3 resultados para Dorsal-ventral pattern formation
em Universidad Politécnica de Madrid
Resumo:
Esta tesis se centra en la generación de ondas superficiales subarmónicas en fluidos sometidos a vibración forzada en el régimen gravitatorio capilar con líquidos de baja viscosidad. Tres problemas diferentes han sido estudiados: un contenedor rectangular con vibración horizontal, la misma geometría pero con una combinación de vibración vertical y horizontal y un obstáculo completamente sumergido vibrado verticalmente en un contenedor grande. Se deriva una ecuación de amplitud desde primeros principios para describir las ondas subarmónicas con forzamiento parámetrico inducido por la vibración. La ecuación es bidimensional mientras que el problema original es tridimensional y admite un forzamiento espacial no uniforme. Usando esta ecuación los tres sistemas han sido analizados, centrándose en calcular la amplitud crítica, la orientación de los patrones y el carácter temporal de los patrones espaciotemporales, que pueden ser estrictamente subarmónicos o cuasiperiodicos con una frecuencia de modulación temporal. La dependencia con los parámetros adimensionales también se considera. La teoría será comparada con los experimentos disponibles en la literatura. Abstract This thesis focus on the generation of subharmonic surface waves on fluids subject to forced vibration in the gravity-capillary regime with liquids of small viscosity. Three different problems have been considered: a rectangular container under horizontal vibration; the same geometry but under a combination of horizontal and vertical vibration; and a fully submerged vertically vibrated obstacle in a large container. An amplitude equation is derived from first principles that fairly precisely describes the subharmonic surfaces waves parametrically driven by vibration. That equation is two dimensional while the underlying problem is three-dimensional and permits spatially nonuniform forcing. Using this equation, the three systems have been analyzed, focusing on the calculation of the threshold amplitude, the pattern orientation, and the temporal character of the spatio-temporal patterns, which can be either strictly subharmonic or quasi-periodic, showing an additional modulation frequency. Dependence on the non-dimensional parameters is also considered. The theory is compared with the experiments available in the literature.
Resumo:
Macroscopic brain networks have been widely described with the manifold of metrics available using graph theory. However, most analyses do not incorporate information about the physical position of network nodes. Here, we provide a multimodal macroscopic network characterization while considering the physical positions of nodes. To do so, we examined anatomical and functional macroscopic brain networks in a sample of twenty healthy subjects. Anatomical networks are obtained with a graph based tractography algorithm from diffusion-weighted magnetic resonance images (DW-MRI). Anatomical con- nections identified via DW-MRI provided probabilistic constraints for determining the connectedness of 90 dif- ferent brain areas. Functional networks are derived from temporal linear correlations between blood-oxygenation level-dependent signals derived from the same brain areas. Rentian Scaling analysis, a technique adapted from very- large-scale integration circuits analyses, shows that func- tional networks are more random and less optimized than the anatomical networks. We also provide a new metric that allows quantifying the global connectivity arrange- ments for both structural and functional networks. While the functional networks show a higher contribution of inter-hemispheric connections, the anatomical networks highest connections are identified in a dorsal?ventral arrangement. These results indicate that anatomical and functional networks present different connectivity organi- zations that can only be identified when the physical locations of the nodes are included in the analysis.
Resumo:
To determine the contribution of polar auxin transport (PAT) to auxin accumulation and to adventitious root (AR) formation in the stem base of Petunia hybrida shoot tip cuttings, the level of indole-3-acetic acid (IAA) was monitored in non-treated cuttings and cuttings treated with the auxin transport blocker naphthylphthalamic acid (NPA) and was complemented with precise anatomical studies. The temporal course of carbohydrates, amino acids and activities of controlling enzymes was also investigated. Analysis of initial spatial IAA distribution in the cuttings revealed that approximately 40 and 10% of the total IAA pool was present in the leaves and the stem base as rooting zone, respectively. A negative correlation existed between leaf size and IAA concentration. After excision of cuttings, IAA showed an early increase in the stem base with two peaks at 2 and 24h post excision and, thereafter, a decline to low levels. This was mirrored by the expression pattern of the auxin-responsive GH3 gene. NPA treatment completely suppressed the 24-h peak of IAA and severely inhibited root formation. It also reduced activities of cell wall and vacuolar invertases in the early phase of AR formation and inhibited the rise of activities of glucose-6-phosphate dehydrogenase and phosphofructokinase during later stages. We propose a model in which spontaneous AR formation in Petunia cuttings is dependent on PAT and on the resulting 24-h peak of IAA in the rooting zone, where it induces early cellular events and also stimulates sink establishment. Subsequent root development stimulates glycolysis and the pentosephosphate pathway