Inhibition of COXs and 5-LOX and activation of PPARs by Australian Clematis species (Ranunculaceae)
Data(s) |
01/01/2006
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Resumo |
The species of Clematis (Ranunculaceae) have been traditionally used for inflammatory conditions by indigenous Australians. We have previously reported that the ethanol extract of Clematis pickeringii inhibited COX-1. In this study, we examined the ethanol extracts and fractions of three Clematis species, Clematis pickeringii, Clematis glycinoides and Clematis microphylla, on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). We further examined the activating effects on the protein expression of peroxisome proliferator-activated receptor alpha (PPAR alpha) and gamma (PPAR-gamma) in HepG2 cells. The ethanol extracts of three Clematis species inhibited the activities of COX-1, COX-2 and 5-LOX in the different extents. The stem extract of Clematis pickeringii showed the highest inhibitory activities among the three species on COX-1, COX-2 and 5-LOX with the IC50 values of 73.5, 101.2 and 29.3 mu g/mL. One of its fractions also significantly elevated PPAR gamma expression by 173, 280 and 435% and PPAR gamma expression by 140, 228 and 296% at 4, 8 and 16 mu g/mL, respectively. (c) 2005 Elsevier Ireland Ltd. All rights reserved. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier Ireland Ltd |
Palavras-Chave | #anti-inflammatory activity #cyclooxygenase-1 #-2 #5-lipoxygenase #PPAR alpha #PPAR gamma #complementary medicine #Antiinflammatory Drugs #Inflammation #Expression #Plants #Mice #C1 #321399 Complementary/Alternative Medicine not elsewhere classified |
Tipo |
Journal Article |