Optimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extraction


Autoria(s): Heleno, Sandrina A.; Diz, Patrícia; Prieto, M.A.; Barros, Lillian; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R.
Data(s)

29/09/2016

29/09/2016

2016

Resumo

Ergosterol, a molecule with high commercial value, is the most abundant mycosterol in Agaricus bisporus L. To replace common conventional extraction techniques (e.g. Soxhlet), the present study reports the optimal ultrasound-assisted extraction conditions for ergosterol. After preliminary tests, the results showed that solvents, time and ultrasound power altered the extraction efficiency. Using response surface methodology, models were developed to investigate the favourable experimental conditions that maximize the extraction efficiency. All statistical criteria demonstrated the validity of the proposed models. Overall, ultrasound-assisted extraction with ethanol at 375 W during 15 min proved to be as efficient as the Soxhlet extraction, yielding 671.5 ± 0.5mg ergosterol/100 g dw. However, with n-hexane extracts with higher purity (mg ergosterol/g extract) were obtained. Finally, it was proposed for the removal of the saponification step, which simplifies the extraction process and makes it more feasible for its industrial transference.

The authors acknowledge FCT (Portugal) for financial support to CIMO (PEst-OE/AGUI0690/2014), Sandrina A. Heleno (SFRH/BPD/101413/2014) and L. Barros research contract, to FCT/MEC and FEDER under Programme PT2020 for financial support to LSRE (Project UID/EQU/50020/2013), and to QREN, ON2 and FEDER (Projects NORTE-07-0124-FEDER-000014 and NORTE-07-0162-FEDER-000050). Xunta de Galicia for financial support for the post-doctoral researcher of M.A. Prieto.

Identificador

Heleno, Sandrina A.; Diz, Patrícia; Prieto, M.A.; Barros, Lillian; Rodrigues, A.E.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2016) - Optimization of ultrasound-assisted extraction to obtain mycosterols from Agaricus bisporus L. by response surface methodology and comparison with conventional Soxhlet extraction. Food Chemistry. ISSN 0308-8146. 197, p. 1054-1063

0308-8146

http://hdl.handle.net/10198/13332

10.1016/j.foodchem.2015.11.108

Idioma(s)

eng

Publicador

Elsevier

Relação

info:eu-repo/grantAgreement/FCT/5876/135938/PT

SFRH/BPD/101413/2014

info:eu-repo/grantAgreement/FCT/5876/147286/PT

Direitos

openAccess

http://creativecommons.org/licenses/by/4.0/

Palavras-Chave #Agaricus #Chemical fractionation #Hexanes #Sterols #Ultrasonics
Tipo

article