-A sobre el crecimiento poblacional de pulgones y mosca blanca, y sobre la fisiología de sus plantas hospederas, así como el efecto indirecto de la radiación UV-A en ambas plagas mediado por el crecimiento de dichas planta hospederas. 4. Caracterización de diversas mallas impregnadas en deltametrina y bifentrín con diferentes propiedades y selección de las óptimas para el control de pulgones, mosca blanca y sus virosis asociadas en condiciones de campo. Estudio de su compatibilidad con parasitoides. ABSTRACT Insect vectors of plant viruses are the main agents causing major economic losses in vegetable crops grown under protected environments. This Thesis focuses on the implementation of new alternatives to chemical control of insect vectors under Integrated Pest Management programs. In Spain, biological control is the main pest control strategy used in a large part of greenhouses where horticultural crops are grown. The first study aimed to increase our knowledge on how the presence of natural enemies such as Aphidius colemani Viereck may alter the dispersal of the aphid vector Aphis gossypii Glover (Chapter 4). In addition, it was investigated if the presence of this parasitoid affected the spread of aphid-transmitted viruses Cucumber mosaic virus (CMV, Cucumovirus) and Cucurbit aphid-borne yellows virus (CABYV, Polerovirus) infecting cucumber (Cucumis sativus L). SADIE methodology was used to study the distribution patterns of both the virus and its vector, and their degree of association. Results suggested that parasitoids promoted aphid dispersal in the short term, which enhanced CMV spread, though consequences of parasitism suggested potential benefits for disease control in the long term. Furthermore, A. colemani significantly limited the spread and incidence of the persistent virus CABYV in the long term. The flight activity of pests Myzus persicae (Sulzer), Bemisia tabaci (Gennadius) and Tuta absoluta (Meyrick), and natural enemies A. colemani and Sphaerophoria rueppellii (Weidemann) under UV-deficient environments was studied under field conditions (Chapter 5). One-chamber tunnels were covered with cladding materials with different UV transmittance properties. Inside each tunnel, insects were released from tubes placed in a platform suspended from the ceiling. Specific targets were located at different distances from the platform. The ability of aphids and whiteflies to reach their targets was diminished under UV-absorbing barriers, suggesting a reduction of vector activity under this type of nets. Fewer aphids reached distant traps under UV-absorbing nets, and significantly more aphids could fly to the end of the tunnels covered with non-UV blocking materials. Unlike aphids, differences in B. tabaci captures were mainly found in the closest targets. The oviposition of lepidopteran T. absoluta was also negatively affected by a UV-absorbing cover. The photoselective barriers were compatible with parasitism and oviposition of biocontrol agents. Apart from the direct response of insects to UV radiation, plant-mediated effects influencing insect performance were investigated (Chapter 6). The impact of UV-A radiation on the performance of aphid M. persicae and whitefly B. tabaci, and growth and leaf physiology of host plants pepper and eggplant was studied under glasshouse conditions. Plants were grown inside cages covered by transparent and UV-A-opaque plastic films. Plant growth and insect fitness were monitored. Leaves were harvested for chemical analysis. Pepper plants responded directly to UV-A by producing shorter stems whilst UV-A did not affect the leaf area of either species. UV-A-treated peppers had higher content of secondary metabolites, soluble carbohydrates, free amino acids and proteins. Such changes in tissue chemistry indirectly promoted aphid performance. For eggplants, chlorophyll and carotenoid levels decreased with supplemental UVA but phenolics were not affected. Exposure to supplemental UV-A had a detrimental effect on whitefly development, fecundity and fertility presumably not mediated by plant cues, as compounds implied in pest nutrition were unaltered. Lastly, the efficacy of a wide range of Long Lasting Insecticide Treated Nets (LLITNs) was studied under laboratory and field conditions. This strategy aimed to prevent aphids and whiteflies to enter the greenhouse by determining the optimum mesh size (Chapter 7). This new approach is based on slow release deltamethrin- and bifenthrin-treated nets with large hole sizes that allow improved ventilation of greenhouses. All LLITNs produced high mortality of M. persicae and A. gossypii although their efficacy decreased over time with sun exposure. It was necessary a net with hole size of 0.29 mm2 to exclude B. tabaci under laboratory conditions. The feasibility of two selected nets was studied in the field under a high insect infestation pressure in the presence of CMV- and CABYV-infected cucumber plants. Besides, the compatibility of parasitoid A. colemani with bifenthrin-treated nets was studied in parallel field experiments. Both nets effectively blocked the invasion of aphids and reduced the incidence of both viruses, however they failed to exclude whiteflies. We found that our LLITNs were compatible with parasitoid A. colemani. As shown, the role of natural enemies has to be taken into account regarding the dispersal of insect vectors and subsequent spread of plant viruses. The additional benefits of novel physicochemical barriers, such as photoselective and insecticide-impregnated nets, need to be considered in Integrated Pest Management programs of vegetable crops grown under protected environments.