Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom


Autoria(s): Vinhote, J. F. C.; Torres, A. F. C.; Dantas, R. T.; Praciano, T. P.; Menezes, R. R. P. P. B.; Sousa, D. F.; Brito, T. S.; Lima, F. J. B.; Toyama, M. H.; Magalhaes, P. J.; Monteiro, H. S. A.; Martins-Nunes, A. M. C.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

25/10/2016

20/05/2014

25/10/2016

01/01/2011

Resumo

In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 mu g/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic rings maintained under basal tension. This contractile response was inhibited when preparations were maintained in Ca2+-free medium. Likewise, verapamil, a voltage-gated calcium channel blocker, also inhibited the contractile response. In this study, phentolamine, a blocker of a-adrenergic receptor blocker, significantly reduced the contractile effect of PPV in the aortic ring. In conclusion, PPV produced nephrotoxicity, which suggests a direct effect on necrotic cellular death in renal tubule cells. The vascular contractile effect of PPV appears to involve calcium influx through voltage-gated calcium channels via adrenergic regulation.

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificador

Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 2, p. 199-208, 2011.

1678-9199

http://hdl.handle.net/11449/347

http://acervodigital.unesp.br/handle/11449/347

S1678-91992011000200011

WOS:000290866500011

S1678-91992011000200011-en.pdf

http://dx.doi.org/10.1590/S1678-91992011000200011

Idioma(s)

eng

Publicador

Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)

Relação

Journal of Venomous Animals and Toxins Including Tropical Diseases

Direitos

info:eu-repo/semantics/openAccess

Palavras-Chave #Polybia paulista #venom #kidney #aorta #MDCK
Tipo

outro