Metal Clips Induce Folding of a Short Unstructured Peptide into an a-Helix via Turn Conformations in Water. Kinetic versus Thermodynamic Products
Contribuinte(s) |
Peter Stang |
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Data(s) |
01/01/2004
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Resumo |
Short peptides corresponding to two to four a-helical turns of proteins are not thermodynamically stable helices in water. Unstructured octapeptide Ac-His1*-Ala2-Ala3-His4*-His5*-Glu6-Leu7-His8*-NH2 (1) reacts with two [Pd ((NH2)-N-15(CH2)(2) (NH2)-N-15)(NO3)(2)] in water to form a kinetically stable intermediate, [{Pden}(2)-{(1,4)(5,8)-peptide}](2), in which two 19-membered metallocyclic rings stabilize two peptide turns. Slow subsequent folding to a thermodynamically more stable two-turn a-helix drives the equilibrium to [{Pden}(2)-{(1,5)(4,8)-peptide}] (3), featuring two 22-membered rings. This transformation from unstructured peptide via turns to an a-helix suggests that metal clips might be useful probes for investigating peptide folding. |
Identificador | |
Idioma(s) |
eng |
Publicador |
American Chemical Society |
Palavras-Chave | #Chemistry, Multidisciplinary #Nuclear-magnetic-resonance #Protein Secondary Structure #Ion Enhanced Helicity #Amino-acids #Coupling-constants #Copper(ii) Complexes #Stability-constants #Circular-dichroism #Crystal-structure #Nmr-spectroscopy #C1 #250301 Organic Chemical Synthesis #780103 Chemical sciences |
Tipo |
Journal Article |