Shortcomings of protein removal prior to high performance liquid chromatographic analysis - A case study using method development for BAY 11-7082
Contribuinte(s) |
G. Hopfgartner H. T. Karnes R. Bischoff |
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Data(s) |
01/01/2006
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Resumo |
During the analytical method development for BAY 11-7082 ((E)-3-[4-methylphenylsulfonyl]-2-propenenitrile), using HPLC-MS-MS and HPLC-UV, we observed that the protein removal process (both ultrafiltration and precipitation method using organic solvents) prior to HPLC brought about a significant reduction in the concentration of this compound. The use of a structurally similar internal standard, BAY 11-7085 ((E)-3-[4-t-butylphenylsulfonyl]-2-propenenitrile), was not effective in compensating for the loss of analyte as the extent of reduction was different to that of the analyte. We present here a systematic investigation of this problem and a new validated method for the determination of BAY 11-7082. (c) 2006 Elsevier B.V. All rights reserved. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier BV |
Palavras-Chave | #Biochemical Research Methods #Chemistry, Analytical #Bay 11-7082 #Bay 11-7085 #HPLC-MS-MS #Protein Precipitation #Ultrafiltration #Nf-kappa-b #Dendritic Cells #Plasma #Activation #C1 #250499 Analytical Chemistry not elsewhere classified #780103 Chemical sciences |
Tipo |
Journal Article |