Time-resolved fluorescence observation of di-tyrosine formation in horseradish peroxidase upon ultrasound treatment leading to enzyme inactivation


Autoria(s): Tsikrika, Konstantina; Lemos, M. Adília; Chu, Boon-Seang; Bremner, David H.; Hungerford, Graham
Contribuinte(s)

Abertay University. School of Science, Engineering and Technology

Abertay University

Data(s)

11/10/2016

11/10/2016

20/09/2016

19/09/2016

Resumo

The application of ultrasound to a solution can induce cavitional phenomena and generate high localised temperatures and pressures. These are dependent of the frequency used and have enabled ultrasound application in areas such as synthetic, green and food chemistry. High frequency (100 kHz to 1 MHz) in particular is promising in food chemistry as a means to inactivate enzymes, replacing the need to use periods of high temperature. A plant enzyme, horseradish peroxidase, was studied using time-resolved fluorescence techniques as a means to assess the effect of high frequency (378 kHz and 583 kHz) ultrasound treatment at equivalent acoustic powers. This uncovered the fluorescence emission from a newly formed species, attributed to the formation of di-tyrosine within the horseradish peroxidase structure caused by auto-oxidation, and linked to enzyme inactivation.

Identificador

Tsikrika, K. et al. 2016. Time-resolved fluorescence observation of di-tyrosine formation in horseradish peroxidase upon ultrasound treatment leading to enzyme inactivation. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 173: pp.324-327. doi: 10.1016/j.saa.2016.09.035

1386-1425 (print)

1873-3557 (online)

http://hdl.handle.net/10373/2460

https://dx.doi.org/10.1016/j.saa.2016.09.035

Idioma(s)

en

Publicador

Elsevier

Relação

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 173

Direitos

Attribution-NonCommercial-NoDerivatives 4.0 International

http://creativecommons.org/licenses/by-nc-nd/4.0/

This is the author accepted version of the manuscript, © 2016 Elsevier, which is under embargo until 20th September 2017

Palavras-Chave #High frequency ultrasound #Time-resolved emission spectrum #Decay associated spectra #Excitation-emission matrix #Metal catalysed oxidation
Tipo

Journal Article

published

peer-reviewed

accepted