Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya) and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp) was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers. AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were the most similar, in spite of their morphological diversity.

Investigations on the distribution of air transport traffic and CO2 emissions within the European Union

This study analyses the structure of air traffic and its distribution among the different countries in the European Union, as well as traffic with an origin or destination in non-EU countries. Data sources are Eurostat statistics and actual flight information from EUROCONTROL. Relevant variables such as the number of flights, passengers or cargo tonnes and production indicators (RPKs) are used together with fuel consumption and CO2 emissions data. The segmentation of air traffic in terms of distance permits an assessment of air transport competition with surface transport modes. The results show a clear concentration of traffic in the five larger countries (France, Germany, Italy, Spain and UK), in terms of RPKs. In terms of distance the segment between 500 and 1000 km in the EU, has more flights, passengers, RTKs and CO2 emissions than larger distances. On the environmental side, the distribution of CO2 emissions within the EU Member States is presented, together with fuel efficiency parameters. In general, a direct relationship between RPKs and CO2 emissions is observed for all countries and all distance bands. Consideration is given to the uptake of alternative fuels. Segmenting CO2 emissions per distance band and aircraft type reveals which flights contribute the most the overall EU CO2 emissions. Finally, projections for future CO2 emissions are estimated, according to three different air traffic growth and biofuel introduction scenarios.

Criterios para el dimensionamiento de redes de riego robustas frente a cambios en la alternativa de cultivos

En los años 50 y 60 del siglo XX se produjo en Europa un cambio en la concepción de los sistemas de riego, en los cuales se pasó del regadío por superficie al presurizado y con ello del riego por turnos al riego a la demanda. Las ventajas de este nuevo sistema de riego dieron lugar a una gran expansión de las redes colectivas de riego a presión, especialmente en los países del arco mediterráneo y del Sur de Europa (Francia, España, Italia, Portugal y Grecia). Desde entonces el riego presurizado a la demanda ha tenido una evolución permanente, en la que han mejorado los equipos y las técnicas de aplicación del riego, siempre con una clara orientación hacía el incremento de la productividad. Esta evolución unida a los vaivenes de los mercados, al abaratamiento de los transportes y la globalización, y a las subvenciones agrícolas, ha propiciado que las alternativas de cultivos previstas hayan sufrido cambios. El cambio de la alternativa de cultivos hacia otros más exigentes desde el punto de vista de las necesidades hídricas tiene como consecuencia el aumento de los consumos, circunstancia para cual debe estar capacitada la red. Otros fenómenos como el cambio climático, de amplio interés a día de hoy, presentan algunos escenarios en lo que se prevé un incremento de las temperaturas que unido a una reducción en las precipitaciones, supondría también que se elevarían las necesidades de riego de los cultivos. Todas estas circunstancias deben ser contempladas en la redacción de los proyectos de transformación de nuevas zonas regables y en los de modernización de las existentes. Las redes de riego deben estar dimensionadas para poder atender dichos incrementos de consumo. La fortaleza de la red para atender variaciones en las demandas de agua se convierte por tanto en una premisa del proyecto. Dicha fortaleza se puede conseguir de formas diferentes y a costos distintos, puesto que en el proceso de dimensionamiento estadístico de los caudales circulantes en punta de campaña intervienen muchas variables y parámetros de riego. En la presente tesis doctoral se analiza en detalle el estado del arte en la materia, se efectúa un análisis de la función específica y la influencia en el cálculo de cada uno de los parámetros, se establece un procedimiento de actuación que optimice el dimensionamiento de la red colectiva para que ésta disponga de la robustez necesaria para hacer frente a incrementos potenciales de consumo de agua sobre la previsiones de base del proyecto y se establecen los criterios, rangos y combinaciones de parámetros que permiten dotar a la red de la fortaleza necesaria de la manera más eficiente posible. During the 1950s and 1960s the design of irrigation systems in Europe underwent change. Traditional surface irrigation was replaced by pressure irrigation, with the delivery method shifting from a scheduled type to an on-demand one. The advantages obtained with this new system led to a significant development of on-demand pressurised irrigation districts, especially in the Southern countries (France, Greece, Italy, Portugal and Spain). On-demand pressurised irrigation has since evolved in a continuous manner in which, with the purpose of gaining productivity, on-farm equipment and automation instruments have seen improvements. Such developments, along with market fluctuations, reductions in transport costs, globalisation and the influence of agricultural subsidies, have resulted in changes in the crop pattern. The farming of new crops may require more water and the consumption may become greater. In addition to this, other phenomena, such as the frequently-debated effects of climate change, reveal scenarios in which an increase in temperatures and the accompanying reductions in rainfall are expected. One consequence of this would be an increase in irrigation requirements and subsequent impact on irrigation networks. All such circumstances should be taken into account in both the design of new irrigation districts and the upgrading of the existing ones. Irrigation networks should be of a size that allows them to meet increases in consumption. The robustness of the network, defined as its capacity to absorb increments in water requirements, becomes a key point to be taken into account in the design phase. Given that there are several parameters that influence the calculus of design flows, such robustness may be obtained in different ways and at varying costs. In the context of this, the thesis reviews the state of the art, analyses the role and influence of each parameter, establishes a procedure for checking the design and robustness of on-demand irrigation networks, and sets design criteria for selecting the most effective range and combination of parameters that provide the network with such robustness.

Consideraciones y recomendaciones para el diseño de estructuras bajo amenaza de tsunamis

La visión del riesgo es muy importante a la hora de diseñar y construir estructuras. Sobre todo en presencia de catástrofes naturales. Esta tesis presenta las consideraciones y recomendaciones a tener en cuenta para proyectar estructuras en zonas con riesgo de tsunami, tras haber estudiado todos los riesgos y soluciones posibles mediante tres técnicas diferentes. Esto se ha obtenido aplicando el método LOGRO (Líder de Organización de Gestión de Riesgos y Oportunidades). Se puede pensar que la única solución posible ante esta catástrofe es no construir en zonas vulnerables, pero no es posible. Hay que tener en cuenta que no todos los tsunamis son de la misma magnitud e intensidad y este trabajo contempla todos ellos con distintos niveles de actuación para cada situación. Por otro lado, existen lugares costeros muy urbanizados en los que sería imposible fomentar la emigración, y zonas turísticas donde lo que prima es la cercanía a la costa. Las resultados obtenidos de esta investigación tienen como objetivo reducir el riesgo vinculado al tsunami, minimizando tanto probabilidades de ocurrencia como consecuencias. The hazard view is very important when designing and building structures, mainly, under natural disasters. This thesis presents the considerations and recommendations to take into account to project structures in tsunami prone areas after having studied all the hazards and possible solutions through three different techniques. This has been obtained using LOGRO´s method. It is easy to think that the only possible solution to this disaster is not to build in vulnerable areas, but it is not like this. It has to be taken into account that not all tsunamis are of the same magnitude neither of the same intensity and this work considers all of them with different performance levels for each situation. On the other hand, there are very developed coastal places where it would be impossible to encourage emigration, and touristic areas where the closeness to the coast is what comes first. The aim of the results obtained in this research is to reduce the tsunami hazard minimising not only probabilities but also consequences.