Lipid extractions from marine deep-sea invertebrates : modifications to the Bligh & Dyer method to increase lipid yields

The deep-sea environment is difficult to sample, and often only small quantities of samples can be obtained when using less destructive methods than dredging. When working with marine animals that are difficult to sample and with limited quantities of tissue to extract lipids, it is essential to ensure that the used method extracts the maximum possible quantity of lipids. This study evaluates the efficiency of introducing modifications to the method originally described by Bligh & Dyer (1959). This lipid extraction method is broadly used with modifications, although these usually lack proper description and evaluation of increment in lipids. In this study we consider the improvement in terms of amount of lipids extracted by changing the method. Lipid content was determined by gravimetric measurements in eight invertebrates from the deep-sea, including deep-sea hydrothermal vents animals, using three different approaches. Results show increases of 14% to 30% in lipid contents obtained from hydrothermal vent invertebrate tissues and whole animals by placing the samples in methanol for 24 hours before applying the Bligh & Dyer mixture. Efficiency of the extractions using frozen and freeze-dried samples was also compared. For large sponges, the use of lyophilized materials resulted in increases of 3 to 7 times more lipids extracted when compared with extractions using frozen samples.

The flipped-broadcast learning system, application on Azores

On this work we suggest a teaching solution that can be implemented in Azores, an archipelago of nine islands of Portugal, based in already known system of e-learning, with a twist based on the flipped method. Structured in a cooperative way, the organization of the system allows to isolated groups of people to have access to a certain level of teaching, if they cannot have the possibility to have physical presence in school due to problems emerging from territory discontinuity. Our suggestion can be elaborate in a model that can be adapted to any level of education, and can be adapted also in cases that a cut of budget exists. We suggest the name of fb-learning: Flipped Broadcast Learning.