On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli
| Contribuinte(s) |
University of Aberdeen, Medicine, Medical Sciences & Nutrition, Medical Sciences |
|---|---|
| Data(s) |
06/09/2016
06/09/2016
06/09/2016
|
| Resumo |
We thank Frans Bianchi and Franz Ho for assistance with molecular cloning, Tim Rasmussen for providing the pTRC-MscK plasmid, Andrew Robinson for providing the pBAD-mEos3.2 plasmid, Matthias Heinemann for assistance with the flow cytometry measurements, Paul Schavemaker for performing Smoldyn simulations and Michiel Punter for programming ImageJ plugins for PALM reconstructions and single-particle tracking. We thank Ian Booth for critical reading of the manuscript, and Christoffer Åberg and Matteo Gabba for valuable discussions. The authors would like to thank David Dryden and Marcel Reuter for performing preliminary experiments from which this work has been built. The work was funded by the EU FP7 ITN-network program NICHE. Peer reviewed Publisher PDF |
| Identificador |
van den Berg , J , Galbiati , H , Rasmussen , A , Miller , S & Poolman , B 2016 , ' On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli ' Scientific Reports , vol 6 , 32709 . , 10.1038/srep32709 2045-2322 PURE: 70218442 PURE UUID: 3f2fcc9e-929a-4401-93fb-46001c71c0d9 |
| Idioma(s) |
eng |
| Relação |
Scientific Reports |
| Direitos |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Palavras-Chave | #mechanosensitive channels #osmoregulation #super-resolution microscopy #lateral diffusion #quantitative biology #single-molecule analysis #R Medicine #R |
| Tipo |
Journal article |
