Assembly of the neutrophil respiratory burst oxidase: A direct interaction between p67PHOX and cytochrome b558
Data(s) |
13/03/2001
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Resumo |
Activation of the phagocyte NADPH oxidase complex requires the assembly of the cytosolic factors p47PHOX, p67PHOX, p40PHOX, and Rac1 or Rac2, with the membrane-bound cytochrome b558. Whereas the interaction of p47PHOX with cytochrome b558 is well established, an interaction between p67PHOX and cytochrome b558 has never been investigated. We report here a direct interaction between p67PHOX and cytochrome b558. First, labeled p67PHOX recognizes a 91-kDa band in specific granules from a normal patient but not from a cytochrome b558-deficient patient. Second, p67PHOX binds to cytochrome b558 that has been bound to nitrocellulose. Third, GTP-p67PHOX bound to glutathione agarose is able to pull down cytochrome b558. Rac1-GTP or Rac1-GDP increased the binding of p67PHOX to cytochrome b558, suggesting that at least one of the oxidase-related functions of Rac1 is to promote the interaction between p67PHOX and cytochrome b558. |
Identificador |
/pmc/articles/PMC30596/ /pubmed/11248021 |
Idioma(s) |
en |
Publicador |
The National Academy of Sciences |
Direitos |
Copyright © 2001, The National Academy of Sciences |
Palavras-Chave | #Biological Sciences |
Tipo |
Text |