Counter-pressure-assisted ITP with electrokinetic injection under field-amplified conditions for bacterial analysis
Data(s) |
01/09/2015
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Resumo |
Counter-pressure was used to extend the duration of field-amplified sample injection in isotachophoresis (FASI-ITP) in order to improve the detection of bacterial cells. Using 0.51-μm negatively charged encapsulated fluorescent beads as a model, the counter-pressure, injection and separation voltages, and times were optimized. Using 6-min 8,963-Pa counter-pressure FASI-ITP injections at −12 kV followed by mobilization of the ITP band with continued injection at −6 kV, the limit of detection (LOD) for Escherichia coli was improved to 78 cells/mL, a factor of 4 when compared with FASI-ITP without counter-pressure. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer |
Relação |
http://dro.deakin.edu.au/eserv/DU:30084956/phung-counterpressure-2015.pdf http://www.dx.doi.org/10.1007/s00216-015-8838-4 |
Direitos |
2015, Springer |
Palavras-Chave | #capillary electrophoresis/electrophoresis #cell systems/single cell analysis #counter-pressure #capillary isotachophoresis #microbials #eletrokinetic injections #field amplified |
Tipo |
Journal Article |