Leishmaniamamazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil


Autoria(s): Oliveira, Everton Falcão de; Casaril, Aline Etelvina; Mateus, Nathália Lopes Fontoura; Murat, Paula Guerra; Fernandes, Wagner Souza; Oshiro, Elisa Teruya; Oliveira, Alessandra Gutierrez de; Galati, Eunice Aparecida Bianchi
Cobertura

Origin of publication: Brazil

Data(s)

10/07/2016

Resumo

Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmania in wild female sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do Sul (Brazil) between October 2012-March 2014, and dissected to investigate flagellates and/or submitted to molecular analysis to detect Leishmania DNA. A total of 1,164 females (77.56% of which were Lutzomyia cruzi ) representing 11 species were investigated using molecular analysis; 126 specimens of Lu. cruzi were dissected and also submitted to molecular analysis. The infection rate based on the presence of Leishmania DNA considering all the sandfly species analysed was 0.69%; only Leishmania (Leishmania) amazonensis was identified in Lu. cruzi by the molecular analysis. The dissections were negative for flagellates. This is the first record of the presence of L. (L.) amazonensis DNA in Lu. cruzi, and the first record of this parasite in this area. These findings point to the need for further investigation into the possible role of this sandfly as vector of this parasite.

Formato

html

Identificador

http://www.bioline.org.br/abstract?id=oc15142

Idioma(s)

en

Publicador

Fundação Oswaldo Cruz, Fiocruz

Relação

http://memorias.ioc.fiocruz.br; http://www.bioline.org.br/oc

Direitos

Copyright 2015 - Memórias do Instituto Oswaldo Cruz

Fonte

Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 110 Num 8

Palavras-Chave #natural infection; Lutzomyia cruzi; Leishmania amazonensis; molecular biology; polymerase chain reaction; dissection
Tipo

AA