Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women. METHODS: In all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and CD38 (Bm1-Bm5 classification system). Regulatory B cells were characterized based on the expression of surface antigens (CD24, CD27, and CD38) and the production of IL-10 after lipopolysaccharide stimulation. RESULTS: Compared to the absolute counts of B cells in the non-pregnant women (n = 35), those in the pregnant women (n = 43) were significantly lower (p < 0.05) during the 3rd trimester of pregnancy and on delivery day (immediately after delivery). The percentages of these cells on delivery day and at post-partum were significantly lower than those in the non-pregnant women. In general, the absolute counts and percentages of the majority of the B cell subsets were significantly lower in the 3rd trimester of pregnancy and on delivery day than in the non-pregnant women. However, these counts and percentages did not differ significantly between the post-partum and the non-pregnant women. The most notable exceptions to the above were the percentages of naïve B cells (which were significantly higher in the 3rd trimester and on delivery day than in the non-pregnant women) and of CD24(hi)CD38(hi) regulatory B cells (which were significantly higher in the post-partum than in the non-pregnant women). CONCLUSION: According to our study, the peripheral B cell compartment undergoes quantitative changes during normal late pregnancy and post-partum. Such findings may allow us to better understand immunomodulation during human pregnancy and provide evidence that could aid in the development of new strategies to diagnose and treat pregnancy-associated disturbances. Our findings could also be useful for studies of the mechanisms of maternal responses to vaccination and infection.

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

Abstract BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women. METHODS: In all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and CD38 (Bm1-Bm5 classification system). Regulatory B cells were characterized based on the expression of surface antigens (CD24, CD27, and CD38) and the production of IL-10 after lipopolysaccharide stimulation. RESULTS: Compared to the absolute counts of B cells in the non-pregnant women (n = 35), those in the pregnant women (n = 43) were significantly lower (p < 0.05) during the 3rd trimester of pregnancy and on delivery day (immediately after delivery). The percentages of these cells on delivery day and at post-partum were significantly lower than those in the non-pregnant women. In general, the absolute counts and percentages of the majority of the B cell subsets were significantly lower in the 3rd trimester of pregnancy and on delivery day than in the non-pregnant women. However, these counts and percentages did not differ significantly between the post-partum and the non-pregnant women. The most notable exceptions to the above were the percentages of naïve B cells (which were significantly higher in the 3rd trimester and on delivery day than in the non-pregnant women) and of CD24(hi)CD38(hi) regulatory B cells (which were significantly higher in the post-partum than in the non-pregnant women). CONCLUSION: According to our study, the peripheral B cell compartment undergoes quantitative changes during normal late pregnancy and post-partum. Such findings may allow us to better understand immunomodulation during human pregnancy and provide evidence that could aid in the development of new strategies to diagnose and treat pregnancy-associated disturbances. Our findings could also be useful for studies of the mechanisms of maternal responses to vaccination and infection.

Identificação e Caraterização de Infraestruturas Críticas – Uma Metodologia

A proteção das Infraestruturas Críticas tornou-se numa questão essencial no sistema internacional e nos Estados. Mais recentemente, Portugal começou a acompanhar esta tendência. Neste debate, torna-se de crucial importância, a identificação das infraestruturas que devem ser consideradas como críticas. Esta identificação terá como principal objetivo a redução das suas vulnerabilidades e a eficiência no emprego de recursos para a proteção das mesmas. Mas que critérios e indicadores, em cada setor/subsetor, possibilitam uma adequada metodologia para a identificação e caraterização das Infraestruturas Críticas em Portugal? Com vista a responder a esta problemática será analisada a metodologia adotada por Portugal, bem como as componentes da metodologia de identificação e caraterização de Infraestruturas Críticas utilizadas em países e organizações de referência. Esta investigação tem como objetivo geral identificar de áreas de melhoria na metodologia adotada pela Autoridade Nacional de Proteção Civil e, com base na análise da metodologia usada em organizações e países de referência, contribuir para a identificação e caraterização das IC em Portugal. Conclui-se que a Identificação e Caraterização de Infraestruturas Críticas nacionais deve ser aplicada na primeira fase do processo de elaboração do Programa Nacional de Proteção de Infraestruturas Críticas, apresentando, simultaneamente, uma definição de Infraestrutura Crítica, através de possíveis agrupamentos em setores, critérios e indicadores a adotar. Abstract: Critical infrastructure protection has become a key issue for states in the international system. Recently, Portugal has joined this trend. In this debate, the identification of structures to be considered critical infrastructure becomes crucial. This process of identification should have as key purpose the reduction of these infrastructures, and an efficient use of resources in protecting them. However, which criteria and indicators, for each sector/ sub-sector, allow for an adequate methodology for identifying and characterizing critical infrastructures in Portugal? In order to answer this, this research will analyse the methodology adopted by the National Civil Protection Authority, as well as some methodology components for identifying and characterizing critical infrastructure used by reference countries and organizations. The main purpose of this research is thus to contribute to the development of a methodology to be used in Portugal, through the development of criteria and indicators that prove adequate to identifying and characterizing Portuguese critical infrastructure. It concludes that the identification and characterization of national critical infrastructures should be applied in the first phase of elaborating a national program for the protection of critical infrastructures, while simultaneously presenting a definition of critical infrastructure, through possible grouping in sectors, criteria and indicators to adopt.