Microarray analysis of autoimmune diseases by machine learning procedures

—Microarray-based global gene expression profiling, with the use of sophisticated statistical algorithms is providing new insights into the pathogenesis of autoimmune diseases. We have applied a novel statistical technique for gene selection based on machine learning approaches to analyze microarray expression data gathered from patients with systemic lupus erythematosus (SLE) and primary antiphospholipid syndrome (PAPS), two autoimmune diseases of unknown genetic origin that share many common features. The methodology included a combination of three data discretization policies, a consensus gene selection method, and a multivariate correlation measurement. A set of 150 genes was found to discriminate SLE and PAPS patients from healthy individuals. Statistical validations demonstrate the relevance of this gene set from an univariate and multivariate perspective. Moreover, functional characterization of these genes identified an interferon-regulated gene signature, consistent with previous reports. It also revealed the existence of other regulatory pathways, including those regulated by PTEN, TNF, and BCL-2, which are altered in SLE and PAPS. Remarkably, a significant number of these genes carry E2F binding motifs in their promoters, projecting a role for E2F in the regulation of autoimmunity.

Clasificación de tráfico de Internet mediante análisis de datos

Poder clasificar de manera precisa la aplicación o programa del que provienen los flujos que conforman el tráfico de uso de Internet dentro de una red permite tanto a empresas como a organismos una útil herramienta de gestión de los recursos de sus redes, así como la posibilidad de establecer políticas de prohibición o priorización de tráfico específico. La proliferación de nuevas aplicaciones y de nuevas técnicas han dificultado el uso de valores conocidos (well-known) en puertos de aplicaciones proporcionados por la IANA (Internet Assigned Numbers Authority) para la detección de dichas aplicaciones. Las redes P2P (Peer to Peer), el uso de puertos no conocidos o aleatorios, y el enmascaramiento de tráfico de muchas aplicaciones en tráfico HTTP y HTTPS con el fin de atravesar firewalls y NATs (Network Address Translation), entre otros, crea la necesidad de nuevos métodos de detección de tráfico. El objetivo de este estudio es desarrollar una serie de prácticas que permitan realizar dicha tarea a través de técnicas que están más allá de la observación de puertos y otros valores conocidos. Existen una serie de metodologías como Deep Packet Inspection (DPI) que se basa en la búsqueda de firmas, signatures, en base a patrones creados por el contenido de los paquetes, incluido el payload, que caracterizan cada aplicación. Otras basadas en el aprendizaje automático de parámetros de los flujos, Machine Learning, que permite determinar mediante análisis estadísticos a qué aplicación pueden pertenecer dichos flujos y, por último, técnicas de carácter más heurístico basadas en la intuición o el conocimiento propio sobre tráfico de red. En concreto, se propone el uso de alguna de las técnicas anteriormente comentadas en conjunto con técnicas de minería de datos como son el Análisis de Componentes Principales (PCA por sus siglas en inglés) y Clustering de estadísticos extraídos de los flujos procedentes de ficheros de tráfico de red. Esto implicará la configuración de diversos parámetros que precisarán de un proceso iterativo de prueba y error que permita dar con una clasificación del tráfico fiable. El resultado ideal sería aquel en el que se pudiera identificar cada aplicación presente en el tráfico en un clúster distinto, o en clusters que agrupen grupos de aplicaciones de similar naturaleza. Para ello, se crearán capturas de tráfico dentro de un entorno controlado e identificando cada tráfico con su aplicación correspondiente, a continuación se extraerán los flujos de dichas capturas. Tras esto, parámetros determinados de los paquetes pertenecientes a dichos flujos serán obtenidos, como por ejemplo la fecha y hora de llagada o la longitud en octetos del paquete IP. Estos parámetros serán cargados en una base de datos MySQL y serán usados para obtener estadísticos que ayuden, en un siguiente paso, a realizar una clasificación de los flujos mediante minería de datos. Concretamente, se usarán las técnicas de PCA y clustering haciendo uso del software RapidMiner. Por último, los resultados obtenidos serán plasmados en una matriz de confusión que nos permitirá que sean valorados correctamente. ABSTRACT. Being able to classify the applications that generate the traffic flows in an Internet network allows companies and organisms to implement efficient resource management policies such as prohibition of specific applications or prioritization of certain application traffic, looking for an optimization of the available bandwidth. The proliferation of new applications and new technics in the last years has made it more difficult to use well-known values assigned by the IANA (Internet Assigned Numbers Authority), like UDP and TCP ports, to identify the traffic. Also, P2P networks and data encapsulation over HTTP and HTTPS traffic has increased the necessity to improve these traffic analysis technics. The aim of this project is to develop a number of techniques that make us able to classify the traffic with more than the simple observation of the well-known ports. There are some proposals that have been created to cover this necessity; Deep Packet Inspection (DPI) tries to find signatures in the packets reading the information contained in them, the payload, looking for patterns that can be used to characterize the applications to which that traffic belongs; Machine Learning procedures work with statistical analysis of the flows, trying to generate an automatic process that learns from those statistical parameters and calculate the likelihood of a flow pertaining to a certain application; Heuristic Techniques, finally, are based in the intuition or the knowledge of the researcher himself about the traffic being analyzed that can help him to characterize the traffic. Specifically, the use of some of the techniques previously mentioned in combination with data mining technics such as Principal Component Analysis (PCA) and Clustering (grouping) of the flows extracted from network traffic captures are proposed. An iterative process based in success and failure will be needed to configure these data mining techniques looking for a reliable traffic classification. The perfect result would be the one in which the traffic flows of each application is grouped correctly in each cluster or in clusters that contain group of applications of similar nature. To do this, network traffic captures will be created in a controlled environment in which every capture is classified and known to pertain to a specific application. Then, for each capture, all the flows will be extracted. These flows will be used to extract from them information such as date and arrival time or the IP length of the packets inside them. This information will be then loaded to a MySQL database where all the packets defining a flow will be classified and also, each flow will be assigned to its specific application. All the information obtained from the packets will be used to generate statistical parameters in order to describe each flow in the best possible way. After that, data mining techniques previously mentioned (PCA and Clustering) will be used on these parameters making use of the software RapidMiner. Finally, the results obtained from the data mining will be compared with the real classification of the flows that can be obtained from the database. A Confusion Matrix will be used for the comparison, letting us measure the veracity of the developed classification process.

