Oligotrophic bacteria from the Antarctic Ocean
Cobertura |
MEDIAN LATITUDE: -66.459184 * MEDIAN LONGITUDE: 27.889713 * SOUTH-BOUND LATITUDE: -67.735000 * WEST-BOUND LONGITUDE: 8.890000 * NORTH-BOUND LATITUDE: -64.956700 * EAST-BOUND LONGITUDE: 37.460000 * DATE/TIME START: 1990-03-24T18:30:00 * DATE/TIME END: 1990-04-15T18:55:00 |
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Data(s) |
10/09/1999
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Resumo |
From enrichment cultures in dialysis chambers held in natural seawater tanks, 104 strains were isolated and kept in culture. All strains proved to be Gram-negative and psychrotrophic, having optimum growth temperatures of between 20 and 24 °C. Maximal growth temperatures were 30 to 37 °C, or even higher. With 55 isolates, substrate utilizations in Biolog MicroPlates were determined, and the obtained metabolic fingerprints used for clustering. Five groups could be distinguished at the 80% similarity level. Fifteen strains belonged to cluster 1, seven strains to cluster 2, and each of the clusters 3 and 4 contained nine strains. Cluster 5 can be divided into subcluster 5a and 5b, with 6 strains showing a few substrates metabolized, and 9 strains without any reactions, or weak reactions for one or two substrates, respectively. Each cluster could be characterized by specific metabolic fingerprints. Strains from cluster 1 metabolized N-acetyl-D-glucosamine, alpha-hydroxybutyric acid and gamma-hydroxybutyric acid, strains from cluster 2 citric acid, formic acid, thymidine and putrescine, strains from cluster 3 glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-threonine and inosine, whereas strains from cluster 4 metabolized alpha-cyclodextrin and N-acetyl-D-galactosamine, typically. Methylamine was not utilized by the isolates, but strains from cluster 1, 2 and 3 could grow on basal seawater agar. Morphological characteristics and photomicrographs of the oligotrophic strains are presented. Due to their typical morphologies and ampicillin resistence, the nine strains from cluster 3 can be regarded as new species of the genus Planctomyces. These bacteria have not been cultivated before. |
Formato |
application/zip, 2 datasets |
Identificador |
https://doi.pangaea.de/10.1594/PANGAEA.745115 doi:10.1594/PANGAEA.745115 |
Idioma(s) |
en |
Publicador |
PANGAEA |
Relação |
Tan, Tjhing Lok; Rüger, Hans-Jürgen (1999): Enrichment, isolation, and Biolog metabolic fingerprints of oligotrophic bacteria from the Antarctic Ocean. Advances in Limnology, 54, 255-272 |
Direitos |
CC-BY: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted |
Palavras-Chave | #-; ANT-VIII/6; Cluster analysis; Cosmonaut Sea; DEPTH, water; Depth water; Event; Indian Ocean; Label; Lazarev Sea; Metabolic fingerprints of oligotrophic bacteria from cluster 1; Metabolic fingerprints of oligotrophic bacteria from cluster 2; Metabolic fingerprints of oligotrophic bacteria from cluster 3; Metabolic fingerprints of oligotrophic bacteria from cluster 4; Polarstern; PS16; PS16/507; PS16/509; PS16/518; PS16/526; PS16/530; PS16/534; PS16/535; PS16/547; PS16/549; PS16/554; PS16/557; Riiser-Larsen Sea; Sample code/label; South Atlantic Ocean; Strain number; Substrate; Substrate type; Water sample; WS |
Tipo |
Dataset |