Structural and Biochemical Characterization of Peroxiredoxin Q beta from Xylella fastidiosa CATALYTIC MECHANISM and HIGH REACTIVITY


Autoria(s): Horta, Bruno Brasil; de Oliveira, Marcos Antonio; Discola, Karen Fulan; Rosa Cussiol, Jose Renato; Soares Netto, Luis Eduardo
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

25/10/2016

20/05/2014

25/10/2016

21/05/2010

Resumo

The phytopathogenic bacterium Xylella fastidiosa is the etiological agent of various plant diseases. To survive under oxidative stress imposed by the host, microorganisms express antioxidant proteins, including cysteine-based peroxidases named peroxiredoxins. This work is a comprehensive analysis of the catalysis performed by PrxQ from X. fastidiosa (XfPrxQ) that belongs to a peroxiredoxin class still poorly characterized and previously considered as moderately reactive toward hydroperoxides. Contrary to these assumptions, our competitive kinetics studies have shown that the second-order rate constants of the peroxidase reactions of XfPrxQ with hydrogen peroxide and peroxynitrite are in the order of 107 and 106 M(-1) s(-1), respectively, which are as fast as the most efficient peroxidases. The XfPrxQ disulfides were only slightly reducible by dithiothreitol; therefore, the identification of a thioredoxin system as the probable biological reductant of XfPrxQ was a relevant finding. We also showed by site-specific mutagenesis and mass spectrometry that an intramolecular disulfide bond between Cys-47 and Cys-83 is generated during the catalytic cycle. Furthermore, we elucidated the crystal structure of XfPrxQ C47S in which Ser-47 and Cys-83 lie similar to 12.3 angstrom apart. Therefore, significant conformational changes are required for disulfide bond formation. In fact, circular dichroism data indicated that there was a significant redox-dependent unfolding of alpha-helices, which is probably triggered by the peroxidatic cysteine oxidation. Finally, we proposed a model that takes data from this work as well data as from the literature into account.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificador

Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 285, n. 21, p. 16051-16065, 2010.

0021-9258

http://hdl.handle.net/11449/311

http://acervodigital.unesp.br/handle/11449/311

10.1074/jbc.M109.094839

WOS:000277715900043

http://dx.doi.org/10.1074/jbc.M109.094839

Idioma(s)

eng

Publicador

Amer Soc Biochemistry Molecular Biology Inc

Relação

Journal of Biological Chemistry

Direitos

info:eu-repo/semantics/closedAccess

Tipo

outro