Molecular tools to improve chestnut management: El Bierzo as a case study

The European chestnut (Castanea sativa Mill.) is a multipurpose species that has been widely cultivated around the Mediterranean basin since ancient times. New varieties were brought to the Iberian Peninsula during the Roman Empire, which coexist since then with native populations that survived the last glaciation. The relevance of chestnut cultivation has being steadily growing since the Middle Ages, until the rural decline of the past century put a stop to this trend. Forest fires and diseases were also major factors. Chestnut cultivation is gaining momentum again due to its economic (wood, fruits) and ecologic relevance, and represents currently an important asset in many rural areas of Europe. In this Thesis we apply different molecular tools to help improve current management strategies. For this study we have chosen El Bierzo (Castile and Leon, NW Spain), which has a centenary tradition of chestnut cultivation and management, and also presents several unique features from a genetic perspective (next paragraph). Moreover, its nuts are widely appreciated in Spain and abroad for their organoleptic properties. We have focused our experimental work on two major problems faced by breeders and the industry: the lack of a fine-grained genetic characterization and the need for new strategies to control blight disease. To characterize with sufficient detail the genetic diversity and structure of El Bierzo orchards, we analyzed DNA from 169 trees grafted for nut production covering the entire region. We also analyzed 62 nuts from all traditional varieties. El Bierzo constitutes an outstanding scenario to study chestnut genetics and the influence of human management because: (i) it is located at one extreme of the distribution area; (ii) it is a major glacial refuge for the native species; (iii) it has a long tradition of human management (since Roman times, at least); and (iv) its geographical setting ensures an unusual degree of genetic isolation. Thirteen microsatellite markers provided enough informativeness and discrimination power to genotype at the individual level. Together with an unexpected level of genetic variability, we found evidence of genetic structure, with three major gene pools giving rise to the current population. High levels of genetic differentiation between groups supported this organization. Interestingly, genetic structure does not match with spatial boundaries, suggesting that the exchange of material and cultivation practices have strongly influenced natural gene flow. The microsatellite markers selected for this study were also used to classify a set of 62 samples belonging to all traditional varieties. We identified several cases of synonymies and homonymies, evidencing the need to substitute traditional classification systems with new tools for genetic profiling. Management and conservation strategies should also benefit from these tools. The avenue of high-throughput sequencing technologies, combined with the development of bioinformatics tools, have paved the way to study transcriptomes without the need for a reference genome. We took advantage of RNA sequencing and de novo assembly tools to determine the transcriptional landscape of chestnut in response to blight disease. In addition, we have selected a set of candidate genes with high potential for developing resistant varieties via genetic engineering. Our results evidenced a deep transcriptional reprogramming upon fungal infection. The plant hormones ET and JA appear to orchestrate the defensive response. Interestingly, our results also suggest a role for auxins in modulating such response. Many transcription factors were identified in this work that interact with promoters of genes involved in disease resistance. Among these genes, we have conducted a functional characterization of a two major thaumatin-like proteins (TLP) that belongs to the PR5 family. Two genes encoding chestnut cotyledon TLPs have been previously characterized, termed CsTL1 and CsTL2. We substantiate here their protective role against blight disease for the first time, including in silico, in vitro and in vivo evidence. The synergy between TLPs and other antifungal proteins, particularly endo-p-1,3-glucanases, bolsters their interest for future control strategies based on biotechnological approaches.

Polyolefin fibre-reinforced concrete : from material behaviour to numerical and design considerations

