A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk


Autoria(s): Le, X.; Datta, N.; Deeth, H. C.
Contribuinte(s)

A.G. Marangoni

Data(s)

01/01/2006

Resumo

A sensitive quantitative reversed-phase HPLC method is described for measuring bacterial proteolysis and proteinase activity in UHT milk. The analysis is performed on a TCA filtrate of the milk. The optimum concentration of TCA was found to be 4%; at lower concentrations, non-precipitated protein blocked the HPLC while higher concentrations yielded lower amounts of peptides. The method showed greater sensitivity and reproducibility than a fluorescamine-based method. Quantification of the HPLC method was achieved by use of an external dipeptide standard or a standard proteinase. (c) 2006 Elsevier Ltd. All rights reserved.

Identificador

http://espace.library.uq.edu.au/view/UQ:82818

Idioma(s)

eng

Publicador

Elsevier

Palavras-Chave #Food Science & Technology #C1 #290104 Other Food Sciences #670105 Dairy products
Tipo

Journal Article