Interactive Graphic Simulation: An Advanced Methodology to Improve the Teaching-Learning Process in Nuclear Engineering Education and Training

Nowadays, computer simulators are becoming basic tools for education and training in many engineering fields. In the nuclear industry, the role of simulation for training of operators of nuclear power plants is also recognized of the utmost relevance. As an example, the International Atomic Energy Agency sponsors the development of nuclear reactor simulators for education, and arranges the supply of such simulation programs. Aware of this, in 2008 Gas Natural Fenosa, a Spanish gas and electric utility that owns and operate nuclear power plants and promotes university education in the nuclear technology field, provided the Department of Nuclear Engineering of Universidad Politécnica de Madrid with the Interactive Graphic Simulator (IGS) of “José Cabrera” (Zorita) nuclear power plant, an industrial facility whose commercial operation ceased definitively in April 2006. It is a state-of-the-art full-scope real-time simulator that was used for training and qualification of the operators of the plant control room, as well as to understand and analyses the plant dynamics, and to develop, qualify and validate its emergency operating procedures.

An ICT based Project Management Learning Framework

The objective of this paper is to present a framework that can facilitate the university level learning process in the Project Management of different students who are enrolled in different universities in different locations and attending their own Project Management courses, but running a virtual experience in executing and managing projects. The framework includes both information systems and methodological procedures that are integrated in the information system, making it possible to assess learning performance.

Assessment of learning outcomes in computing studies

The assessment of learning outcomes is a key concept in the European Credit Transfer and Accumulation System (ECTS) since credits are awarded when the assessment shows the competences which were aimed at have been developed at an appropriate level. This paper describes a study which was first part of the project of the Bologna Experts Team-Spain and then developed as an independent study. It was carried out with the overall goal to gain experience in the assessment of learning outcomes. More specifically it aimed at 1) designing procedures for the assessment of learning outcomes related to these compulsory generic competences; 2) testing some basic psychometric features that an assessment device with some consequences for the subjects being evaluated needs to prove; 3) testing different procedures of standard setting, and 4) using assessment results as orienting feedback to students and their tutors. The process of development of tests to carry out the assessment of learning outcomes related to these competences, as well as some basic features regarding their reliability and validity is described and first results on the comparison of results achieved at two academic levels, will also be described at a later stage.

Cooperative Learning Model based on Multi-Agent Architecture for Embedded Intelligent Systems

Cooperative systems are suitable for many types of applications and nowadays these system are vastly used to improve a previously defined system or to coordinate multiple devices working together. This paper provides an alternative to improve the reliability of a previous intelligent identification system. The proposed approach implements a cooperative model based on multi-agent architecture. This new system is composed of several radar-based systems which identify a detected object and transmit its own partial result by implementing several agents and by using a wireless network to transfer data. The proposed topology is a centralized architecture where the coordinator device is in charge of providing the final identification result depending on the group behavior. In order to find the final outcome, three different mechanisms are introduced. The simplest one is based on majority voting whereas the others use two different weighting voting procedures, both providing the system with learning capabilities. Using an appropriate network configuration, the success rate can be improved from the initial 80% up to more than 90%.