La presente Tesis proporciona una gran cantidad de información con respecto al uso de un nuevo y avanzado material polimérico (con base de poliolefina) especialmente adecuada para ser usada en forma de fibras como adición en el hormigón. Se han empleado fibras de aproximadamente 1 mm de diámetro, longitudes entre 48 y 60 mm y una superficie corrugada. Las prometedoras propiedades de este material (baja densidad, bajo coste, buen comportamiento resistente y gran estabilidad química) justifican el interés en desarrollar el esfuerzo de investigación requerido para demostrar las ventajas de su uso en aplicaciones prácticas. La mayor parte de la investigación se ha realizado usando hormigón autocompactante como matriz, ya que este material es óptimo para el relleno de los encofrados del hormigón, aunque también se ha empleado hormigón normal vibrado con el fin de comparar algunas propiedades. Además, el importante desarrollo del hormigón reforzado con fibras en los últimos años, tanto en investigación como en aplicaciones prácticas, también es muestra del gran interés que los resultados y consideraciones de diseño que esta Tesis pueden tener. El material compuesto resultante, Hormigón Reforzado con Fibras de Poliolefina (HRFP o PFRC por sus siglas inglesas) ha sido exhaustivamente ensayado y estudiado en muchos aspectos. Los resultados permiten establecer cómo conseguidos los objetivos buscados: -Se han cuantificado las propiedades mecánicas del PFRC con el fin de demostrar su buen comportamiento en la fase fisurada de elementos estructurales sometidos a tensiones de tracción. -Contrastar los resultados obtenidos con las bases propuestas en la normativa existente y evaluar las posibilidades para el uso del PFRC con fin estructural para sustituir el armado tradicional con barras de acero corrugado para determinadas aplicaciones. -Se han desarrollado herramientas de cálculo con el fin de evaluar la capacidad del PFRC para sustituir al hormigón armado con las barras habituales de acero. -En base a la gran cantidad de ensayos experimentales y a alguna aplicación real en la construcción, se han podido establecer recomendaciones y consejos de diseño para que elementos de este material puedan ser proyectados y construidos con total fiabilidad. Se presentan, además, resultados prometedores en una nueva línea de trabajo en el campo del hormigón reforzado con fibras combinando dos tipologías de fibras. Se combinaron fibras de poliolefina con fibras de acero como refuerzo del mismo hormigón autocompactante detectándose sinergias que podrían ser la base del uso futuro de esta tecnología de hormigón. This thesis provides a significant amount of information on the use of a new advanced polymer (polyolefin-based) especially suitable in the form of fibres to be added to concrete. At the time of writing, there is a noteworthy lack of research and knowledge about use as a randomly distributed element to reinforce concrete. Fibres with an approximate 1 mm diameter, length of 48-60 mm, an embossed surface and improved mechanical properties are employed. The promising properties of the polyolefin material (low density, inexpensive, and with good strength behaviour and high chemical stability) justify the research effort involved and demonstrate the advantages for practical purposes. While most of the research has used self-compacting concrete, given that this type of matrix material is optimum in filling the concrete formwork, for comparison purposes standard vibration compacted mixes have also been used. In addition, the interest in fibre-reinforced concrete technology, in both research and application, support the significant interest in the results and considerations provided by the thesis. The resulting composite material, polyolefin fibre reinforced concrete (PFRC) has been extensively tested and studied. The results have allowed the following objectives to be met: -Assessment of the mechanical properties of PFRC in order to demonstrate the good performance in the post-cracking strength for structural elements subjected to tensile stresses. -- Assessment of the results in contrast with the existing structural codes, regulations and test methods. The evaluation of the potential of PFRC to meet the requirements and replace traditional steel-bar reinforcement applications. -Development of numerical tools designed to evaluate the capability of PFRC to substitute, either partially or totally, standard steel reinforcing bars either alone or in conjunction with steel fibres. -Provision, based on the large amount of experimental work and real applications, of a series of guidelines and recommendations for the practical and reliable design and use of PFRC. Furthermore, the thesis also reports promising results about an innovative line in the field of fibre-reinforced concrete: the design of a fibre cocktail to reinforce the concrete by using two types of fibres simultaneously. Polyolefin fibres were combined with steel fibres in self-compacting concrete, identifying synergies that could serve as the base in the future use of fibre-reinforced concrete technology.

Influencia de un recubrimiento de vinilo en la tensión de fractura del vidrio en ensayos de anillos concéntricos con gran superficie

El comportamiento post-rotura de los vidrios laminados es uno de los temas que están siendo investigados para explicar la capacidad de carga remanente tras la rotura de la primera lámina. En investigaciones previas se ha observado que en el caso de impacto humano en vidrios recocidos se llega a una capacidad hasta 3 veces superior, sin explicación clara del comportamiento estructural del conjunto. Para realizar un acercamiento a la resistencia a la rotura del vidrio laminado se ha planificado una campaña de ensayos de rotura con anillos concéntricos de grandes superficies en vidrio recocido, termoendurecido y templado, con dos series adicionales de vidrio recocido y termoendurecido con una capa de butiral adherida justo después del proceso de fabricación. Para realizar la comparación de las distribuciones de Weibull de las distintas tensiones de rotura se utiliza un proceso iterativo basado en la distribución real de tensiones obtenida con un modelo de elementos finitos ajustado con datos experimentales. Las comparaciones finales muestran un aumento apreciable de la resistencia (45%) en el caso de vidrios recocidos, y menor en el de los termoendurecidos (25%).The post-fracture behavior of the laminated glasses is one of the research topics that are being studied to explain the load capacity after the break of the first sheet. Previous experimental work have shown, that in case of human impact in annealed glasses, the capacity of bearing load it can be up to 3 times higher without clear explanation of the structural behavior of the plate. To make an approximation to the post-fracture resistance, a experimental program to test annealed, heat-tempered and toughened glass plates has been prepared. Two additional series of annealed and heattempered, with a layer of polyvinyl butyral adhered just after the manufacturing process, have also been incorporated. Coaxial Double Ring with large test surface areas Coaxial Double Ring with large test surface areas is the standard that has been followed. To make the comparison of Weibull's distributions of the different fracture stress, an iterative process based on the actual stress distribution obtained with a finite elements model updated with experimental results has been used. Final comparisons show a great stress improvement for the annealed glass plates (45 %), and a minor increment for the heat-tempered (25 %).

