A statistical test vs. a validation experiment in Gene Expression Study

Report published in the Proceedings of the National Conference on "Education and Research in the Information Society", Plovdiv, May, 2014

Comparative CT Method compares the Ct value of one target gene to another using the formula called 2-ΔΔCT. To make this method valid, the efficiency of the target amplification (the gene of interest) and the efficiency of the reference amplification (the endogenous control) must be equal. In this article we propose to test statistical hypotheses instead to perform validation biological experiments when we want to show that the efficiencies of the target and endogenous control amplifications are approximately equal.

Association for the Development of the Information Society, Institute of Mathematics and Informatics Bulgarian Academy of Sciences, Plovdiv University "Paisii Hilendarski"