Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF-7
Data(s) |
01/04/2016
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Resumo |
Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum (C. inophyllum) fruit extract against human breast cancer cells MCF-7. <br /><br />Methods: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays for 24 h and the morphological investigation of treated MCF-7 cells was observed under optical microscope using Giemsa staining. <br /><br />Results: The cytotoxic effect of C. inophyllum fruit extract against MCF-7 cancer cells was evaluated through MTT and CyQuant assays simultaneously for 24 h after treatment, which demonstrated the inhibition of cell viability with the IC50 values of 19.63 μg/mL and 27.54 μg/mL, respectively. The preliminary time-based morphological investigation of MCF-7 cells treated with the IC50 value (23.59 μg/mL) of C. inophyllum fruit extract was observed under an optical microscopy via Giemsa staining, which exhibited prominent histological characteristics of apoptosis. <br /><br />Conclusions: This study clearly proved that the proliferation of human breast cancer cell MCF-7 was inhibited by C. inophyllum fruit extract resulted from the induction of apoptosis in MCF-7 cells. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Hainan Medical College |
Relação |
http://dro.deakin.edu.au/eserv/DU:30085324/kanwar-effectsof-2016.pdf http://www.dx.doi.org/10.1016/S2222-1808(15)61033-7 |
Direitos |
2016, Asian Pacific Tropical Medicine Press |
Palavras-Chave | #antiproliferative activity #human breast cancer #CyQuant assays #Giemsa staining |
Tipo |
Journal Article |