Copper sensitivity of cueO mutants of Escherichia coli K-12 and the biochemical suppression of this phenotype


Autoria(s): Tree, J. J.; Kidd, S. P.; Jennings, M. P.; McEwan, A. G.
Data(s)

01/01/2005

Resumo

The cueO gene of Escherichia coli encodes a multi-copper oxidase, which contributes to copper tolerance in this bacterium. It was observed that a cueO mutant was highly sensitive to killing by copper ions when cells were grown on defined minimal media. Copper sensitivity was correlated with accumulation of copper in the mutant strain. Growth of the cueO mutant in the presence of copper could be restored by addition of divalent zinc and manganese ions or ferrous iron but not by other first row transition metal ions or magnesium ions. Copper toxicity towards a cueO mutant Could also be suppressed by addition of the superoxide quencher 1,2-dihydroxybenzene-3,5-disulfonic acid (tiron), suggesting that a primary cause of copper toxicity is the copper-catalyzed production of superoxide anions in the cytoplasm. (C) 2005 Elsevier Inc. All rights reserved.

Identificador

http://espace.library.uq.edu.au/view/UQ:76281

Idioma(s)

eng

Publicador

Academic Press Inc Elsevier Science

Palavras-Chave #Copper Toxicity #Escherichia Coli #Multi-copper Oxidase #Biochemistry & Molecular Biology #Biophysics #Iron Transport-system #Multicopper Oxidase #Saccharomyces-cerevisiae #Molecular-mechanisms #Induced Damage #Oxidation #Siderophores #Superoxide #Expression #Tolerance #C1 #730101 Infectious diseases #270306 Microbial Genetics
Tipo

Journal Article