4 resultados para Family Members
em Universidad Politécnica de Madrid
Resumo:
Phytohormones regulate a wide array of developmental processes throughout the life cycle of plants. Over recent years, mounting evidence led to the widely accepted concept that plant hormone action is not the read-out of linear pathways, but determined by the extensive combinatorial activity of the signaling molecules and the integration of their signaling pathways, both in terms of regulating growth and development and in adapting to external stimuli. Recent work is beginning to shed light on the crosstalk of both nominally synergistically and antagonistically acting plant hormones such as, for example, auxins with oxylipins. Here, we report that oxylipins directly contribute to the regulation of the expression of two Arabidopsis YUCCA (YUC) genes, YUC8 and YUC9. Similar to previously characterized YUC family members, we identify both YUC8 and YUC9 as involved in local auxin biosynthesis, as demonstrated by the altered auxin contents and auxin-dependent phenotypes displayed by loss-of function mutants and transgenic overexpressing lines. Gene expression data obtained by qPCR analysis and microscopic examination of promoter-reporter lines reveal an oxylipin-mediated regulation of YUC9 expression that is dependent on the COI1 signal transduction pathway. The microscopic data indicate a functional overlap of the two analyzed auxin biosynthesis genes, but also point out specific functions for YUC8 and YUC9, which are in part related to different spatio-temporal expression pattern. In support of these findings, the analyzed yuc knockout mutants had lower free auxin contents and displayed a reduced response to oxylipins. This work provides evidence of a molecular mechanism that links oxylipin signaling with auxin homeostasis.
Resumo:
En la coyuntura actual, en la que existe por un lado, exceso en la oferta de vivienda (de alto precio o de segunda residencia), y aparece por otro demanda de vivienda (de bajo precio y/o social), el mercado inmobiliario se encuentra paradójicamente bloqueado. Así, surge esta investigación como fruto de este momento histórico, en el cual se somete a debate económico el producto vivienda, no solo como consecuencia de la profunda crisis económica, sino también para la correcta gestión de los recursos desde el punto de vista de lo eficiente y sostenible. Se parte de la hipótesis de que es necesario determinar un estimador de costes de construcción de vivienda autopromovida como una de las soluciones a la habitación en el medio rural de Extremadura, para lo cual se ha tomado como modelo de análisis concretamente la Vivienda Autopromovida subvencionada por la Junta de Extremadura en el marco de la provincia de Cáceres. Con esta investigación se pretende establecer una herramienta matemática precisa que permita determinar la inversión a los promotores, el posible margen de beneficios a los contratistas y el valor real de la garantía en el préstamo a las entidades financieras. Pero el objetivo de mayor proyección social de esta investigación consiste en facilitar una herramienta sencilla a la Junta de Extremadura para que pueda establecer las ayudas de una manera proporcional. De este modo se ayuda a optimizar los recursos, lo cual en época de crisis resulta aun más acuciante, ya que conociendo previamente y con bastante exactitud el importe de las obras se pueden dirigir las ayudas de forma proporcional a las necesidades reales de la ejecución. De hecho, ciertas características difíciles de cuantificar para determinar las ayudas en materia de vivienda, como la influencia del número de miembros familiares o la atención a la discapacidad, se verían contempladas de forma indirecta en el coste estimado con el método aquí propuesto, ya que suponen siempre un aumento de las superficies construidas y útiles, de los huecos de fachadas o del tamaño de locales húmedos y por tanto se contemplan en la ecuación del modelo determinado. Por último, contar con un estimador de costes potencia la forma de asentamiento de la construcción mediante autopromocion de viviendas ya que ayuda a la toma de decisiones al particular, subvencionado o no. En efecto, la herramienta es valida en cierta medida para cualquier autopromocion, constituye un sistema de construcción con las menores posibilidades especulativas y lo más sostenible, es abundante en toda Extremadura, y consigue que el sector de la construcción sea un sistema más eficiente al optimizar su proceso económico de producción. SUMMARY Under the present circumstances, in which there is, on one hand, an excess in the supply of housing (high-price or second-home), and on the other hand a demand for housing (low cost and/or social), paradoxically the property market is at a standstill. This research has come about as a result of this moment in time, in which the product: housing, is undergoing economic debate, not only on account of this serious economic crisis, but for the proper management of resources from the point of view of efficiency and sustainability. A building-costs estimator for owner-developed housing is deemed necessary as one of the solutions for the rural environment that is Extremadura. To this end, it is the Owner-Developed House which has been taken as analysis model. It is subsidized by the Extremadura Regional Government in Caceres Province. This research establishes an accurate mathematical tool to work out the developers’ investment, the builder’s potential profit margin and the reality of the loan for the Financial Institution. But the result of most social relevance in this research is to provide the Extremadura Regional Government with a simple tool, so that it can draw up the Subventions proportionally. Thus, the resources are optimized, an even more vital matter in times of economic slump, due to the fact that if the cost of the building works is known with some accuracy beforehand, the subventions can be allocated in a way that is proportional to the real needs of execution. In fact certain elements related to housing subventions which are hard to quantify, such as the influence of number of family members or disability support, would be covered indirectly in cost estimate with the proposed method, since they inevitably involve an increase in built area, exterior wall openings and the size of plumbed rooms. As such they are covered in the determined model equation. Lastly, the availability of a cost-estimator reinforces the ownerdeveloped building model, since it assists decision-making by the individual, whether subsidized or not. This is because the tool is valid to some extent in any owner-development, and this building scheme, which is common in Extremadura, is the most sustainable, and the least liable to speculation. It makes the building sector more efficient by optimizing the economic production process.
Resumo:
Senescence-associated proteolysis in plants is a crucial process to relocalize nutrients from leaves to growing or storage tissues. The massive net degradation of proteins involves broad metabolic networks, different subcellular compartments, and several types of proteases and regulators. C1A cysteine proteases, grouped as cathepsin L-, B-, H-, and F-like according to their gene structures and phylogenetic relationships, are the most abundant enzymes responsible for the proteolytic activity during leaf senescence. Besides, cystatins as specific modulators of C1A peptidase activities exert a complex regulatory role in this physiological process. This overview article covers the most recent information on C1A proteases in leaf senescence in different plant species. Particularly, it is focussed on barley, as the unique species where the whole gene family members of C1A cysteine proteases and cystatins have been analysed.
Resumo:
Leaf senescence is a recycling process characterized by a massive degradation of macromolecules to relocalize nutrients from leaves to growing or storage tissues. Our aim is to identify and analyze the C1A Cysteine ‐Protease (CysProt) family members from barley (35 cathepsin L‐,3B‐,1Hand3F‐like) involved in leaf senescence, to study their modulation by their specific inhibitors (cystatins) and to determine their roles mediated by abiotic (darkness and N starvation) and biotic (pathogens and pest) stresses.