Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors

The plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell-wall disruption leads to accumulation of jasmonates (JAs), which are key signaling molecules for activation of plant inducible defense responses. However, whether JAs in return modulate the cell-wall composition to reinforce this defensive barrier remains unknown. The enzyme 13-allene oxide synthase (13-AOS) catalyzes the first committed step towards biosynthesis of JAs. In potato (Solanum tuberosum), there are two putative St13-AOS genes, which we show here to be differentially induced upon wounding. We also determine that both genes complement an Arabidopsis aos null mutant, indicating that they encode functional 13-AOS enzymes. Indeed, transgenic potato plants lacking both St13-AOS genes (CoAOS1/2 lines) exhibited a significant reduction of JAs, a concomitant decrease in wound-responsive gene activation, and an increased severity of soft rot disease symptoms caused by Dickeya dadantii. Intriguingly, a hypovirulent D. dadantii pel strain lacking the five major pectate lyases, which causes limited tissue maceration on wild-type plants, regained infectivity in CoAOS1/2 plants. In line with this, we found differences in pectin methyl esterase activity and cell-wall pectin composition between wild-type and CoAOS1/2 plants. Importantly, wild-type plants had pectins with a lower degree of methyl esterification, which are the substrates of the pectate lyases mutated in the pel strain. These results suggest that, during development of potato plants, JAs mediate modification of the pectin matrix to form a defensive barrier that is counteracted by pectinolytic virulence factors from D. dadantii.

STONE: a stream-based DDoS defense framework

An effective Distributed Denial of Service (DDoS) defense mechanism must guarantee legitimate users access to an Internet service masking the effects of possible attacks. That is, it must be able to detect threats and discard malicious packets in a online fashion. Given that emerging data streaming technology can enable such mitigation in an effective manner, in this paper we present STONE, a stream-based DDoS defense framework, which integrates anomaly-based DDoS detection and mitigation with scalable data streaming technology. With STONE, the traffic of potential targets is analyzed via continuous data streaming queries maintaining information used for both attack detection and mitigation. STONE provides minimal degradation of legitimate users traffic during DDoS attacks and it also faces effectively flash crowds. Our preliminary evaluation based on an implemented prototype and conducted with real legitimate and malicious traffic traces shows that STONE is able to provide fast detection and precise mitigation of DDoS attacks leveraging scalable data streaming technology.

Transformaciones urbanas en torno a las reales puertas de la Villa de Madrid (1656-1860)

La estructura urbana de Madrid comenzó a dibujarse con los primeros asentamientos fortificados del siglo IX. Sus posteriores ampliaciones estuvieron acotadas por los sucesivos recintos que delimitaron los contornos de una capital en constante expansión. De carácter inicialmente defensivo, luego fiscal y sanitario, estas estructuras estuvieron articuladas en torno a las puertas de acceso a la Villa, estableciendo un sistema general de cerramiento y comunicación que permitiera el control de personas y mercancías en su tránsito hacia el interior de la ciudad. La modestia inicial con que las puertas y tapias del recinto edificado en tiempos de Felipe IV desempeñaron sus funciones de espaldas al exterior de la Villa fue remplazada por un creciente protagonismo, de carácter simbólico y ornamental, que trascendió a su propia arquitectura para inspirar algunas de las importantes transformaciones urbanas operadas en su entorno. Relacionada principalmente con el ideal ilustrado de embellecimiento de la ciudad, la renovación de sus puertas principales se completaba con la reedificación y regularización de sus cercas, y la conformación de nuevos paseos en las afueras de la capital, cuyo trazado vertebrará en buena medida la ocupación de la periferia y la consiguiente definición de la trama urbana del Madrid de hoy. El presente trabajo de investigación indaga sobre la significación urbana de las Reales Puertas de la Villa de Madrid, a partir de la revisión de su establecimiento en los sucesivos recintos de la capital, con especial atención a las transformaciones urbanas operadas en ella desde la definición de su último límite hasta la proyección futura del Madrid ampliado según el anteproyecto de Carlos Mª de Castro. La observación conjunta de las componentes arquitectónica y urbana de las puertas de la Villa de Madrid se ofrece a partir de un relato cronológico de los hechos, fundamentado en su justificación documental y la secuencia visual registrada en la cartografía histórica de la ciudad. La incorporación de aportaciones gráficas de nueva elaboración, de carácter y alcance diversos, proporciona una superposición espacio-temporal que posibilita la lectura comparada de las arquitecturas de las Reales Puertas de la Villa de Madrid y de las transformaciones urbanas operadas a partir de ellas, determinantes en gran medida la configuración de la ciudad actual. ABSTRACT Madrid’s current urban structure has its roots in the first fortified settlements of the IX century. Its subsequent expansions due to the capital’s constant growth were limited by successive enclosures, built originally as a defense mechanism, but later used for fiscal and sanitary purposes as well. The construction of these structures pivoted around the gates that gave access to the city, establishing an enclosure that allowed control of both people and goods on their way into the city. The gates and walls originally built by Felipe IV performed their purpose with a modesty that was later replaced by an increasing symbolic and ornamental prominence, eventually surpassing their own architecture to inspire profound urban changes around them. With the purpose of embellishing the city, the main gates were renovated, the walls were rebuilt and standardized, and new avenues were laid out outside the city. These changes dictated in large part the settling on the suburbs and the resulting configuration of Madrid’s urban scene as we know it today. This research explores the urban significance of the Royal Gates of Madrid through the study of the enclosures that marked the limits of the city. Special attention is given to the urban changes since the last enclosure was established through to Carlos Mª de Castro’s draft for Madrid’s future projection. The architectonic and urban facets of Madrid’s gates are examined simultaneously in a series of chronological events, based on relevant documentation and the graphical record found in Madrid’s historic cartography. This thesis includes new graphic contributions, which allow the comparison of the architecture of the Royal Gates of Madrid as they evolved in time and space. These documents are essential in order to understand the urban transformations that took place based on the Gates, having largely determined the city’s current configuration.