Respiration rates of tropical Atlantic copepods from the Cape Verde Islands

Copepods were sampled at two sampling sites off the island of São Vicente, Cape Verde Archipelago, in spring (March/April) and early summer (May/June) of 2010. The two sampling sites were located in Mindelo Bay (16.90N, 25.01W; bottom depth 22 m) and around 8 km off the town of São Pedro (16.77N, 25.12W; bottom depth 800 m). Samples were collected on board the local fishing vessel 'Sinagoga' using a WP-2 net (Hydrobios, 0.26 m**2 mouth opening, 200 µm mesh size). The net was either applied as a driftnet, drifting for 10 min in 22 to 0 m depth below the surface, or it was towed vertically with a towing speed of 0.5 m/s**1. For stratified sampling, the net was deployed in repetitive hauls from 560 to 210 m, from 210 to 80 m, and from 80 to 0 m in March/April and from 600 to 300 m, 300 to 100 m, and 100 to 0 m in May/June. Additional depth-integrated hauls were conducted from 600-0 m or 500-0 m during both field campaigns. Respiration rates of epi- and mesopelagic calanoid copepods were measured in the land-based laboratory at the Instituto Nacional de Desenvolvimento das Pescas (INDP) in Mindelo. Oxygen consumption was measured non-invasively by optode respirometry at three different ambient temperatures (13, 18, and 23°C) with a 10-channel oxygen respirometer (Oxy-10 Mini, PreSens Precision Sensing GmbH, Regensburg, Germany). All experiments were run in darkness in temperature-controlled incubators (LMS Cooled Incubator Series 1A, Model 280) equipped with water baths to ensure constant temperatures throughout the experiments, tolerating a variation of ±1°C.