Contribute to the Study of Vertical Distribution of Conventional No-Till Seeders and Spatial Variability of Seed Depth Placement of Maize in Alentejo, Portugal

Entre os vários fatores que contribuem para a produção de uma cultura de milho, a distribuição vertical dos semeadores avaliada através da localização da semente em profundidade é um fator-chave, especialmente na técnica de sementeira direta. Simultaneamente, dada a complexidade dos ecossistemas naturais e agrícolas em sistemas de agricultura de conservação, a gestão diferenciada e localizada das parcelas assume um importante papel na análise e gestão da variabilidade das propriedades do solo e estabelecimento das culturas, nomeadamente utilizando informação geo referenciada e tecnologia expedita. Assim, o principal objetivo desta Tese foi a avaliação em culturas de milho da variabilidade espacial da localização de semente em profundidade e estabelecimento da cultura em sementeira direta usando sistemas convencionais de controlo de profundidade, tendo-se comparado com diferentes sistemas de mobilização e recorrendo a tecnologias de agricultura de precisão. Os ensaios decorreram na região Mediterrânea do Alentejo, em propriedades agrícolas no decorrer das campanhas de 2010, 2011, 2012 e 2015 em 6 diferentes campos experimentais. O trabalho experimental consistiu em ensaios com avaliações in loco do solo e cultura, consumo de combustível das operações e deteção remota. Os resultados obtidos indicam que não só o sistema de mobilização afetou a localização da semente em profundidade, como em sementeira direta a profundidade de sementeira foi afetada pelo teor de humidade do solo, resistência do solo à profundidade e velocidade da operação de sementeira. Adicionalmente observaram-se condições heterogéneas de emergência e estabelecimento da cultura afetadas por condições físicas de compactação do solo. Comparando os diferentes sistemas de mobilização, obteve-se uma significativa redução de combustível para a técnica de sementeira direta, apesar de se terem observado diferenças estatísticas significativas considerando diferentes calibrações de profundidade de sementeira Do trabalho realizado nesta Tese ressalva-se a importância que as tecnologias de agricultura de precisão podem ter no acompanhamento e avaliação de culturas em sementeira direta, bem como a necessidade de melhores procedimentos no controlo de profundidade dos semeadores pelo respetivos operadores ou ao invés, a adoção de semeadores com mecanismos ativos de controlo de profundidade. ABSTRACT Among the various factors that contribute towards producing a successful maize crop, seeders vertical distribution evaluated through seed depth placement is a key determinant, especially under a no-tillage technique. At the same time in conservation agriculture systems due to the complexity of natural and agricultural ecosystems site specific management became an important approach to understand and manage the variability of soil properties and crop establishment, especially when using geo spatial information and affording readily technology Thus, the main objective of this Thesis was to evaluate the spatial variability of seed depth placement and crop establishment in maize crops under no-tillage conditions compared to different tillage systems, using conventional seed depth control no till seeders and precision farming technologies. Trials were carried out in the Mediterranean region of Alentejo, in private farms along the sowing operations season over the years 2010, 2011, 2012 and 2015 in 6 different experimental fields. Experimental work covered field tests with in loco soil and crop evaluations, fuel operation evaluations and aerial sensing. The results obtained indicate that not only tillage system affected seed depth placement but under no till conditions seed depth was affected by soil moisture content, soil resistance to penetration and seeders forward speed. In addition uneven crop seedling and establishment depended on seed depth placement and could be affected by physical problems of compaction layers. Significant reduction in fuel consumption was observed for no till operations although significant differences observed according to different setting calibrations of seed depth control. According to the results, precision agriculture is an important tool to evaluate crops under no till conditions and seed depth mechanisms should be more accurate by the operators or is determinant the adoption of new active depth control technology to improve seeders performance.

