Novel roles for the AIDA adhesion from diarrheagenic Escherichia coli: Cell aggregation and biofilm formation


Autoria(s): Sherlock, Orla; Schembri, Mark A.; Reisner, Andreas; Klemm, Per
Data(s)

01/12/2004

Resumo

Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43) -expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers.

Identificador

http://espace.library.uq.edu.au/view/UQ:72339/UQ72339_OA.pdf

http://espace.library.uq.edu.au/view/UQ:72339

Idioma(s)

eng

Publicador

American Society for Microbiology

Palavras-Chave #Diffuse Adherence #Antigen 43 #Autotransporter Proteins #Bordetella-pertussis #Strain-2787 O126-h27 #Bacterial Adhesins #Colony Morphology #Type-1 Fimbriae #Hep-2 Cells #Virulence
Tipo

Journal Article