Relatório Final de Estágio em Enfermagem Comunitária

O presente Relatório está contextualizado no plano curricular de estágio do 1º Mestrado em Enfermagem da Escola Superior de Saúde de Portalegre - Instituto Politécnico de Portalegre, na Área de Especialização em Enfermagem Comunitária, que decorreu entre 14 de Fevereiro a 30 de Junho de 2011. O estágio foi dividido em duas áreas de intervenção comunitárias distintas: a primeira na área da educação sexual na adolescência que se realizou na Escola Secundária Mouzinho da Silveira e na Escola Secundária de São Lourenço, surgindo como resposta a um projeto trabalho conjunto entre esta escola e a Escola Superior de Saúde de Portalegre, onde foi realizado por nós um diagnóstico de situação. A segunda intervenção surge na área da promoção da imagem da Escola Superior de Saúde de Portalegre, que visou facilitar as escolhas da vida profissional através da promoção da imagem da escola e do conhecimento da oferta formativa nela existente junto dos alunos do 9º e 12º anos do concelho de Portalegre e na informação para o desenvolvimento e promoção de hábitos de vida saudáveis por parte dos jovens e ainda a demonstração da execução do suporte básico de vida. A população alvo de intervenção foram os alunos do 8º,9º 10º, entre os 13 e os 22 anos, num total de 693 jovens. Os objetivos do estágio foram: adquirir competências tendo por base a metodologia do planeamento em saúde, na avaliação do estado de saúde desta comunidade; bem como: Contribuir para o processo de capacitação desta comunidade. Conhecer os comportamentos e os estilos de vida dos adolescentes, integrados no sistema educativo. Em relação ao relatório de estagio o principal objetivo foi: encontrar respostas honestas e proceder a uma auto-avaliação construtiva e de aprendizagem pessoal (Soares et al 1997: 36)

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women. METHODS: In all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and CD38 (Bm1-Bm5 classification system). Regulatory B cells were characterized based on the expression of surface antigens (CD24, CD27, and CD38) and the production of IL-10 after lipopolysaccharide stimulation. RESULTS: Compared to the absolute counts of B cells in the non-pregnant women (n = 35), those in the pregnant women (n = 43) were significantly lower (p < 0.05) during the 3rd trimester of pregnancy and on delivery day (immediately after delivery). The percentages of these cells on delivery day and at post-partum were significantly lower than those in the non-pregnant women. In general, the absolute counts and percentages of the majority of the B cell subsets were significantly lower in the 3rd trimester of pregnancy and on delivery day than in the non-pregnant women. However, these counts and percentages did not differ significantly between the post-partum and the non-pregnant women. The most notable exceptions to the above were the percentages of naïve B cells (which were significantly higher in the 3rd trimester and on delivery day than in the non-pregnant women) and of CD24(hi)CD38(hi) regulatory B cells (which were significantly higher in the post-partum than in the non-pregnant women). CONCLUSION: According to our study, the peripheral B cell compartment undergoes quantitative changes during normal late pregnancy and post-partum. Such findings may allow us to better understand immunomodulation during human pregnancy and provide evidence that could aid in the development of new strategies to diagnose and treat pregnancy-associated disturbances. Our findings could also be useful for studies of the mechanisms of maternal responses to vaccination and infection.

Characterization of B cells in healthy pregnant women from late pregnancy to post-partum: a prospective observational study

Abstract BACKGROUND: B cells play a role in pregnancy due to their humoral and regulatory activities. To our knowledge, different maturational stages (from transitional to memory) of circulating B cell subsets have not yet been characterized (cell quantification and phenotype identification) in healthy pregnant women. Thus, the objective of our study was to characterize these subsets (as well as regulatory B cells) from late pregnancy to post-partum and to compare them with the circulating B cells of non-pregnant women. METHODS: In all of the enrolled women, flow cytometry was used to characterize the circulating B cell subsets according to the expression of IgD and CD38 (Bm1-Bm5 classification system). Regulatory B cells were characterized based on the expression of surface antigens (CD24, CD27, and CD38) and the production of IL-10 after lipopolysaccharide stimulation. RESULTS: Compared to the absolute counts of B cells in the non-pregnant women (n = 35), those in the pregnant women (n = 43) were significantly lower (p < 0.05) during the 3rd trimester of pregnancy and on delivery day (immediately after delivery). The percentages of these cells on delivery day and at post-partum were significantly lower than those in the non-pregnant women. In general, the absolute counts and percentages of the majority of the B cell subsets were significantly lower in the 3rd trimester of pregnancy and on delivery day than in the non-pregnant women. However, these counts and percentages did not differ significantly between the post-partum and the non-pregnant women. The most notable exceptions to the above were the percentages of naïve B cells (which were significantly higher in the 3rd trimester and on delivery day than in the non-pregnant women) and of CD24(hi)CD38(hi) regulatory B cells (which were significantly higher in the post-partum than in the non-pregnant women). CONCLUSION: According to our study, the peripheral B cell compartment undergoes quantitative changes during normal late pregnancy and post-partum. Such findings may allow us to better understand immunomodulation during human pregnancy and provide evidence that could aid in the development of new strategies to diagnose and treat pregnancy-associated disturbances. Our findings could also be useful for studies of the mechanisms of maternal responses to vaccination and infection.

The medico-legal importance of establishing human identity by palatal rugoscopy: evaluation of the immutability and individuality of palatal rugae under the influence of ante mortem orthodontic treatment

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