Softening-up mannan-rich cell walls

The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the predominant hemicellulosic CW polymers in many genera of green algae. The endosperm CWs of dry seeds often contain mannan polymers, sometimes in the form of galactomannans (Gal-mannans). The endo-beta-mannanases (MANs) that catalyse the random hydrolysis of the beta-linkage in the mannan backbone are one of the main hydrolytic enzymes involved in the loosening and remodelling of CWs. In germinating seeds, the softening of the endosperm seed CWs facilitates the emergence of the elongating radicle. Hydrolysis and mobilization of endosperm Gal-mannans by MANs also provides a source of nutrients for early seedling growth, since Gal-mannan, besides its structural role, serves as a storage polysaccharide. Therefore, the role of mannans and of their hydrolytic enzymes is decisive in the life cycle of seeds. This review updates and discusses the significance of mannans and MANs in seeds and explores the increasing biotechnological potential of MAN enzymes.

Drifting plasma collection by a positive biased tether wire in LEO-like plasma conditions: current measurement and plasma diagnostic

BETs is a three-year project financed by the Space Program of the European Commission, aimed at developing an efficient deorbit system that could be carried on board any future satellite launched into Low Earth Orbit (LEO). The operational system involves a conductive tape-tether left bare to establish anodic contact with the ambient plasma as a giant Langmuir probe. As a part of this project, we are carrying out both numerical and experimental approaches to estimate the collected current by the positive part of the tether. This paper deals with experimental measurements performed in the IONospheric Atmosphere Simulator (JONAS) plasma chamber of the Onera-Space Environment Department. The JONAS facility is a 9- m3 vacuum chamber equipped with a plasma source providing drifting plasma simulating LEO conditions in terms of density and temperature. A thin metallic cylinder, simulating the tether, is set inside the chamber and polarized up to 1000 V. The Earth's magnetic field is neutralized inside the chamber. In a first time, tether collected current versus tether polarization is measured for different plasma source energies and densities. In complement, several types of Langmuir probes are used at the same location to allow the extraction of both ion densities and electron parameters by computer modeling (classical Langmuir probe characteristics are not accurate enough in the present situation). These two measurements permit estimation of the discrepancies between the theoretical collection laws, orbital motion limited law in particular, and the experimental data in LEO-like conditions without magnetic fields. In a second time, the spatial variations and the time evolutions of the plasma properties around the tether are investigated. Spherical and emissive Langmuir probes are also used for a more extensive characterization of the plasma in space and time dependent analysis. Results show the ion depletion because of the wake effect and the accumulation of- ions upstream of the tether. In some regimes (at large positive potential), oscillations are observed on the tether collected current and on Langmuir probe collected current in specific sites.

Modelización de las fallas activas para la estimación de la Peligrosidad Sísmica. Aplicación Metodológica en Haití

The development of this Master's Thesis is aimed at modeling active for estimating seismic hazard in Haití failures. It has been used zoned probabilistic method, both classical and hybrid, considering the incorporation of active faults as independent units in the calculation of seismic hazard. In this case, the rate of seismic moment is divided between the failures and the area seismogenetic same region. Failures included in this study are the Septentrional, Matheux and Enriquillo fault. We compared the results obtained by both methods to determine the importance of considering the faults in the calculation. In the first instance, updating the seismic catalog, homogenization, completeness analysis and purification was necessary to obtain a catalog ready to proceed to the estimation of the hazard. With the seismogenic zoning defined in previous studies and the updated seismic catalog, they are obtained relations Gutenberg-Richter recurrence of seismicity, superficial and deep in each area. Selected attenuation models were those used in (Benito et al., 2011), as the tectonic area of study is very similar to that of Central America. Its implementation has been through the development of a logical in which each branch is multiplied by an index based on the relevance of each combination of models. Results are presented as seismic hazard maps for return periods of 475, 975 and 2475 years, and spectral acceleration (SA) in structural periods: 0.1 - 0.2 - 0.5 - 1.0 and 2.0 seconds, and the difference accelerations between maps obtained by the classical method and the hybrid method. Maps realize the importance of including faults as separate items in the calculation of the hazard. The morphology of the zoned maps presented higher values in the area where the superficial and deep zone overlap. In the results it can determine that the minimum values in the zoned approach they outweigh the hybrid method, especially in areas where there are no faults. Higher values correspond to those obtained in fault zones by the hybrid method understanding that the contribution of the faults in this method is very important with high values. The maximum value of PGA obtained is close to Septentrional in 963gal, near to 460 gal in Matheux, and the Enriquillo fault line value reaches 760gal PGA in the Eastern segment and Western 730gal in the segment. This compares with that obtained in the zoned approach in this area where the value of PGA obtained was 240gal. These values are compared with those obtained by Frankel et al., (2011) with those have much similarity in values and morphology, in contrast to those presented by Benito et al., (2012) and the Standard Seismic Dominican Republic