Secretome analysis of Trichoderma atroviride T17 biocontrol of Guignardia citricarpa


Autoria(s): Lima, Fernanda Blauth de; Félix, Carina; Osório, Nádia; Alves, Artur; Vitorino, Rui; Domingues, Pedro; Correia, António; Ribeiro, Rute T. da Silva; Esteves, Ana Cristina
Data(s)

30/06/2016

01/08/2016

Resumo

The fungal species Guignardia citricarpa is an important pathogen in citriculture. Members of the fungal genus Trichoderma are recognized as biocontrol agents but studies on the interactions between both fungi are scarce. This study aimed to identify extracellular proteins secreted by Trichoderma atroviride T17 that are related to the control of G. citricarpa. Two-dimensional gel electrophoresis (2D) was used to study the patterns of proteins secreted by T. atroviride T17 in medium containing glucose (control) and in medium containing G. citricarpa GC3 inactivated mycelium. We identified 59 of the 116 spots differentially expressed (50.86%) by LC–MS/MS. Of these, we highlight the presence of glycoside hydrolases (CAZy families 3, 43, 54, 76 and 93), chitinase, mutanase, a-1,3-glucanase, a-1,2-mannosidase, carboxylic hydrolase ester, carbohydrate-binding module family 13, glucan 1,3-b-glucosidase, a-galactosidase and Neutral protease 2. These proteins are related to mycoparasitism processes, stimuli and therefore to the biological control of pathogens. The results obtained are in agreement with reports describing an increase in the secretion of proteins related to mycoparasitism and biological control and a reduction in the secretion of proteins related to the metabolism of Trichoderma species grown in the presence of the pathogen. Moreover, these results are pioneer in understanding T. atroviride interaction with G. citricarpa. For the first time, we identified potential candidate proteins that may have a role in the antagonism mechanism of G. citricarpa by T. atroviride T17. Thus our results shed a light into the molecular mechanisms that T. atroviride use to control G. citricarpa.

Identificador

1049-9644

http://hdl.handle.net/10773/15841

Idioma(s)

eng

Publicador

Elsevier

Relação

PTDC/AGR-PRO/2183/2014

UID/AMB/50017/2013

UID/BIM/04501/2013

UID/IC/00051/2013

PEst-C/QUI/UI0062/2013

IF/00835/2013

BPD/102572/2014

BD/97613/2013

http://dx.doi.org/10.1016/j.biocontrol.2016.04.009

Direitos

restrictedAccess

Palavras-Chave #Trichoderma atroviride #Guignardia citricarpa #Biological control #Proteomics #Pathogen antagonism
Tipo

article