DNA barcoding: an effective tool to overcome morphological identification constraints in the assessment of the ecological quality


Autoria(s): Medeiros, J.P.; Weisberg, S. B.; Alves, M.J.; Stein, E.; Gillett, D.; Chainho, P.; Costa, J.L.; Adão, H; Costa, M:J
Data(s)

06/01/2017

06/01/2017

17/10/2016

Resumo

DNA barcoding has the potential to overcome taxonomic challenges in biological community assessments. However, fulfilling that potential requires successful amplification of a large and unbiased portion of the community. In this study, we attempted to identify mitochondrial gene cytochrome c oxidase I (COI) barcodes from 1024 benthic invertebrate specimens belonging to 54 taxa from low salinity environments of the Mira estuary and Torgal riverside (SW Portugal). Up to 17 primer pairs and several reaction conditions were attempted among specimens from all taxa, with amplification success defined as a single band of approximately 658 bp visualized on a pre-cast agarose gel, starting near the 5' end of the COI gene and suitable for sequencing. Amplification success was achieved for 99.6% of the 54 taxa, though no single primer was successful for more than 88.9% of the taxa. However, only 68.5% of the specimens within these taxa successfully amplified. Inhibition factors resulting from a non-purified DNA extracted and inexistence of species-specific primers for COI were pointed as the main reasons for an unsuccessful amplification. These results suggest that DNA barcoding can be an effective tool for application in low salinity environments where taxa such as chironomids and oligochaetes are challenging for morphological identification. Nevertheless, its implementation is not simple, as methods are still being standardized and multiple species

Identificador

Medeiros, J.P., Stephen B. Weisberg S. B., AlvesM.J., Stein E., Gillett D., Chainho, P., Costa J.L., Adão H., Costa, M.J., 2016. DNA barcoding: an effective tool to overcome morphological identification constraints in the assessment of the ecological quality. Community Ecology for 21th Century (Genes to Ecosystems: Cibio InBio) Conference, 17-19 October, Evora, Portugal

http://hdl.handle.net/10174/19569

sim

nao

nao

nd

nd

nd

nd

nd

pmchainho@fc.ul.pt

jlcosta@fc.ul.pt

hadao@uevora.pt

nd

367

Idioma(s)

eng

Publicador

CIbio- Inbio: Community Ecology for 21th Century (Genes to Ecosystems)

Direitos

openAccess

Palavras-Chave #mitochondrial DNA #COI primers #aquatic ecosystems #benthic infauna #PCR #molecular method,
Tipo

lecture