Análisis teórico-experimental de pilares y vigas de hormigón armado reforzados con fibra de carbono

El presente trabajo se refiere al estudio teórico-experimental del comportamiento de pilares y vigas de hormigón armado reforzados con fibra de carbono o CFRP. El análisis se realiza considerando que los pilares se refuerzan mediante la técnica de adhesión de tejidos de fibra de carbono, generando un efecto de confinamiento. Las vigas se refuerzan mediante la incorporación de barras del mismo material, con refuerzos a cortante. El objetivo es poder comparar el estudio analítico de este tipo de refuerzos con resultados experimentales obtenidos con anterioridad a la realización de este documento, y así poder obtener conclusiones de las posibles diferencias. Hay que señalar que los modelos experimentales no forman parte de este estudio. Los ensayos en pilares fueron realizados en sección cuadrada y circular evaluando la rotura a compresión de las piezas, habiendo sido éstas escaladas con un factor de reducción de 2,3. Los ensayos correspondientes a vigas se realizaron en sección rectangular, centrándose en la evaluación de la rotura a flexión y habiendo sido escaladas igualmente, pero con un factor de reducción de 1:2. El documento se estructura en cuatro capítulos, cuyo contenido se expone de forma concisa a continuación. En el capítulo uno o marco teórico se exponen los principios de comportamiento y tipologías de los pilares y vigas de hormigón armado, las bases teóricas de su refuerzo y confinamiento, así como las diversas técnicas de refuerzo existentes. Se detalla la técnica con FRP, comparando y analizando sus ventajas e inconvenientes. En el capítulo dos se expone el proceso de fabricación, refuerzo y resultados de los modelos experimentales realizados para ambos elementos estructurales. La obtención de los modelos teóricos forma parte del capítulo tres, comparándose con los resultados experimentales en el cuarto capítulo. Finalmente, en el último capítulo se presentan las conclusiones obtenidas al realizar esta comparativa en el refuerzo de vigas y pilares con fibra de carbono. This work refers to the theoretical and experimental study of the behavior of CFRP reinforced concrete columns and beams. The analysis was done considering that the pillars are reinforced by CFRP wrapping technique, resulting in a confinement effect. The beams are reinforced by the addition of bars of the same material, with shear reinforcements. The objective is to compare the analytical study of this type of reinforcement with experimental results obtained prior to the performance of this document, and draw conclusions for any differences. Notice that experimental models are not part of this study. The tests were performed on circular and square section pillars, evaluating compression fracture of the pieces, having been scaled down with a factor of 2.3. The tests were performed on rectangular section beams, focusing on evaluation of the bending fracture and being scaled down equally, but with a factor of 1:2. The document is divided into four chapters, whose content is set out concisely below. The chapter one or theoretical framework sets out the principles of behavior and types of columns and beams of reinforced concrete, the theoretical basis of its reinforcement and confinement, as well as various existing reinforcement techniques. CFRP technique it’s detailed, comparing and analyzing their advantages and disadvantages. Chapter two describes the process of manufacture, reinforcement and results of experimental models made for both structural elements. Chapter three shows the obtaining of the theoretical models, comparing them with the experimental results in the fourth chapter. Finally, the last chapter presents the conclusions to make this comparison in the strengthening of beams and columns with carbon fiber.

Análisis de los modelos de comportamiento de vigas de hormigón armado reforzadas a cortante con polímeros armados con fibras (frp). Validación y calibración experimental

