Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiabá, state of Mato Grosso, Brazil


Autoria(s): Serra, Otacília Pereira; Cardoso, Belgath Fernandes; Ribeiro, Ana Lúcia Maria; Santos, Fábio Alexandre Leal dos; Slhessarenko, Renata Dezengrini
Cobertura

Origin of publication: Brazil

Data(s)

10/07/2016

Resumo

This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (RT-PCR) for 11 flavivirus and five alphavirus. Positive pools were tested by single RT-PCR followed by nucleotide sequencing, by RT-PCR for E1 gene [Mayaro virus (MAYV)] and by inoculation in Vero cells (MAYV) or C6/36 cells (flaviviruses). One/171 Aedes aegypti was positive for dengue virus (DENV)-1, 12/403 Culex quinquefasciatus , and four/171 Ae. aegypti for MAYV, which was isolated from two pools containing two nonengorged females of Ae. aegypti and two of Cx. quinquefasciatus. DENV-4 was detected in 58/171 pools of Ae. aegytpi, 105/403 Cx. quinquefasciatus, two/five Psorophora sp., two/11 Psorophora varipes / Psorophora albigenu , one/one Sabethes chloropterus , two/five Culex bidens / Culex interfor , and one/one Aedes sp. DENV-4 was isolated from two pools containing three and 16 nonengorged Cx. quinquefasciatus females. Phylogenetic analysis revealed MAYV belongs to genotype L, clustering with human samples of the virus previously identified in the city. Cuiabá has biodiversity and ecosystem favourable for vector proliferation, representing a risk for arbovirus outbreaks.

Formato

html

Identificador

http://www.bioline.org.br/abstract?id=oc16002

Idioma(s)

en

Publicador

Fundação Oswaldo Cruz, Fiocruz

Relação

http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc

Direitos

Copyright 2016 - Memórias do Instituto Oswaldo Cruz

Fonte

Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 111 Num 1

Palavras-Chave #dengue virus; Mayaro virus; entomological surveillance
Tipo

AA