1 resultado para action and adventure films
em Instituto Nacional de Saúde de Portugal
Filtro por publicador
- Repository Napier (1)
- University of Cagliari UniCA Eprints (1)
- Abertay Research Collections - Abertay University’s repository (1)
- Aberystwyth University Repository - Reino Unido (5)
- Academic Archive On-line (Karlstad University; Sweden) (1)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (10)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (4)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (6)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (4)
- Aston University Research Archive (31)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Câmara dos Deputados (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (19)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (12)
- Biblioteca Digital de la Universidad Católica Argentina (4)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (2)
- Biodiversity Heritage Library, United States (1)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (29)
- Boston University Digital Common (1)
- Brock University, Canada (7)
- Bucknell University Digital Commons - Pensilvania - USA (2)
- CaltechTHESIS (3)
- Cambridge University Engineering Department Publications Database (10)
- CentAUR: Central Archive University of Reading - UK (33)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (31)
- Cochin University of Science & Technology (CUSAT), India (8)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (14)
- CORA - Cork Open Research Archive - University College Cork - Ireland (5)
- Dalarna University College Electronic Archive (3)
- Deakin Research Online - Australia (67)
- Department of Computer Science E-Repository - King's College London, Strand, London (1)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (2)
- Digital Commons at Florida International University (9)
- Digital Peer Publishing (1)
- DigitalCommons - The University of Maine Research (2)
- DigitalCommons@The Texas Medical Center (10)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (4)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (5)
- Harvard University (1)
- Helda - Digital Repository of University of Helsinki (32)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (3)
- Indian Institute of Science - Bangalore - Índia (58)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (2)
- Instituto Nacional de Saúde de Portugal (1)
- Instituto Politécnico de Bragança (2)
- Instituto Politécnico de Castelo Branco - Portugal (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (4)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Massachusetts Institute of Technology (2)
- Ministerio de Cultura, Spain (1)
- National Center for Biotechnology Information - NCBI (11)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (1)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- Publishing Network for Geoscientific & Environmental Data (3)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (53)
- Queensland University of Technology - ePrints Archive (154)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Digital da Universidade Municipal de São Caetano do Sul - USCS (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (101)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad de Alicante (4)
- Universidad del Rosario, Colombia (6)
- Universidad Politécnica de Madrid (6)
- Universidade de Lisboa - Repositório Aberto (4)
- Universita di Parma (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (6)
- Université Laval Mémoires et thèses électroniques (1)
- University of Michigan (54)
- University of Queensland eSpace - Australia (16)
- University of Washington (4)
- WestminsterResearch - UK (1)
Resumo:
The intestinal mucosa is the first biological barrier encountered by natural toxins, and could possibly be exposed to high amounts of dietary mycotoxins. Patulin (PAT), a mycotoxin produced by Penicillium spp. during fruit spoilage, is one of the best known enteropathogenic mycotoxins able to alter functions of the intestine (Maresca et al., 2008). This study evaluated the effects of PAT on barrier function of the gut mucosa utilizing the intestinal epithelial cell model Caco-2, and scrutinized immunomodulatory effects using human peripheral blood mononuclear cells (PBMC) and human blood monocyte-derived dendritic cells (moDCs) as test systems. PAT exposure reduced Caco-2 cell viability at concentrations above 12 mM. As expected, the integrity of a polarized Caco-2 monolayer was affected by PAT exposure, as demonstrated by a decrease in TER values, becoming more pronounced at 50 mM. No effects were detected on the expression levels of the tight junction proteins occludin, claudin-1 and claudin-3 at 50 mM. However, the expression of zonula occludens-1 (ZO-1) and myosin light chain 2 (MLC2) declined. Also, levels of phospho-MLC2 (p-MLC2) increased after 24 h of exposure to 50 mM of PAT. T cell proliferation was highly sensitive to PAT with major effects for concentrations above 10 nM of PAT. The same conditions did not affect the maturation of moDC. PAT causes a reduction in Caco-2 barrier function mainly by perturbation of ZO-1 levels and the phosphorylation of MLC. Low doses of PAT strongly inhibited T cell proliferation induced by a polyclonal activator, but had no effect on the maturation of moDC. These results provide new information that strengthens the concept that the epithelium and immune cells of the intestinal mucosa are important targets for the toxic effects of food contaminants like mycotoxins