Los polímeros armados con fibras (FRP) se utilizan en refuerzos de estructuras de hormigón debido sobre todo a sus excelentes propiedades mecánicas, su resistencia a la corrosión y a su ligereza que se traduce en facilidad y ahorro en el transporte, puesta en obra y aplicación, la cual se realiza de forma muy rápida, con pocos operarios y utilizando medios auxiliares ligeros, minimizándose las interrupciones del uso de la estructura y las molestias a los usuarios. Las razones presentadas anteriormente, han despertado un gran inter´es por parte de diferentes grupos de investigación a nivel mundial y que actualmente se encuentran desarrollando nuevas técnicas de aplicación y métodos de cálculo. Sin embargo, las investigaciones realizadas hasta la fecha, muestran un procedimiento bien definido y aceptado en lo referente al cálculo a flexión, lo cual no ocurre con el refuerzo a cortante y aunque se ha demostrado que el refuerzo con FRP es un sistema eficaz para incrementar la capacidad ´ultima frente a esfuerzos cortantes, también se pone de manifiesto la necesidad de más estudios experimentales y teóricos para avanzar en el entendimiento de los mecanismos involucrados para este tipo de refuerzo y establecer un procedimiento de diseño apropiado que maximice las excelentes propiedades de este material. Los modelos que explican el comportamiento del refuerzo a cortante de elementos de hormigón armado son complejos y sin transposición directa a fórmulas ingenieriles. Las normas actualmente en vigor, generalmente, establecen empíricamente la capacidad cortante como la suma de las capacidades del hormigón y el refuerzo transversal de acero. Cuando un elemento es reforzado externamente con FRP, los modelos son evidentemente aun más complejos. Las guías y recomendaciones existentes proponen calcular la capacidad del elemento añadiendo la resistencia aportada por el refuerzo externo de FRP a la ya dada por el hormigón y acero transversal. Sin embargo, la idoneidad de este acercamiento es cuestionable puesto que no tiene en cuenta una posible interacción entre refuerzos. Con base en lo anterior se da origen al tema objeto de este trabajo, el cual está orientado al estudio a cortante de elementos de hormigón armado (HA), reforzados externamente con material compuesto de tejido unidireccional de fibra de carbono y resina epoxi. Inicialmente se hace una completa revisión del estado actual del conocimiento de la resistencia a cortante en elementos de hormigón armado con y sin refuerzo externo de FRP, prestando especial atención en los mecanismos actuantes estudiados hasta la fecha. La bibliografía consultada ha sido exhaustiva y actualizada lo que ha permitido el estudio de los modelos propuestos más importantes, tanto para la descripción del fenómeno de adherencia entre hormigón-FRP como de la valoración del aporte al cortante total hecho por el FRP, a través de sendas bases de datos de ensayos de pull-out y de vigas de hormigón armado ensayadas a cortante. Con base en todo lo anterior, se expusieron los mecanismos actuantes en el aporte a cortante hecho por el FRP en elementos de hormigón armado y la forma como las principales guías de cálculo existentes hasta la fecha los abordan. De igual forma se define un modelo de resistencia de esfuerzos para el FRP y se proponen dos modelos para el cálculo de las tensiones o deformaciones efectivas, de los cuales uno esta basado en el modelo de adherencia propuesto por Oller (2005) y el otro en una regresión multivariante para los mecanismos expuestos. Como complemento del estudio de los trabajos encontrados en la literatura, se lleva acabo un programa experimental que, además de aportar más registros a la exigua base de datos existentes, aporte mayor luz a los puntos que se consideran están deficientemente resueltos. Dentro de este programa se realizaron 32 ensayos sobre 16 vigas de 4.5 m de longitud (dos ensayos por viga), reforzadas a cortante con tejido unidireccional de CFRP. Finalmente, estos estudios han permitido proponer modificaciones a las formulaciones existentes en los códigos y guías en vigor. Abstract Its excellent mechanical properties, as well as its corrosion resistance and light weight, which make it easy to apply and inexpensive to ship to the worksite, are the basis of the extended use of fiber reinforced polymer (FRP) as external strengthening for structures. FRP strengthening is a rapid operation calling for only limited labor and lightweight ancillary equipment, all of which minimizes both the interruption of facility usage and user inconvenience. These advantages have aroused considerable interest in civil engineering science and technology and have led to countless applications the world over. Research studies on the shear strength of FRP-strengthened members have been much fewer in number and more controversial than the research on flexural strengthening, for which a more or less standardized and generally accepted procedure has been established. The research conducted and a host of applications around the world have shown that FRP strengthening is an effective technique for raising ultimate shear strength, but it has also revealed a need for further experimental and theoretical research to advance in the understanding of the mechanisms involved and establish suitable design procedures that optimize the excellent properties of this material The models that explain reinforced concrete (RC) shear strength behavior are complex and cannot be directly transposed to engineering formulas. The standards presently in place generally establish shear capacity empirically as the sum of the capacities of the concrete and the passive reinforcement. When members are externally strengthened with FRP, the models are obviously even more complex. The existing guides and recommendations propose calculating capacity by adding the external strength provided by the FRP to the contributions of the concrete and passive reinforcement. The suitability of this approach is questionable, however, because it fails to consider the interaction between passive reinforcement and external strengthening. The subject of this work is based in above, which is focused on externally shear strengthening for reinforced concrete members with unidirectional carbon fiber sheets bonded with epoxy resin. v Initially a thorough literature review on shear of reinforced concrete beams with and without external FRP strengthening was performed, paying special attention to the acting mechanisms studied to date, which allowed the study of the most important models both to describe the bond phenomenon as well as calculating the FRP shear contribution, through separate databases of pull-out tests and shear tests on reinforced concrete beams externally strengthened with FRP. Based on above, they were exposed the acting mechanisms in a FRP shear strengthening on reinforced concrete beams and how guidelines deal the topic. The same way, it is defined a FRP stress strength model and two more models are proposed for calculating the effective stress, one of these is based on the Oller (2005) bond model and another one is the data best fit, taking into account most of the acting mechanisms. To complement the theoretical part we develop an experimental program that, in addition to providing more records to the meager existing database provide greater understanding to the points considered poorly resolved. The test program included 32 tests of 16 beams (2 per beam) of 4.5 m long, shear strengthened with FRP, externally. Finally, modifications to the existing codes and guidelines are proposed.

