A Toolbox for Quantitative Gene Expression in Varroa destructor : RNA Degradation in Field Samples and Systematic Analysis of Reference Gene Stability
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17/05/2016
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16/05/2016
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| Resumo |
Funding: This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 613960 (SMARTBEES) (http://www.smartbees-fp7.eu/) and Veterinary Medicines Directorate, Department for Environment Food & Rural Affairs (Project # VM0517) (https://www.gov.uk/government/organisations/veterinary-medicines-directorate). CHM was supported by a Biosciences Knowledge Transfer Network Biotechnology and Biological Sciences Research Council (KTN-BBSRC CASE) Studentship (BB/L502467/1) (http://www.bbsrc.ac.uk/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments We gratefully acknowledge Mr Sebastian Bacz’s expert help and advice with beekeeping. Peer reviewed Publisher PDF |
| Identificador |
Campbell , E M , McIntosh , C H & Bowman , A S 2016 , ' A Toolbox for Quantitative Gene Expression in Varroa destructor : RNA Degradation in Field Samples and Systematic Analysis of Reference Gene Stability ' PLoS ONE , vol 11 , no. 5 , 0155640 . , 10.1371/journal.pone.0155640 1932-6203 PURE: 66511979 PURE UUID: e033daee-b251-4ad5-8168-2b48d2a13a76 |
| Idioma(s) |
eng |
| Relação |
PLoS ONE |
| Direitos |
Copyright: © 2016 Campbell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ |
| Palavras-Chave | #varroa destructor #apis mellifera #honey bee #honey bee mite #qPCR #reference gene #normalizing gene #MIQE #RNA quality #RNA integrity #sample collection #QH301 Biology #Biotechnology and Biological Sciences Research Council (BBSRC) #BB/L502467/1 #QH301 |
| Tipo |
Journal article |
| Contribuinte(s) |
University of Aberdeen, School of Biological Sciences, Biological Sciences University of Aberdeen, MASTS (Marine Alliance for Science and Technology for Scotland) |
