Simultaneous determination of morphine, oxycodone, morphine-3-glucuronide, and noroxycodone concentrations in rat serum by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry
Contribuinte(s) |
D. K. Lloyd R. Bischoff |
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Data(s) |
01/01/2005
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Resumo |
An assay using high performance liquid chromatography (HPLC)-electrospray ionization-tandem mass spectrometry (ESI-MS-MS) was developed for simultaneously determining concentrations of morphine, oxycodone, morphine-3-glucuronide, and noroxycodone, in 50 mul samples of rat serum. Deuterated (d(3)) analogues of each compound were used as internal standards. Samples were treated with acetonitrile to precipitate plasma proteins: acetonitrile was removed from the supernatant by centrifugal evaporation before analysis. Limits of quantitation (ng/ml) and their between-day accuracy and precision (%deviation and %CV) were-morphine, 3.8 (4.3% and 7.6%); morphine-3-glucuronide, 5.0 (4.5% and 2.9%); oxycodone, 4.5 (0.4% and 9.3%); noroxycodone, 5.0 (8.5% and 4.6%). (C) 2004 Elsevier B.V. All rights reserved. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Palavras-Chave | #Biochemical Research Methods #Chemistry, Analytical #Morphine #Morphine-3-glucuronide #Oxycodone #Noroxycodone #High Performance Liquid Chromatography #Electrospray Ionization #Tandem Mass Spectrometry #Solid-phase Extraction #Controlled-release Oxycodone #Human Plasma #Electrochemical Detection #Cerebrospinal-fluid #Cancer Pain #Morphine-6-glucuronide #Metabolites #Pharmacokinetics #Glucuronides #C1 #320501 Pharmaceutical Sciences and Pharmacy #730104 Nervous system and disorders |
Tipo |
Journal Article |