Analysis of the Fracture of Reinforced Concrete Flat Elements Subjected to Explosions. Experimental Procedure and Numerical Validation

Many of the material models most frequently used for the numerical simulation of the behavior of concrete when subjected to high strain rates have been originally developed for the simulation of ballistic impact. Therefore, they are plasticity-based models in which the compressive behavior is modeled in a complex way, while their tensile failure criterion is of a rather simpler nature. As concrete elements usually fail in tensión when subjected to blast loading, available concrete material models for high strain rates may not represent accurately their real behavior. In this research work an experimental program of reinforced concrete fíat elements subjected to blast load is presented. Altogether four detonation tests are conducted, in which 12 slabs of two different concrete types are subjected to the same blast load. The results of the experimental program are then used for the development and adjustment of numerical tools needed in the modeling of concrete elements subjected to blast.

The ballast pick-up problem. A theoretical approach and two experimental campaigns

The aim of this contribution is to present a theoretical approach and two experimental campaigns (on wind tunnel and on the track) concerning the research work about the ballast train-induced-wind erosion (BTIWE) phenomenon. When a high speed train overpasses the critical speed, it produces a wind speed close to the track large enough to start the motion of the ballast elements, eventually leading to the rolling of the stones (Kwon and Park, 2006) and, if these stones get enough energy, they can jump and then initiate a saltation-like chain reaction, as found in the saltation processes of soil eolian erosion (Bagnold, 1941). The expelled stones can reach a height which is larger than the lowest parts of the train, striking them (and the track surroundings) producing considerable damage that should be avoided. There is not much published work about this phenomenon, in spite of the great interest that exists due to its relevant applications in increasing the maximum operative train speed. Particularly, the initiation of flight of ballast due to the pass of a high speed train has been studied by Kwon and Park (2006) by performing field and wind tunnel experiments.

An Experimental and Numerical Study of Ballistic Impacts on a Turbine Casing Material at Varying Temperatures

An experimental and numerical study of ballistic impacts on steel plates at various temperatures (700ºC, 400ºC and room temperature) has been carried out. The motivation for this work is the blade‐off event that may occur inside a jet engine turbine. However, as a first attempt to understand this complex loading process, a somewhat simpler approach is carried out in the present work. The material used in this study is the FV535 martensitic stainless steel, which is one of the most commonly used materials for turbine casings. Based on material test data, a Modified Johnson‐Cook (MJC) model was calibrated for numerical simulations using the LS‐DYNA explicit finite element code (see Figure 1). To check the mesh size sensitivity, 2D axisymmetric finite element models with three different mesh sizes and configurations were used for the various temperatures. Two fixed meshes with 64 and 128 elements over the 2mm thick plate and one mesh with 32 elements over the thickness with adaptive remeshing were used in the simulations. The formation of adiabatic shear bands in the perforation process has been found critical in order to achieve good results. Adiabatic shear bands are formed by the temperature rise due to the accumulation of plastic strain during impact (see Figure 2). The influence of the thermal softening in the plastic model has hence been analyzed for the room temperature impact tests, where the temperature gradient is highest

Research on intermediate band solar cells and development of experimental techniques for their characterization under concentrated illumination

