A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk
Contribuinte(s) |
A.G. Marangoni |
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Data(s) |
01/01/2006
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Resumo |
A sensitive quantitative reversed-phase HPLC method is described for measuring bacterial proteolysis and proteinase activity in UHT milk. The analysis is performed on a TCA filtrate of the milk. The optimum concentration of TCA was found to be 4%; at lower concentrations, non-precipitated protein blocked the HPLC while higher concentrations yielded lower amounts of peptides. The method showed greater sensitivity and reproducibility than a fluorescamine-based method. Quantification of the HPLC method was achieved by use of an external dipeptide standard or a standard proteinase. (c) 2006 Elsevier Ltd. All rights reserved. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Palavras-Chave | #Food Science & Technology #C1 #290104 Other Food Sciences #670105 Dairy products |
Tipo |
Journal Article |