Microwave-Assisted Extraction of Phenolic Compounds from Almond Skin Byproducts (Prunus amygdalus): A Multivariate Analysis Approach


Autoria(s): Valdés García, Arantzazu; Vidal, Lorena; Beltrán Sanahuja, Ana; Canals Hernández, Antonio; Garrigós Selva, María del Carmen
Contribuinte(s)

Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología

Análisis de Polímeros y Nanomateriales

Espectroscopía Atómica-Masas y Química Analítica en Condiciones Extremas

Data(s)

15/06/2015

15/06/2015

25/05/2015

Resumo

A microwave-assisted extraction (MAE) procedure to isolate phenolic compounds from almond skin byproducts was optimized. A three-level, three-factor Box–Behnken design was used to evaluate the effect of almond skin weight, microwave power, and irradiation time on total phenolic content (TPC) and antioxidant activity (DPPH). Almond skin weight was the most important parameter in the studied responses. The best extraction was achieved using 4 g, 60 s, 100 W, and 60 mL of 70% (v/v) ethanol. TPC, antioxidant activity (DPPH, FRAP), and chemical composition (HPLC-DAD-ESI-MS/MS) were determined by using the optimized method from seven different almond cultivars. Successful discrimination was obtained for all cultivars by using multivariate linear discriminant analysis (LDA), suggesting the influence of cultivar type on polyphenol content and antioxidant activity. The results show the potential of almond skin as a natural source of phenolics and the effectiveness of MAE for the reutilization of these byproducts.

We acknowledge the Spanish Ministry of Economy and Competitiveness for financial support (MAT-2011-28468-C02-01). A.V. thanks Conselleria de Educación (Spain) for ACIF/2010/172 Predoctoral Research Training Grant.

Identificador

Journal of Agricultural and Food Chemistry. 2015, 63(22): 5395-5402. doi:10.1021/acs.jafc.5b01011

0021-8561 (Print)

1520-5118 (Online)

http://hdl.handle.net/10045/47518

10.1021/acs.jafc.5b01011

Idioma(s)

eng

Publicador

American Chemical Society

Relação

http://dx.doi.org/10.1021/acs.jafc.5b01011

Direitos

© 2015 American Chemical Society

info:eu-repo/semantics/openAccess

Palavras-Chave #Microwave-assisted extraction #Phenolic compounds #Almond skin #HPLC-DAD-ESI-MS/MS #Antioxidant activity #Linear discriminant analysis #Química Analítica #Nutrición y Bromatología
Tipo

info:eu-repo/semantics/article