Abstract This work is a contribution to the research and development of the intermediate band solar cell (IBSC), a high efficiency photovoltaic concept that features the advantages of both low and high bandgap solar cells. The resemblance with a low bandgap solar cell comes from the fact that the IBSC hosts an electronic energy band -the intermediate band (IB)- within the semiconductor bandgap. This IB allows the collection of sub-bandgap energy photons by means of two-step photon absorption processes, from the valence band (VB) to the IB and from there to the conduction band (CB). The exploitation of these low energy photons implies a more efficient use of the solar spectrum. The resemblance of the IBSC with a high bandgap solar cell is related to the preservation of the voltage: the open-circuit voltage (VOC) of an IBSC is not limited by any of the sub-bandgaps (involving the IB), but only by the fundamental bandgap (defined from the VB to the CB). Nevertheless, the presence of the IB allows new paths for electronic recombination and the performance of the IBSC is degraded at 1 sun operation conditions. A theoretical argument is presented regarding the need for the use of concentrated illumination in order to circumvent the degradation of the voltage derived from the increase in the recombi¬nation. This theory is supported by the experimental verification carried out with our novel characterization technique consisting of the acquisition of photogenerated current (IL)-VOC pairs under low temperature and concentrated light. Besides, at this stage of the IBSC research, several new IB materials are being engineered and our novel character¬ization tool can be very useful to provide feedback on their capability to perform as real IBSCs, verifying or disregarding the fulfillment of the “voltage preservation” principle. An analytical model has also been developed to assess the potential of quantum-dot (QD)-IBSCs. It is based on the calculation of band alignment of III-V alloyed heterojunc-tions, the estimation of the confined energy levels in a QD and the calculation of the de¬tailed balance efficiency. Several potentially useful QD materials have been identified, such as InAs/AlxGa1-xAs, InAs/GaxIn1-xP, InAs1-yNy/AlAsxSb1-x or InAs1-zNz/Alx[GayIn1-y]1-xP. Finally, a model for the analysis of the series resistance of a concentrator solar cell has also been developed to design and fabricate IBSCs adapted to 1,000 suns. Resumen Este trabajo contribuye a la investigación y al desarrollo de la célula solar de banda intermedia (IBSC), un concepto fotovoltaico de alta eficiencia que auna las ventajas de una célula solar de bajo y de alto gap. La IBSC se parece a una célula solar de bajo gap (o banda prohibida) en que la IBSC alberga una banda de energía -la banda intermedia (IB)-en el seno de la banda prohibida. Esta IB permite colectar fotones de energía inferior a la banda prohibida por medio de procesos de absorción de fotones en dos pasos, de la banda de valencia (VB) a la IB y de allí a la banda de conducción (CB). El aprovechamiento de estos fotones de baja energía conlleva un empleo más eficiente del espectro solar. La semejanza antre la IBSC y una célula solar de alto gap está relacionada con la preservación del voltaje: la tensión de circuito abierto (Vbc) de una IBSC no está limitada por ninguna de las fracciones en las que la IB divide a la banda prohibida, sino que está únicamente limitada por el ancho de banda fundamental del semiconductor (definido entre VB y CB). No obstante, la presencia de la IB posibilita nuevos caminos de recombinación electrónica, lo cual degrada el rendimiento de la IBSC a 1 sol. Este trabajo argumenta de forma teórica la necesidad de emplear luz concentrada para evitar compensar el aumento de la recom¬binación de la IBSC y evitar la degradación del voltage. Lo anterior se ha verificado experimentalmente por medio de nuestra novedosa técnica de caracterización consistente en la adquisicin de pares de corriente fotogenerada (IL)-VOG en concentración y a baja temperatura. En esta etapa de la investigación, se están desarrollando nuevos materiales de IB y nuestra herramienta de caracterizacin está siendo empleada para realimentar el proceso de fabricación, comprobando si los materiales tienen capacidad para operar como verdaderas IBSCs por medio de la verificación del principio de preservación del voltaje. También se ha desarrollado un modelo analítico para evaluar el potencial de IBSCs de puntos cuánticos. Dicho modelo está basado en el cálculo del alineamiento de bandas de energía en heterouniones de aleaciones de materiales III-V, en la estimación de la energía de los niveles confinados en un QD y en el cálculo de la eficiencia de balance detallado. Este modelo ha permitido identificar varios materiales de QDs potencialmente útiles como InAs/AlxGai_xAs, InAs/GaxIni_xP, InAsi_yNy/AlAsxSbi_x ó InAsi_zNz/Alx[GayIni_y]i_xP. Finalmente, también se ha desarrollado un modelado teórico para el análisis de la resistencia serie de una célula solar de concentración. Gracias a dicho modelo se han diseñado y fabricado IBSCs adaptadas a 1.000 soles.

Experimental and Analytical Study of Masonry Infill Reinforced Concrete Frames Retrofitted with Steel Braces

In the present work a seismic retrofitting technique is proposed for masonry infilled reinforced concrete frames based on the replacement of infill panels by K-bracing with vertical shear link. The performance of this technique is evaluated through experimental tests. A simplified numerical model for structural damage evaluation is also formulated according to the notions and principles of continuum damage mechanics. The proposed model is calibrated with the experimental results. The experimental results have shown an excellent energy dissipation capacity with the proposed technique. Likewise, the numerical predictions with the proposed model are in good agreement with experimental results.

