Anti-Helicobacter pylori activity and oxidative burst inhibition by the naphthoquinone 5-methoxy-3,4-dehydroxanthomegnin from Paepalanthus latipes


Autoria(s): Kitagawa, Rodrigo Rezende; Bonacorsi, Cibele; Fonseca, Luiz Marcos da; Vilegas, Wagner; Raddi, Maria Stella Gonçalves
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

25/10/2016

20/05/2014

25/10/2016

01/02/2012

Resumo

Helicobacter pylori is a bacterium recognized as the major cause of chronic gastritis and peptic ulcers. Infection by H. pylori induces inflammatory responses and pathological changes in the gastric microenvironment. The host Keywords: immune cells (especially neutrophils) release inflammatory mediators and large 5-methoxy-3,4-dehydroxanthomegnin amounts of reactive oxygen species (ROS), which are associated with an increased Helicobacter pyloririsk of developing gastric cancer. In this study, we evaluated the anti-H. pylori and oxidative burst antioxidantactivitiesofa1,4-naphthoquinone-5-methoxy-3,4-dehydroxanthomegnin. Paepalanthus latipes The antimicrobial activity was assessed using a spectrophotometric microdilution technique, and antioxidant activity was assessed by noting the effect of 5-methoxy3,4-dehydroxanthomegnin on the neutrophil oxidative burst using luminol-and lucigenin-amplified chemiluminescence. The results showed that 5-methoxy-3,4dehydroxanthomegnin is a potent anti-H. pylori compound (MIC 64 µg/mL and MBC 128 µg/mL) and a strong antioxidant. 5-Methoxy-3,4-dehydroxanthomegnin decreased luminol- and lucigenin-amplified chemiluminescence, with ED50 values of 1.58±0.09 µg/mL and 5.4±0.15 µg/mL, respectively, reflecting an inhibitory effect on the oxidative burst. These results indicate that 5-methoxy-3,4-dehydroxanthomegnin is a promising compound for the prevention and treatment of diseases caused by H. pylori infection, such as gastritis, peptic ulceration, and gastric cancer, because reactive oxygen intermediates are involved in the pathogenesis of gastric mucosal injury induced by H. pylori infections.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificador

Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 22, n. 1, p. 53-59, 2012.

0102-695X

http://hdl.handle.net/11449/290

http://acervodigital.unesp.br/handle/11449/290

10.1590/S0102-695X2011005000193

S0102-695X2012000100008

S0102-695X2012000100008.pdf

http://dx.doi.org/10.1590/S0102-695X2011005000193

Idioma(s)

eng

Publicador

Sociedade Brasileira de Farmacognosia

Relação

Revista Brasileira de Farmacognosia

Direitos

info:eu-repo/semantics/openAccess

Palavras-Chave #5-methoxy-3,4-dehydroxanthomegnin #Helicobacter pylori #oxidative burst #Paepalanthus latipes
Tipo

outro