Evaluation of an in-house molecular HIV-1 test to assess mother-to-child HIV-1 transmission in Angola (the APEHC cohort)


Autoria(s): Martin, F.; Palladino, C.; Mateus, R.; Clemente, S.; Gomes, P.; Taveira, N.
Data(s)

12/10/2016

12/10/2016

27/09/2015

Resumo

Poster presented at the 17th Annual International Meeting of the Institute of Human Virology. Baltimore, 27-30 September 2015

"Mother-to-child-transmission (MTCT) rate has decreased sharply in recent years in most of the sub-Saharan Africa, however 220,000 children acquired HIV-1 in 2014. PCR detection of proviral DNA is the most sensitive method for early infant diagnosis (EID) of HIV-1 infection. Commercial kits are available but they have poor sensitivity with divergent non-B subtypes and high costs (≈30€ per test) which limit their use in resource-limited settings. The HIV-1 epidemic in Angola is driven by highly divergent strains of all group M subtypes, except B, as well as multiple recombinant forms (CRFs and URFs) making EID a challenge in this setting. The aim of this study was to develop and validate a qualitative, inexpensive and sensitive “inhouse” HIV-1 EID assay on heel prick dried blood spots (DBS) from infants of the Hospital da Divina Providência (HDP) in Luanda, Angola and determine the current HIV-1 MTCT rate in the Angolan PErinatal HIV Cohort (APEHC)."

Fundação para a Ciência e a Tecnologia - Portugal ; European Union

Identificador

http://hdl.handle.net/10400.26/15090

Idioma(s)

eng

Relação

info:eu-repo/grantAgreement/FCT/3599-PPCDT/122400/PT

info:eu-repo/grantAgreement/FCT/3599-PPCDT/123654/PT

info:eu-repo/grantAgreement/FCT/3599-PPCDT/115290/PT

info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87488%2F2012/PT

Direitos

openAccess

Palavras-Chave #Molecular HIV-1 test #In-house #Mother-to-child HIV-1 transmission #Angola
Tipo

conferenceObject