Experimental comparison of FBARs and SMRs responsitivities to mass loadings

The utilisation of thin film technology to develop film bulk acoustic resonators (FBARs) and solidly mounted resonators (SMRs), offers great potential to outperform the sensitivity and minimum detection limit of gravimetric sensors. Up to now, the choice between FBARs and SMRs depends primarily on the users' ability to design and fabricate Bragg reflectors and/or membranes, because neither of these two types of resonators has been demonstrated to be superior to the other. In the work reported here, it is shown that identically designed FBARs and SMRs resonating at the same frequency exhibit different responsitivities, Rm, to mass loadings, being the FBARs more responsive than the SMRs. For the specific device design and resonant frequency (~2 GHz) of the resonators presented, FBARs' mass responsitivity is ~20% greater than that of SMRs, and although this value should not be taken as universal for all possible device designs, it clearly indicates that FBAR devices should be favoured over SMRs in gravimetric sensing applications.

Automatic-dependent surveillance-broadcast experimental deployment using system wide information management

This paper describes an automatic-dependent surveillance-broadcast (ADS-B) implementation for air-to-air and ground-based experimental surveillance within a prototype of a fully automated air traffic management (ATM) system, under a trajectory-based-operations paradigm. The system is built using an air-inclusive implementation of system wide information management (SWIM). This work describes the relations between airborne and ground surveillance (SURGND), the prototype surveillance systems, and their algorithms. System's performance is analyzed with simulated and real data. Results show that the proposed ADS-B implementation can fulfill the most demanding surveillance accuracy requirements.

Estudio numérico y experimental del efecto de tamaño en modo mixto de los paneles de yeso laminado y lana de roca

Los paneles sándwich de yeso laminado y lana de roca presentan una abundante patología de fisuración debida a flechas excesivas de forjados. Existe, por tanto, la necesidad de avanzar en la simulación y predicción de comportamiento bajo solicitaciones de tracción y cortante de ese tipo de paneles, a pesar de que en las aplicaciones habituales no tienen responsabilidad estructural. El comportamiento de este material puede ser considerado cuasi-frágil, y en base a ello en este trabajo ha sido estudiado haciendo uso de modelos de fisura cohesiva, cuya aplicación a otros materiales cuasifrágiles, como el hormigón, ha aportado resultados muy satisfactorios. En esta comunicación se presenta el trabajo realizado para estudiar el efecto del tamaño del elemento de yeso laminado y lana de roca en su comportamiento mecánico-resistente. Para ello se diseñó una campaña de ensayos en modo mixto sobre probetas de diferente tamaño. Se han realizado ensayos de flexión en tres puntos en modo mixto de unas probetas entalladas, geométricamente similares y de diferente tamaño, obteniéndose las curvas carga-desplazamiento y cargaabertura de la boca de la entalla. Para simular numéricamente el comportamiento en fractura del panel en modo mixto se ha utilizado un modelo de elementos finitos con fisura embebida basado en la fisura cohesiva en el que se introducen como entrada los parámetros obtenidos a partir de la experimentación de trabajos anteriores, obteniéndose un buen ajuste. En función de estos resultados se analiza el efecto del tamaño en los paneles. Sandwich panels of laminated gypsum and rockwool have an abundant pathology of cracking due to excessive slabs deflection. Therefore, it is necessary to progress in the simulation and prediction of behaviour under tensile and shear load of such panels, although in typical applications have no structural responsability. The behaviour of this material may be considered quasi-brittle and, based on this idea, in this work has been studied using a cohesive crack model that has been applied to other quasi-brittle materials, such as concrete, and has provided very satisfactory results. This communication presents the work carried out to study the size effect of the specimen of plasterboard and rockwool in its mechanical and resistant behaviour. The authors designed an experimental campaign under mixed mode composed by testing specimens of different sizes. Assymetrical three-point bending tests have been performed on notched specimens, geometrically similar and of different size, to obtain load-displacement and load-crack moutn opening displacement curves. To numerically simulate the mixed-mode fracture behaviour of the panels we have used a finite element model with embedded crack, based on the cohesive crack model, using as input the experimental parameters obtained in previous work, obtaining a good adjustment. Based on these results we analyze the size effect of the panels

Experimental results on advanced inertial fusion schemes obtained within the HiPER project

This paper presents de results of experiments conducted within the Work Package 10 (fusion experimental programme) of the HiPER project. The aim of these experiments was to study the physics relevant for advanced ignition schemes for inertial confinement fusion, i.e. the fast ignition and the shock ignition. Such schemes allow to achieve a higher fusion gain compared to the indirect drive approach adopted in the National Ignition Facility in United States, which is important for the future inertial fusion energy reactors and for realising the inertial fusion with smaller facilities