Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment


Autoria(s): Sandison, Mairi E.; Tveen Jensen, K.; Gesellchen, F.; Cooper, J.M.; Pitt, A.R.
Data(s)

07/10/2014

Resumo

Reversible phosphorylation plays a key role in numerous biological processes. Mass spectrometry-based approaches are commonly used to analyze protein phosphorylation, but such analysis is challenging, largely due to the low phosphorylation stoichiometry. Hence, a number of phosphopeptide enrichment strategies have been developed, including metal oxide affinity chromatography (MOAC). Here, we describe a new material for performing MOAC that employs a magnetite-doped polydimethylsiloxane (PDMS), that is suitable for the creation of microwell array and microfluidic systems to enable low volume, high throughput analysis. Incubation time and sample loading were explored and optimized and demonstrate that the embedded magnetite is able to enrich phosphopeptides. This substrate-based approach is rapid, straightforward and suitable for simultaneously performing multiple, low volume enrichments. © the Partner Organisations 2014.

Formato

application/pdf

Identificador

http://eprints.aston.ac.uk/23576/1/Magnetite_doped_polydimethylsiloxane_PDMS_.pdf

Sandison, Mairi E.; Tveen Jensen, K.; Gesellchen, F.; Cooper, J.M. and Pitt, A.R. (2014). Magnetite-doped polydimethylsiloxane (PDMS) for phosphopeptide enrichment. Analyst, 139 (19), pp. 4974-4981.

Relação

http://eprints.aston.ac.uk/23576/

Tipo

Article

PeerReviewed