Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy


Autoria(s): Luengo Oroz, Miguel Ángel; Rubio Guivernau, José Luis; Faure, Emmanuel; Savy, Thierry; Duloquin, Louise; Olivier, Nicolas; Pastor Escuredo, David; Ledesma Carbayo, María Jesús; Debarre, Delphine; Bourgine, Paul; Beaurepaire, Emmanuel; Peyrieras, Nadine; Santos Lleo, Andres de
Data(s)

01/04/2012

Resumo

Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.

Formato

application/pdf

Identificador

http://oa.upm.es/15972/

Idioma(s)

eng

Publicador

E.T.S.I. Telecomunicación (UPM)

Relação

http://oa.upm.es/15972/1/INVE_MEM_2012_131722.pdf

http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6093965

info:eu-repo/semantics/altIdentifier/doi/10.1109/TIP.2011.2177911

Direitos

http://creativecommons.org/licenses/by-nc-nd/3.0/es/

info:eu-repo/semantics/openAccess

Fonte

IEEE Transactions on Image Processing, ISSN 1057-7149, 2012-04, Vol. 21, No. 4

Palavras-Chave #Telecomunicaciones #Biología
Tipo

info:eu-repo/semantics/article

Artículo

PeerReviewed