The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
Cobertura |
Origin of publication: Brazil |
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Data(s) |
06/07/2016
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Resumo |
Localised cutaneous leishmaniasis (LCL) is the most common form of cutaneous leishmaniasis characterised by single or multiple painless chronic ulcers, which commonly presents with secondary bacterial infection. Previous culture- based studies have found staphylococci, streptococci, and opportunistic pathogenic bacteria in LCL lesions, but there have been no comparisons to normal skin. In addition, this approach has strong bias for determining bacterial composition. The present study tested the hypothesis that bacterial communities in LCL lesions differ from those found on healthy skin (HS). Using a high throughput amplicon sequencing approach, which allows for better populational evaluation due to greater depth coverage and the Quantitative Insights Into Microbial Ecology pipeline, we compared the microbiological signature of LCL lesions with that of contralateral HS from the same individuals. Streptococcus , Staphylococcus , Fusobacterium and other strict or facultative anaerobic bacteria composed the LCL microbiome. Aerobic and facultative anaerobic bacteria found in HS, including environmental bacteria, were significantly decreased in LCL lesions (p < 0.01). This paper presents the first comprehensive microbiome identification from LCL lesions with next generation sequence methodology and shows a marked reduction of bacterial diversity in the lesions. |
Formato |
html |
Identificador | |
Idioma(s) |
en |
Publicador |
Fundação Oswaldo Cruz, Fiocruz |
Relação |
http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc |
Direitos |
Copyright - 2016 Memórias do Instituto Oswaldo Cruz |
Fonte |
Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 111 Num 4 |
Palavras-Chave | #microbiome; LCL; wound; healthy skin; high-throughput sequencing |
Tipo |
AA |